Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANIP0007(Anxiolytic-like peptide)

DFBP ID	DFBPANIP0007
Peptide sequence	VYLPR
Type	Native peptide
Peptide/Function name	Anxiolytic-like activity peptide, Ovolin

Function-activity relationship

Main bioactivity	Anxiolytic-like activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Tyr-Leu-Pro-Arg

Single-letter amino acid

VYLPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	646.78 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.82 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1200^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Chicken egg white protein
Precursor protein	Ovalbumin
Residue position	f(280-284)
Precursor protein(s) search	Source.1: DFBPPR4979 ---- Plant proteins ---- Seed linoleate 9S-lipoxygenase-3 Source.2: DFBPPR5005 ---- Plant proteins ---- Linoleate 9S-lipoxygenase-4 Source.3: DFBPPR6370 ---- Plant proteins ---- Seed linoleate 9S-lipoxygenase-3 Source.4: DFBPPR8437 ---- Plant proteins ---- Linoleate 9S-lipoxygenase Source.5: DFBPPR22430 ---- Animal proteins ---- UPF0561 protein C2orf68 homolog Source.6: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Source.7: DFBPPR10456 ---- Animal proteins ---- Neuroserpin Source.8: DFBPPR10950 ---- Animal proteins ---- Ovalbumin-related protein Y Source.9: DFBPPR15496 ---- Microorganism protein ---- Cell division cycle protein 123 Source.10: DFBPPR8039 ---- Plant protein ---- Linoleate 9S-lipoxygenase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anxiolytic-like activity

The anxiolytic-like activity of ovolin was inhibited by indomethacin, a cyclooxygenase (COX) inhibitor, or BWA868C, an antagonist of the DP₁ receptor for prostaglandin (PG) D₂. Ovolin-induced anxiolytic-like activity was also blocked by SCH58261 or bicuculline, antagonists of the adenosine A_2A and GABA_A receptors, respectively. Ovolin has no affinity for the DP₁, A_2A and GABA_A receptors. Taken together, ovolin may exhibit anxiolytic-like activity in a manner dependent on the PGD₂-DP₁ system coupled to the A_2A and GABA_A receptors.
VYLP did not show anxiolytic-like activity, suggesting that C-terminal Arg is necessary for the anxiolytic-like activity of ovolin. Other C-terminal truncated peptides, such as VY and VYL were also inactive. In contrast, N-terminal truncation of ovolin gradually lowered the anxiolytic-like activity. These results suggest that the C-terminal structure of ovolin seems to be more important for anxiolytic-like activity than the N-terminal.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Structure

Embedded Mol* Viewer

Docking

Embedded Mol* Viewer

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Ovalbumin from chicken egg white (Grade V, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in water (20 mg/mL), boiled for 10 min, and digested by trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Oda A, Kaneko K, Mizushige T, Lazarus M, Urade Y, Ohinata K. Characterization of ovolin, an orally active tryptic peptide released from ovalbumin with anxiolytic-like activity. J Neurochem. 2012 Jul;122(2):356-62. PMID: 22564055 DOI: 10.1111/j.1471-4159.2012.07777.x
Other literature(s)	[1] Ohinata, K., Oda, A. Novel orally active peptide Val-Tyr-Leu-Pro-Arg derived from egg white albumin with anxiolytic-like activity. Neuroscience Research. 2011, 71.
PubDate	2012