Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANIP0014(Anxiolytic-like peptide)

DFBP ID	DFBPANIP0014
Peptide sequence	SYLPPLT
Type	Native peptide
Peptide/Function name	Anxiolytic-like peptide, rALP-1(1-7)

Function-activity relationship

Main bioactivity	Anxiolytic-like activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ser-Tyr-Leu-Pro-Pro-Leu-Thr

Single-letter amino acid

SYLPPLT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	789.91 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.2286^c

Hydrophilic residue ratio

57.14%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Spinach green leaf protein
Precursor protein	Rubisco small subunit
Residue position	f(73-79)
Precursor protein(s) search	Source.1: DFBPPR1496 ---- Plant proteins ---- Ribulose bisphosphate carboxylase small chain, chloroplastic Source.2: DFBPPR2450 ---- Plant proteins ---- Ribulose bisphosphate carboxylase small chain A, chloroplastic Source.3: DFBPPR6364 ---- Plant proteins ---- Ribulose bisphosphate carboxylase small chain 3C, chloroplastic Source.4: DFBPPR6373 ---- Plant proteins ---- Ribulose bisphosphate carboxylase small chain 3A, chloroplastic Source.5: DFBPPR6382 ---- Plant proteins ---- Ribulose bisphosphate carboxylase small chain, chloroplastic Source.6: DFBPPR8238 ---- Plant protein ---- Ribulose bisphosphate carboxylase small chain, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anxiolytic-like activity	rALP-1(1-7) exhibited potent anxiolytic-like effects after oral administration. The anxiolytic-like effects of rALP-1(1-7) was blocked by a serotonin 5-HT_1A receptor antagonist. In conclusion, novel Rubisco-derived anxiolytic-like peptide rALP-1(1-7) act via independent neural pathways.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Spinach leaf protein (10 mg/ml) was digested by pepsin and pancreatin (E/S = 1/100) for 5 h at 37 ℃ under pH 7.5 for the pancreatin digest and pH 2.0 for the pepsin digest.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kimura S, Uchida T, Tokuyama Y, Hosokawa M, Nakato J, Kurabayashi A, Sato M, Suzuki H, Kanamoto R, Ohinata K. Identification of Rubisco anxiolytic-like peptides (rALPs) by comprehensive analysis of spinach green leaf protein digest. Biochem Biophys Res Commun. 2018 Nov 10;505(4):1050-1056. PMID: 30314694 DOI: 10.1016/j.bbrc.2018.09.195
Other literature(s)	N.D
PubDate	2018