Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0899(Antihypertensive peptide)

DFBP ID	DFBPANHY0899
Peptide sequence	LTFPG
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-Thr-Phe-Pro-Gly

Single-letter amino acid

LTFPG

Peptide length

Peptide mass

Experimental mass	Theoretical mass
534.26 Da	533.62 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.7800^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Yellow field pea (Pisum sativum L.)
Precursor protein	Provicilin
Residue position	f(171-175)
Precursor protein(s) search	Source.1: DFBPPR2126 ---- Plant proteins ---- Glutelin type-B 2 Source.2: DFBPPR2461 ---- Plant proteins ---- Glutelin type-B 1 Source.3: DFBPPR2914 ---- Plant proteins ---- Glutelin type-D 1 Source.4: DFBPPR3358 ---- Plant proteins ---- Glutelin type-B 4 Source.5: DFBPPR3666 ---- Plant proteins ---- Glutelin type-B 5 Source.6: DFBPPR6453 ---- Plant proteins ---- Convicilin Source.7: DFBPPR6499 ---- Plant proteins ---- Provicilin Source.8: DFBPPR7733 ---- Plant proteins ---- 12S seed storage globulin 2 Source.9: DFBPPR7734 ---- Plant proteins ---- 12S seed storage globulin 1 Source.10: DFBPPR16114 ---- Animal proteins ---- Collagen alpha-5(IV) chain Source.11: DFBPPR18722 ---- Animal proteins ---- Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Source.12: DFBPPR19449 ---- Animal proteins ---- SCO-spondin Source.13: DFBPPR19675 ---- Animal proteins ---- INO80 complex subunit E Source.14: DFBPPR8746 ---- Animal proteins ---- Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Source.15: DFBPPR0003 ---- Plant protein ---- Conglutin beta 2 Source.16: DFBPPR0009 ---- Plant protein ---- Conglutin beta 1 Source.17: DFBPPR7827 ---- Plant protein ---- U1 small nuclear ribonucleoprotein C-1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases	Times
SHR	30	37 mmHg	2 h
		≈ 30 mmHg	4 h
		≈ 18 mmHg	6 h
		≈ 10 mmHg	8 h
		≈ 6 mmHg	24 h

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Yellow field pea seed was hydrolyzed with Thermolysin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	No measured
Prediction:	ToxinPred

Additional information

The peptide showed 41.41 ± 0.36 % Renin (EC 3.4.23.15) inhibition (1.0 mg/mL assay concentration).

Database cross-references

DFBP

[D1]	DFBPACEI1043
[D2]	DFBPMUFU0215

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Aluko, R.E., Girgih, A.T., He, R., Malomo, S., Li, H., Offengenden, M., et al. Structural and functional characterization of yellow field pea seed (Pisum sativum L.) protein-derived antihypertensive peptides. Food Research International. 2015, 77, 10-6. DOI: 10.1016/j.foodres.2015.03.029
Other literature(s)	N.D
PubDate	2015