Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANIP0008(Anxiolytic-like peptide)

DFBP ID	DFBPANIP0008
Peptide sequence	YLPR
Type	Native peptide
Peptide/Function name	Anxiolytic-like activity peptide

Function-activity relationship

Main bioactivity	Anxiolytic-like activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Leu-Pro-Arg

Single-letter amino acid

YLPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	547.64 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.82 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Chicken egg white protein
Precursor protein	Ovalbumin
Residue position	f(281-284)
Precursor protein(s) search	Source.1: DFBPPR1570 ---- Plant proteins ---- MEIOTIC F-BOX protein MOF Source.2: DFBPPR1900 ---- Plant proteins ---- Fanconi-associated nuclease 1 homolog Source.3: DFBPPR2002 ---- Plant proteins ---- bZIP transcription factor 60 Source.4: DFBPPR2102 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 12 Source.5: DFBPPR2804 ---- Plant proteins ---- Protein GIGANTEA Source.6: DFBPPR2881 ---- Plant proteins ---- Probable protein NAP1 Source.7: DFBPPR3071 ---- Plant proteins ---- ABC transporter G family member 45 Source.8: DFBPPR3823 ---- Plant proteins ---- Plant intracellular Ras-group-related LRR protein 4 Source.9: DFBPPR4442 ---- Plant proteins ---- Probable sodium/metabolite cotransporter BASS5, chloroplastic Source.10: DFBPPR4853 ---- Plant proteins ---- B3 domain-containing protein LOC_Os12g40080 Source.11: DFBPPR4979 ---- Plant proteins ---- Seed linoleate 9S-lipoxygenase-3 Source.12: DFBPPR5005 ---- Plant proteins ---- Linoleate 9S-lipoxygenase-4 Source.13: DFBPPR5398 ---- Plant proteins ---- Sulfite reductase [ferredoxin], chloroplastic Source.14: DFBPPR6370 ---- Plant proteins ---- Seed linoleate 9S-lipoxygenase-3 Source.15: DFBPPR7649 ---- Milk proteins ---- Cadherin-1 Source.16: DFBPPR8426 ---- Plant proteins ---- 2S seed storage protein 1 Source.17: DFBPPR8437 ---- Plant proteins ---- Linoleate 9S-lipoxygenase Source.18: DFBPPR17474 ---- Animal proteins ---- AFG3-like protein 2 Source.19: DFBPPR18903 ---- Animal proteins ---- Glutaryl-CoA dehydrogenase, mitochondrial Source.20: DFBPPR19057 ---- Animal proteins ---- Tubulin-specific chaperone E Source.21: DFBPPR19186 ---- Animal proteins ---- Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 Source.22: DFBPPR19608 ---- Animal proteins ---- Anoctamin-4 Source.23: DFBPPR19650 ---- Animal proteins ---- Small G protein signaling modulator 3 Source.24: DFBPPR20625 ---- Animal proteins ---- DIS3-like exonuclease 1 Source.25: DFBPPR20682 ---- Animal proteins ---- 26S proteasome regulatory subunit 10B Source.26: DFBPPR20724 ---- Animal proteins ---- Exportin-2 Source.27: DFBPPR20988 ---- Animal proteins ---- Short-chain dehydrogenase/reductase family 9C member 7 Source.28: DFBPPR21050 ---- Animal proteins ---- Tudor domain-containing protein 3 Source.29: DFBPPR21446 ---- Animal proteins ---- Spermatid-specific manchette-related protein 1 Source.30: DFBPPR21492 ---- Animal proteins ---- Homeobox protein DBX1 Source.31: DFBPPR21593 ---- Animal proteins ---- Lysosomal-associated transmembrane protein 4A Source.32: DFBPPR22296 ---- Animal proteins ---- Kelch domain-containing protein 2 Source.33: DFBPPR22430 ---- Animal proteins ---- UPF0561 protein C2orf68 homolog Source.34: DFBPPR22744 ---- Animal proteins ---- Uncharacterized protein C10orf82 homolog Source.35: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.36: DFBPPR8916 ---- Animal proteins ---- Glutaryl-CoA dehydrogenase, mitochondrial Source.37: DFBPPR9256 ---- Animal proteins ---- Antibacterial protein PR-39 Source.38: DFBPPR9564 ---- Animal proteins ---- Inter-alpha-trypsin inhibitor heavy chain H2 Source.39: DFBPPR9838 ---- Animal proteins ---- Transcription factor PU.1 Source.40: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Source.41: DFBPPR10318 ---- Animal proteins ---- LIM domain kinase 1 Source.42: DFBPPR10456 ---- Animal proteins ---- Neuroserpin Source.43: DFBPPR10579 ---- Animal proteins ---- Lysine-specific demethylase 5B Source.44: DFBPPR10950 ---- Animal proteins ---- Ovalbumin-related protein Y Source.45: DFBPPR11138 ---- Animal proteins ---- Neuropeptide Y receptor type 2 Source.46: DFBPPR11252 ---- Animal proteins ---- Transcription factor RelB homolog Source.47: DFBPPR11968 ---- Animal proteins ---- AT-rich interactive domain-containing protein 5B Source.48: DFBPPR12119 ---- Animal proteins ---- Lysosomal-associated transmembrane protein 4A Source.49: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.50: DFBPPR13072 ---- Animal proteins ---- Solute carrier family 22 member 7 Source.51: DFBPPR14004 ---- Animal proteins ---- Tyrosine-protein kinase JAK1 Source.52: DFBPPR15496 ---- Microorganism protein ---- Cell division cycle protein 123 Source.53: DFBPPR15633 ---- Microorganism protein ---- Bud site selection protein 4 Source.54: DFBPPR8039 ---- Plant protein ---- Linoleate 9S-lipoxygenase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anxiolytic-like activity

YLPR did not show anxiolytic-like activity, suggesting that C-terminal Arg is necessary for the anxiolytic-like activity of ovolin. Other C-terminal truncated peptides, such as VY and VYL were also inactive. In contrast, N-terminal truncation of ovolin (YLPR, LPR, PR) gradually lowered the anxiolytic-like activity. These results suggest that the C-terminal structure of ovolin seems to be more important for anxiolytic-like activity than the N-terminal.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Structure

Embedded Mol* Viewer

Docking

Embedded Mol* Viewer

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	VYLP was synthesized by the Fmoc strategy, and purified by reverse-phase HPLC.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Oda A, Kaneko K, Mizushige T, Lazarus M, Urade Y, Ohinata K. Characterization of ovolin, an orally active tryptic peptide released from ovalbumin with anxiolytic-like activity. J Neurochem. 2012 Jul;122(2):356-62. PMID: 22564055 DOI: 10.1111/j.1471-4159.2012.07777.x
Other literature(s)	N.D
PubDate	2012