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List of peptide properties
DFBP ID - DFBPANHY0994(Antihypertensive peptide)
DFBP ID DFBPANHY0994
Peptide sequence LDSPSEGRAPGAD
Type Native peptide
Peptide/Function name Antihypertensive peptide
Function-activity relationship
Main bioactivity Antihypertensive activity
Otheir bioactivity ACE-inhibitory activity [D1], Multifunctional activity [D2]
Calculated physicochemical properties
Three-letter amino acid Leu-Asp-Ser-Pro-Ser-Glu-Gly-Arg-Ala-Pro-Gly-Ala-Asp
Single-letter amino acid LDSPSEGRAPGAD
Peptide length 13
Peptide mass
Experimental mass Theoretical mass
1271.59 Da 1271.28 Da c
Net charge 2
Isoelectric point (pI) 3.75 c
IC50 N.D
pIC50 N.D
GRAVY -1.0154 c
Hydrophilic residue ratio 53.85% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Wine
Organism/Source Red wine (The grapes of Cabernet variety)
Precursor protein Wine lees hydrolysate
Residue position N.D
Precursor protein(s) search
No matching precursor protein found
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
Antihypertensive activity

As shown in Figure 1, peptide LDSPSEGRAPGAD exhibited potent antihypertensive activity in SBP and DBP.

AN6.png

Figure 1. Changes in spontaneously hypertensive rat's blood pressure after the intravenous administration of LDSPSEGRAPGAD; (A) SBP changes and (B) DBP changes. Different letters in the legend represent significant differences (p < 0.05). p was estimated by two-way ANOVA.

Specific target protein(s) N.D
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Non-bitter taste prediction
SMILES N.D
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

The enzymatic hydrolysis of wine lees was carried out with Flavourzyme® (enzyme/substrate ratio, 80 LAPU/g protein) for 2 h at 25 ℃, pH 4.0 and 150 rpm in a MaxQ Orbital Shaker (Thermo Fisher Scientific, Waltham, MA, USA).

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information

This peptide has a great potential as functional ingredient to manage hypertension.

Database cross-references
DFBP
[D1] DFBPACEI2250
[D2] DFBPMUFU0647
BIOPEP-UWM [D3] -
APD [D4] -
BioPepDB [D5] -
MBPDB [D6] -
Reference(s)
Primary literature Bravo FI, Mas-Capdevila A, López-Fernández-Sobrino R, Torres-Fuentes C, Mulero M, Alcaide-Hidalgo JM, Muguerza B. Identification of novel antihypertensive peptides from wine lees hydrolysate. Food Chem. 2022 Jan 1;366:130690.
PMID: 34343949
Other literature(s) N.D
PubDate 2021
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214