Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0974(Antihypertensive peptide)

DFBP ID	DFBPANHY0974
Peptide sequence	TTMPLW
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], DPP IV-inhibitory activity ^[D2], Immunomodulatory activity ^[D3], Opioid activity ^[D4], Multifunctional activity ^[D5]

Calculated physicochemical properties

Three-letter amino acid

Thr-Thr-Met-Pro-Leu-Trp

Single-letter amino acid

TTMPLW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	747.90 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.3000^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	α_s1-Casein
Residue position	f(194-199)
Precursor protein(s) search	Source.1: DFBPPR7688 ---- Milk proteins ---- Alpha-S1-casein Source.2: DFBPPR7717 ---- Milk proteins ---- Alpha-S1-casein Source.3: DFBPPR8488 ---- Milk proteins ---- Alpha-S1-casein
Link-research Inductive analysis	Link 1: DFBPANHY0140----Milk protein----α_s1-Casein

Biological/Functional activity & target protein

Antihypertensive activity

Peptide	In vitro experiments	Dose (mg/kg)	SBP decrease
TTMPLW	SHR	100.0	-13.6 mm Hg

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with trypsin

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0575
[D2]	DFBPDPIV0188
[D3]	DFBPIMMU0029
[D4]	DFBPOPIO0068
[D5]	DFBPMUFU0137

BIOPEP-UWM

[D6]

3127, 3530, 8172, 9619

APD

[D7]

BioPepDB

[D8]

MBPDB

[D9]

Reference(s)

Primary literature	Karaki H, Doi K, Sugano S, Uchiwa H, Sugai R, Murakami U, Takemoto S. Antihypertensive effect of tryptic hydrolysate of milk casein in spontaneously hypertensive rats. Comp Biochem Physiol C Comp Pharmacol Toxicol. 1990;96(2):367-71. PMID: 1980446
Other literature(s)	[1] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361.
PubDate	1990