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List of peptide properties
DFBP ID - DFBPANHY0692(Antihypertensive peptide)
DFBP ID DFBPANHY0692
Peptide sequence MRW
Type Native peptide
Peptide/Function name Antihypertensive peptide, Rubimetide
Function-activity relationship
Main bioactivity Antihypertensive activity
Otheir bioactivity ACE-inhibitory activity [D1], Anxiolytic-like activity [D2], Multifunctional activity [D3]
Calculated physicochemical properties
Three-letter amino acid Met-Arg-Trp
Single-letter amino acid MRW
Peptide length 3
Peptide mass
Experimental mass Theoretical mass
N.D 491.61 Da c
Net charge 0.00 c
Isoelectric point (pI) 11.04 c
IC50 N.D
pIC50 N.D
GRAVY -1.1667 c
Hydrophilic residue ratio 66.67% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Plant
Organism/Source Spinach Rubisco
Precursor protein Spinach ribulose bisphosphate carboxylase/oxygenase (Rubisco)
Residue position N.D
Precursor protein(s) search
Source.1: DFBPPR0049 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.2: DFBPPR0923 ---- Plant proteins ---- Lysine-specific demethylase JMJ703
Source.3: DFBPPR0974 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.4: DFBPPR1008 ---- Plant proteins ---- Cellulose synthase A catalytic subunit 9 [UDP-forming]
Source.5: DFBPPR1277 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 8 [UDP-forming]
Source.6: DFBPPR1343 ---- Plant proteins ---- Transcription factor TB1
Source.7: DFBPPR1452 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 2 [UDP-forming]
Source.8: DFBPPR1455 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 5 [UDP-forming]
Source.9: DFBPPR1461 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 3 [UDP-forming]
Source.10: DFBPPR1462 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 6 [UDP-forming]
Source.11: DFBPPR1486 ---- Plant proteins ---- Probable transcription factor RL9
Source.12: DFBPPR1695 ---- Plant proteins ---- Putative cellulose synthase A catalytic subunit 11 [UDP-forming]
Source.13: DFBPPR1788 ---- Plant proteins ---- Beta-galactosidase 6
Source.14: DFBPPR1792 ---- Plant proteins ---- Probable 3-ketoacyl-CoA synthase 20
Source.15: DFBPPR1871 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 17
Source.16: DFBPPR1875 ---- Plant proteins ---- Protein PHOSPHATE STARVATION RESPONSE 2
Source.17: DFBPPR1955 ---- Plant proteins ---- Endoribonuclease Dicer homolog 3b
Source.18: DFBPPR2071 ---- Plant proteins ---- Auxin response factor 11
Source.19: DFBPPR2211 ---- Plant proteins ---- Probable mixed-linked glucan synthase 3
Source.20: DFBPPR2340 ---- Plant proteins ---- Protein NODULATION SIGNALING PATHWAY 1
Source.21: DFBPPR2422 ---- Plant proteins ---- 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic
Source.22: DFBPPR2435 ---- Plant proteins ---- 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic
Source.23: DFBPPR2646 ---- Plant proteins ---- CBL-interacting protein kinase 25
Source.24: DFBPPR2702 ---- Plant proteins ---- Beta-glucosidase 27
Source.25: DFBPPR2863 ---- Plant proteins ---- Serine carboxypeptidase-like
Source.26: DFBPPR2972 ---- Plant proteins ---- Probable potassium transporter 14
Source.27: DFBPPR3248 ---- Plant proteins ---- Endoglucanase 16
Source.28: DFBPPR3502 ---- Plant proteins ---- Protein PHOSPHATE STARVATION RESPONSE 1
Source.29: DFBPPR3528 ---- Plant proteins ---- Zinc transporter 2
Source.30: DFBPPR3609 ---- Plant proteins ---- Thioredoxin-like 1-1, chloroplastic
Source.31: DFBPPR4097 ---- Plant proteins ---- Trafficking protein particle complex II-specific subunit 130 homolog
Source.32: DFBPPR4249 ---- Plant proteins ---- 5'-adenylylsulfate reductase-like 2
Source.33: DFBPPR4326 ---- Plant proteins ---- Protein BIG GRAIN 1-like
Source.34: DFBPPR4580 ---- Plant proteins ---- Ricin B-like lectin R40G3
Source.35: DFBPPR4988 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.36: DFBPPR5427 ---- Plant proteins ---- Transcription factor TEOSINTE BRANCHED 1
Source.37: DFBPPR5537 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.38: DFBPPR5825 ---- Plant proteins ---- UDP-glycosyltransferase 708A6
Source.39: DFBPPR6233 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.40: DFBPPR6627 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.41: DFBPPR7037 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.42: DFBPPR7728 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.43: DFBPPR8374 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.44: DFBPPR8427 ---- Plant proteins ---- Ribulose bisphosphate carboxylase large chain
Source.45: DFBPPR16254 ---- Animal proteins ---- Transferrin receptor protein 1
Source.46: DFBPPR16255 ---- Animal proteins ---- Frizzled-6
Source.47: DFBPPR16504 ---- Animal proteins ---- Gap junction gamma-1 protein
Source.48: DFBPPR16963 ---- Animal proteins ---- Lysosomal alpha-glucosidase
Source.49: DFBPPR17542 ---- Animal proteins ---- Acetylcholine receptor subunit beta
Source.50: DFBPPR18317 ---- Animal proteins ---- G-protein coupled receptor 37-like 1
Source.51: DFBPPR18383 ---- Animal proteins ---- Transcription factor E3
Source.52: DFBPPR18704 ---- Animal proteins ---- Phosphatidylinositol-3-phosphatase SAC1
Source.53: DFBPPR19428 ---- Animal proteins ---- CAAX prenyl protease 2
Source.54: DFBPPR19787 ---- Animal proteins ---- 60S ribosomal protein L11
Source.55: DFBPPR20037 ---- Animal proteins ---- Mitochondrial-processing peptidase subunit beta
Source.56: DFBPPR20269 ---- Animal proteins ---- Ras-related and estrogen-regulated growth inhibitor
Source.57: DFBPPR20365 ---- Animal proteins ---- Interleukin-21
Source.58: DFBPPR20834 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 12
Source.59: DFBPPR21113 ---- Animal proteins ---- Peptidase inhibitor 16
Source.60: DFBPPR21275 ---- Animal proteins ---- Gap junction gamma-1 protein
Source.61: DFBPPR21447 ---- Animal proteins ---- Transmembrane protein 39A
Source.62: DFBPPR21606 ---- Animal proteins ---- Surfeit locus protein 6
Source.63: DFBPPR21814 ---- Animal proteins ---- Vacuolar fusion protein MON1 homolog A
Source.64: DFBPPR21865 ---- Animal proteins ---- Transcription factor EC
Source.65: DFBPPR22302 ---- Animal proteins ---- Protein angel homolog 2
Source.66: DFBPPR22385 ---- Animal proteins ---- Coiled-coil domain-containing protein 102A
Source.67: DFBPPR22525 ---- Animal proteins ---- Protein ZBED8
Source.68: DFBPPR22537 ---- Animal proteins ---- Radial spoke head 10 homolog B
Source.69: DFBPPR9093 ---- Animal proteins ---- Hemopexin
Source.70: DFBPPR9185 ---- Animal proteins ---- Epididymal secretory glutathione peroxidase
Source.71: DFBPPR9364 ---- Animal proteins ---- Gap junction gamma-1 protein
Source.72: DFBPPR9391 ---- Animal proteins ---- 60S ribosomal protein L11
Source.73: DFBPPR9608 ---- Animal proteins ---- Interleukin-21
Source.74: DFBPPR9617 ---- Animal proteins ---- Interleukin-2 receptor subunit alpha
Source.75: DFBPPR9857 ---- Animal proteins ---- Cytochrome c oxidase subunit 6C
Source.76: DFBPPR10052 ---- Animal proteins ---- Protein Wnt-11
Source.77: DFBPPR10211 ---- Animal proteins ---- Fibroblast growth factor receptor 2
Source.78: DFBPPR10335 ---- Animal proteins ---- RNA-binding protein with multiple splicing 2
Source.79: DFBPPR10390 ---- Animal proteins ---- Protein sidekick-1
Source.80: DFBPPR10393 ---- Animal proteins ---- Vitellogenin-1
Source.81: DFBPPR10411 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit
Source.82: DFBPPR10422 ---- Animal proteins ---- Collagen alpha-1(XII) chain
Source.83: DFBPPR10440 ---- Animal proteins ---- RNA N6-adenosine-methyltransferase METTL16
Source.84: DFBPPR11202 ---- Animal proteins ---- Myosin-binding protein C, fast-type
Source.85: DFBPPR11225 ---- Animal proteins ---- Ornithine decarboxylase
Source.86: DFBPPR11277 ---- Animal proteins ---- Abasic site processing protein HMCES
Source.87: DFBPPR11511 ---- Animal proteins ---- E3 ubiquitin-protein ligase MIB2
Source.88: DFBPPR11526 ---- Animal proteins ---- Fibromodulin
Source.89: DFBPPR11622 ---- Animal proteins ---- Ammonium transporter Rh type C
Source.90: DFBPPR11709 ---- Animal proteins ---- Xin actin-binding repeat-containing protein 1
Source.91: DFBPPR11902 ---- Animal proteins ---- APC membrane recruitment protein 2
Source.92: DFBPPR12067 ---- Animal proteins ---- Transcription factor EC
Source.93: DFBPPR12159 ---- Animal proteins ---- Transmembrane protein 104
Source.94: DFBPPR12312 ---- Animal proteins ---- Bone morphogenetic protein 2
Source.95: DFBPPR12883 ---- Animal proteins ---- Cytochrome P450 2J1
Source.96: DFBPPR13828 ---- Animal proteins ---- Interleukin-2 receptor subunit alpha
Source.97: DFBPPR14290 ---- Marine protein ---- Ribulose bisphosphate carboxylase large chain
Source.98: DFBPPR14332 ---- Marine protein ---- Ribulose bisphosphate carboxylase large chain
Source.99: DFBPPR15022 ---- Microorganism protein ---- Pyruvate decarboxylase
Source.100: DFBPPR15600 ---- Microorganism protein ---- SVP1-like protein 2
Source.101: DFBPPR15647 ---- Microorganism protein ---- Protein IBD2
Source.102: DFBPPR0007 ---- Plant protein ---- Ribulose bisphosphate carboxylase large chain
Source.103: DFBPPR7761 ---- Plant protein ---- Ribulose bisphosphate carboxylase large chain
Source.104: DFBPPR7990 ---- Plant protein ---- Ribulose bisphosphate carboxylase large chain
Source.105: DFBPPR8038 ---- Plant protein ---- Ribulose bisphosphate carboxylase large chain
Source.106: DFBPPR8043 ---- Plant protein ---- 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic
Source.107: DFBPPR8220 ---- Plant protein ---- Ribulose bisphosphate carboxylase large chain
Link-research
Link 1: DFBPANHY0492----Spinach rubisco----Large subunit
Biological/Functional activity & target protein
Antihypertensive activity

(1) MRW reduced blood pressure after oral administration at a dose of 5 mg/kg in old SHR as well as in younger SHR. MRW exhibited vasorelaxing activity above 1 μM in isolated mesenteric artery from adult and old SHR.
(2) The hypotensive activity of MRW is mainly caused by vasorelaxation rather than by ACE-inhibition.

Specific target protein(s) N.D
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

Spinach ribulose bisphosphate carboxylase/oxygenase (Rubisco) was hydrolyzed with pepsin–pancreatin.

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: No measured
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
DFBP
[D1] DFBPACEI0046
[D2] DFBPANIP0016
[D3] DFBPMUFU0024
BIOPEP-UWM [D4] 9070
APD [D5] -
BioPepDB [D6] -
MBPDB [D7] -
Reference(s)
Primary literature Zhao H, Usui H, Ohinata K, Yoshikawa M. Met-Arg-Trp derived from Rubisco lowers blood pressure via prostaglandin D(2)-dependent vasorelaxation in spontaneously hypertensive rats. Peptides. 2008 Mar;29(3):345-9.
PMID: 18180074
Other literature(s)

[1] Yamada, Y., et al., Rapakinin, an anti-hypertensive peptide derived from rapeseed protein, dilates mesenteric artery of spontaneously hypertensive rats via the prostaglandin IP receptor followed by CCK1 receptor. Peptides, 2010. 31(5): p. 909-914.
[2] Yang Y, Marczak ED, Yokoo M, et al. Isolation and antihypertensive effect of angiotensin I-converting enzyme (ACE) inhibitory peptides from spinach Rubisco[J]. Journal of agricultural and food chemistry, 2003, 51(17):4897.

PubDate 2008
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214