Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0845(Antihypertensive peptide)

DFBP ID	DFBPANHY0845
Peptide sequence	ALAPE
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Leu-Ala-Pro-Glu

Single-letter amino acid

ALAPE

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	499.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.4600^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pearl oyster (Pinctada fucata martensii)
Precursor protein	Pearl oyster powder hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1255 ---- Plant proteins ---- Ethylene receptor 3 Source.2: DFBPPR1582 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX4 Source.3: DFBPPR5483 ---- Plant proteins ---- Caffeic acid 3-O-methyltransferase Source.4: DFBPPR18521 ---- Animal proteins ---- Prolyl 4-hydroxylase subunit alpha-3 Source.5: DFBPPR21772 ---- Animal proteins ---- RNA polymerase II-associated protein 1 Source.6: DFBPPR9252 ---- Animal proteins ---- Selenide, water dikinase 2 Source.7: DFBPPR10524 ---- Animal proteins ---- Protein disulfide-isomerase Source.8: DFBPPR12174 ---- Animal proteins ---- Protein CNPPD1 Source.9: DFBPPR14093 ---- Marine protein ---- Hepatocyte nuclear factor 1-alpha
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

The antihypertensive activity of each peptidic fraction obtained from the pearl oysters was evaluated by measuring the change in SBP at 1h, 2h, 3h, 4h, and 6h after oral administration. Systolic blood pressure did not change in the control rats during the experimental period (6 h). Captopril (10 mg/kg) lowered SBP significantly from 1 h to 4 h after administration of the drug. When the peptidic fraction from the pearl oyster was orally administered to the SHR, the fraction reduced SBP significantly by 45.9 mmHg at 3 h, and the antihypertensive effect continued for 4 h. The ACE-inhibitory peptide ALAPE was isolated from the highest active fraction, so the peptide was a potential antihypertensive peptide.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with pepsin (Porcine tomach mucosa; Wako Pure Chemicals, Osaka, Japan).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	No measured
Prediction:	ToxinPred

Additional information

The purified peptide ALAPE is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

DFBP

[D1]	DFBPACEI1180
[D2]	DFBPMUFU0237

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Suetsuna, K. Identification of antihypertensive peptides from peptic digest of the short-necked clam Tapes philippinarum and the pearl oyster Pinctada fucata martensii. Fisheries Science. 2002, 68, 233-5. DOI: 10.1046/j.1444-2906.2002.00415.x
Other literature(s)	N.D
PubDate	2002