Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0605(Antihypertensive peptide)

DFBP ID	DFBPANHY0605
Peptide sequence	IWHHT
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ile-Trp-His-His-Thr

Single-letter amino acid

IWHHT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	692.78 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.7000^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Dried bonito
Precursor protein	Dried bonito hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2751 ---- Plant proteins ---- Actin-7 Source.2: DFBPPR2763 ---- Plant proteins ---- Actin-1 Source.3: DFBPPR2989 ---- Plant proteins ---- Actin-2 Source.4: DFBPPR3087 ---- Plant proteins ---- Actin-3 Source.5: DFBPPR3693 ---- Plant proteins ---- Probable potassium transporter 9 Source.6: DFBPPR5185 ---- Plant proteins ---- Actin-1 Source.7: DFBPPR5283 ---- Plant proteins ---- Actin-3 Source.8: DFBPPR6550 ---- Plant proteins ---- Actin-3 Source.9: DFBPPR6551 ---- Plant proteins ---- Actin-2 Source.10: DFBPPR6552 ---- Plant proteins ---- Actin-1 Source.11: DFBPPR16188 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.12: DFBPPR17154 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.13: DFBPPR17155 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.14: DFBPPR17514 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.15: DFBPPR17747 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.16: DFBPPR18053 ---- Animal proteins ---- Amiloride-sensitive sodium channel subunit beta Source.17: DFBPPR8884 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.18: DFBPPR10103 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.19: DFBPPR10319 ---- Animal proteins ---- Actin, alpha cardiac muscle 1 Source.20: DFBPPR10338 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.21: DFBPPR10777 ---- Animal proteins ---- Actin, cytoplasmic type 5 Source.22: DFBPPR10810 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.23: DFBPPR12268 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.24: DFBPPR12700 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.25: DFBPPR12701 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.26: DFBPPR13231 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.27: DFBPPR13621 ---- Animal proteins ---- Amiloride-sensitive sodium channel subunit beta Source.28: DFBPPR13769 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.29: DFBPPR13996 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.30: DFBPPR14021 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.31: DFBPPR14143 ---- Marine protein ---- Actin, cytoplasmic 1 Source.32: DFBPPR14935 ---- Microorganism protein ---- Actin Source.33: DFBPPR7844 ---- Plant protein ---- Actin-1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

Table 1. Inhibitory activities for antihypertensive activities of peptides following intravenous administration in SHR^[1]
Peptide	Dose (mg/kg)	Antihypertensive activity (△mmHg, i.v.*)	Time
IWHHT	10	-60	6 h
	60	-26
* Decrease of systolic blood pressure after intravenous administration

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Bonito protein was hydrolyzed with Thermolysin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0012
[D2]	DFBPMUFU0004

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Iroyukifujita, H., Eiichiyokoyama, K., Yoshikawa, M. Classification and Antihypertensive Activity of Angiotensin I-Converting Enzyme Inhibitory Peptides Derived from Food Proteins. Journal of Food Science. 2000, 65, 564-9. DOI: 10.1111/j.1365-2621.2000.tb16049.x
Other literature(s)	[1] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361.
PubDate	2000