Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0657(Antihypertensive peptide)

DFBP ID	DFBPANHY0657
Peptide sequence	GVGY
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Val-Gly-Tyr

Single-letter amino acid

GVGY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	394.43 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.5250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Silkworm fibroin
Precursor protein	Silkworm fibroin hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0812 ---- Plant proteins ---- ATP-dependent DNA helicase MER3 homolog Source.2: DFBPPR0897 ---- Plant proteins ---- Nuclear transcription factor Y subunit B-11 Source.3: DFBPPR1270 ---- Plant proteins ---- Brassinosteroid LRR receptor kinase BRL1 Source.4: DFBPPR1843 ---- Plant proteins ---- Laccase-13 Source.5: DFBPPR1848 ---- Plant proteins ---- Chitinase 7 Source.6: DFBPPR1882 ---- Plant proteins ---- Laccase-12 Source.7: DFBPPR1888 ---- Plant proteins ---- Cytokinin dehydrogenase 11 Source.8: DFBPPR2017 ---- Plant proteins ---- Asparagine synthetase [glutamine-hydrolyzing] 1 Source.9: DFBPPR2062 ---- Plant proteins ---- Asparagine synthetase [glutamine-hydrolyzing] 2 Source.10: DFBPPR2077 ---- Plant proteins ---- Probable cinnamyl alcohol dehydrogenase 8C Source.11: DFBPPR2204 ---- Plant proteins ---- Probable glucomannan 4-beta-mannosyltransferase 9 Source.12: DFBPPR2211 ---- Plant proteins ---- Probable mixed-linked glucan synthase 3 Source.13: DFBPPR2271 ---- Plant proteins ---- Probable serine/threonine-protein kinase WNK3 Source.14: DFBPPR2594 ---- Plant proteins ---- Protein HAPLESS 2-A Source.15: DFBPPR2596 ---- Plant proteins ---- Probable cinnamyl alcohol dehydrogenase 9 Source.16: DFBPPR2771 ---- Plant proteins ---- Auxin response factor 9 Source.17: DFBPPR2792 ---- Plant proteins ---- Probable cinnamyl alcohol dehydrogenase 8A Source.18: DFBPPR2814 ---- Plant proteins ---- Probable cinnamyl alcohol dehydrogenase 8D Source.19: DFBPPR2909 ---- Plant proteins ---- Probable isoprenylcysteine alpha-carbonyl methylesterase ICME Source.20: DFBPPR2968 ---- Plant proteins ---- Probable aquaporin PIP2-7 Source.21: DFBPPR3151 ---- Plant proteins ---- Protein HAPLESS 2-B Source.22: DFBPPR3349 ---- Plant proteins ---- Outer envelope protein 80, chloroplastic Source.23: DFBPPR3693 ---- Plant proteins ---- Probable potassium transporter 9 Source.24: DFBPPR3842 ---- Plant proteins ---- Probable protein phosphatase 2C 39 Source.25: DFBPPR4187 ---- Plant proteins ---- Barley B recombinant-like protein C Source.26: DFBPPR4751 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 30 Source.27: DFBPPR5556 ---- Plant proteins ---- Thiamine thiazole synthase 2, chloroplastic Source.28: DFBPPR5685 ---- Plant proteins ---- Protein ABERRANT POLLEN TRANSMISSION 1 Source.29: DFBPPR5703 ---- Plant proteins ---- Asparagine synthetase [glutamine-hydrolyzing] Source.30: DFBPPR6245 ---- Plant proteins ---- Glycine dehydrogenase (decarboxylating), mitochondrial Source.31: DFBPPR6336 ---- Plant proteins ---- Asparagine synthetase, root [glutamine-hydrolyzing] Source.32: DFBPPR6338 ---- Plant proteins ---- Asparagine synthetase, nodule [glutamine-hydrolyzing] Source.33: DFBPPR6647 ---- Plant proteins ---- 2-carboxy-D-arabinitol-1-phosphatase Source.34: DFBPPR7084 ---- Plant proteins ---- 26 kDa endochitinase 2 Source.35: DFBPPR7121 ---- Plant proteins ---- 26 kDa endochitinase 1 Source.36: DFBPPR7162 ---- Plant proteins ---- Serine carboxypeptidase II-3 Source.37: DFBPPR7166 ---- Plant proteins ---- Serine carboxypeptidase II-2 Source.38: DFBPPR7405 ---- Plant proteins ---- Sinapine esterase Source.39: DFBPPR8449 ---- Plant proteins ---- Basic endochitinase C Source.40: DFBPPR16986 ---- Animal proteins ---- Aspartyl/asparaginyl beta-hydroxylase Source.41: DFBPPR17038 ---- Animal proteins ---- NAD(P) transhydrogenase, mitochondrial Source.42: DFBPPR17350 ---- Animal proteins ---- DNA mismatch repair protein Msh2 Source.43: DFBPPR17456 ---- Animal proteins ---- Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Source.44: DFBPPR17585 ---- Animal proteins ---- Calcium-transporting ATPase type 2C member 1 Source.45: DFBPPR17827 ---- Animal proteins ---- Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial Source.46: DFBPPR17924 ---- Animal proteins ---- Sodium-dependent dopamine transporter Source.47: DFBPPR18209 ---- Animal proteins ---- Sodium-dependent noradrenaline transporter Source.48: DFBPPR18340 ---- Animal proteins ---- Voltage-dependent anion-selective channel protein 2 Source.49: DFBPPR18620 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.50: DFBPPR18767 ---- Animal proteins ---- Toll-interacting protein Source.51: DFBPPR19835 ---- Animal proteins ---- GMP reductase 2 Source.52: DFBPPR20029 ---- Animal proteins ---- Sodium- and chloride-dependent glycine transporter 1 Source.53: DFBPPR20333 ---- Animal proteins ---- RNA-binding protein 14 Source.54: DFBPPR20658 ---- Animal proteins ---- G-protein coupled receptor family C group 5 member C Source.55: DFBPPR21609 ---- Animal proteins ---- Protein PHTF1 Source.56: DFBPPR9329 ---- Animal proteins ---- Voltage-dependent anion-selective channel protein 2 Source.57: DFBPPR9580 ---- Animal proteins ---- Mediator of DNA damage checkpoint protein 1 Source.58: DFBPPR10026 ---- Animal proteins ---- Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Source.59: DFBPPR11153 ---- Animal proteins ---- Toll-interacting protein Source.60: DFBPPR11967 ---- Animal proteins ---- Monocarboxylate transporter 4 Source.61: DFBPPR12221 ---- Animal proteins ---- Coiled-coil domain-containing protein 174 Source.62: DFBPPR12693 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.63: DFBPPR13065 ---- Animal proteins ---- Tartrate-resistant acid phosphatase type 5 Source.64: DFBPPR13617 ---- Animal proteins ---- NAD(P) transhydrogenase, mitochondrial Source.65: DFBPPR13914 ---- Animal proteins ---- Homeobox protein Hox-C6 Source.66: DFBPPR14173 ---- Marine protein ---- Homeobox protein Hox-A5 Source.67: DFBPPR14177 ---- Marine protein ---- Toll-interacting protein Source.68: DFBPPR14434 ---- Marine protein ---- 50S ribosomal protein L6, chloroplastic Source.69: DFBPPR14668 ---- Marine protein ---- Toll-interacting protein A Source.70: DFBPPR14671 ---- Marine protein ---- Toll-interacting protein B Source.71: DFBPPR14902 ---- Microorganism protein ---- Lysophospholipase Source.72: DFBPPR15113 ---- Microorganism protein ---- NADH-cytochrome b5 reductase 1 Source.73: DFBPPR8035 ---- Plant protein ---- Endochitinase Source.74: DFBPPR8058 ---- Plant protein ---- Endochitinase CH5B Source.75: DFBPPR8121 ---- Plant protein ---- Glycine-rich cell wall structural protein 1.8
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

Table 1 The antihypertensive activity of synthetic peptide.
Peptide	In vitro experiments	Dose (mg/kg)	SBP decrease (mm Hg)
GVGY	SHR	44	-33.0

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Fibroin, which was hydrolyzed with alcalase after partial hydrolysis with hot aqueous 40% CaCl₂, released two major active peptides showing ACE-inhibitory activity.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	No measured
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Igarashi K, Yoshioka K, Mizutani K, Miyakoshi M, Murakami T, Akizawa T. Blood pressure-depressing activity of a peptide derived from silkworm fibroin in spontaneously hypertensive rats. Biosci Biotechnol Biochem. 2006 Feb;70(2):517-20. PMID: 16495671 DOI: 10.1271/bbb.70.517
Other literature(s)	[1] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361.
PubDate	2006