Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0009(Antioxidative peptide)

DFBP ID	DFBPANOX0009
Peptide sequence	VHHANEN
Type	Native peptide
Peptide/Function name	Antioxidative peptide, P3

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-His-His-Ala-Asn-Glu-Asn

Single-letter amino acid

VHHANEN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	819.84 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5571^c

Hydrophilic residue ratio

28.57%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg
Precursor protein	Egg white albumin, Egg ovalbumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR9993 ---- Animal proteins ---- Ovalbumin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	This peptide yielded antioxidative activity against linoleic acid in an aqueous system at pH 7.0 (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolyzed with Amano S (from Bacillus subtilis)

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Tsuge, N., Eikawa, Y., Nomura, Y., Yamamoto, M., Sugisawa, K. Antioxidative Activity of Peptides Prepared by Enzymatic Hydrolysis of Egg-white Albumin. Journal of the agricultural chemical society of Japan. 1991, 65, 1635-41. DOI: 10.1271/nogeikagaku1924.65.1635
Other literature(s)	[1] Mine Y. Egg proteins and peptides in human health--chemistry, bioactivity and production.[J]. Current Pharmaceutical Design, 2007, 13(9):-.
PubDate	1991