Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0968(Antihypertensive peptide)

DFBP ID	DFBPANHY0968
Peptide sequence	HPHPHLSF
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

His-Pro-His-Pro-His-Leu-Ser-Phe

Single-letter amino acid

HPHPHLSF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
970.5 Da	971.08 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

8.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.8750^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Ovine milk protein
Precursor protein	κ-Casein
Residue position	f(98-105)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.4: DFBPPR8492 ---- Milk proteins ---- Kappa-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

Peptide	In vitro experiments	Dose (mg/kg)	SBP decrease	DBP decrease
HPHPHLSF	SHR	10.0	-15.7 ± 2.9 mm Hg	-14.1 ± 3.1 mm Hg

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with digestive enzymes (Gastric and pancreatic enzymes pepsin, chymotrypsin and trypsin).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

The peptide was bioactive ingredient with potential benefit in the prevention and treatment of hypertension or other associated disorders.

Database cross-references

DFBP

[D1]	DFBPACEI1466
[D2]	DFBPANOX0258
[D3]	DFBPMUFU0397

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Miguel M, Gómez-Ruiz JÁ, Recio I, Aleixandre A. Changes in arterial blood pressure after single oral administration of milk-casein-derived peptides in spontaneously hypertensive rats. Mol Nutr Food Res. 2010 Oct;54(10):1422-7. PMID: 20397194 DOI: 10.1002/mnfr.200900448
Other literature(s)	[1] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361.
PubDate	2010