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List of peptide properties
DFBP ID - DFBPANOX0016(Antioxidative peptide)
DFBP ID DFBPANOX0016
Peptide sequence LLPH
Type Native peptide
Peptide/Function name Antioxidative peptide
Function-activity relationship
Main bioactivity Antioxidative activity
Otheir bioactivity N.D
Calculated physicochemical properties
Three-letter amino acid Leu-Leu-Pro-His
Single-letter amino acid LLPH
Peptide length 4
Peptide mass
Experimental mass Theoretical mass
N.D 478.58 Da c
Net charge 0.00 c
Isoelectric point (pI) 7.79 c
IC50 N.D
pIC50 N.D
GRAVY 0.7000 c
Hydrophilic residue ratio 75% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Designed peptide
Organism/Source Designed peptide
Precursor protein Designed peptide based on the antioxidative peptide: LLPHH
Residue position N.D
Precursor protein(s) search
Source.1: DFBPPR0915 ---- Plant proteins ---- Kinesin-like protein KIN-4A
Source.2: DFBPPR0916 ---- Plant proteins ---- Oryzalexin D synthase
Source.3: DFBPPR1099 ---- Plant proteins ---- Ent-cassadiene C11-alpha-hydroxylase 1
Source.4: DFBPPR1212 ---- Plant proteins ---- 9-beta-pimara-7,15-diene oxidase
Source.5: DFBPPR1259 ---- Plant proteins ---- Beta-carotene isomerase D27, chloroplastic
Source.6: DFBPPR1375 ---- Plant proteins ---- Chlorophyllide a oxygenase, chloroplastic
Source.7: DFBPPR1382 ---- Plant proteins ---- Cytochrome P450 76M5
Source.8: DFBPPR1478 ---- Plant proteins ---- Linoleate 9S-lipoxygenase 2
Source.9: DFBPPR1530 ---- Plant proteins ---- Ent-kaur-16-ene synthase, chloroplastic
Source.10: DFBPPR1702 ---- Plant proteins ---- Glutelin type-A 2
Source.11: DFBPPR2035 ---- Plant proteins ---- Glutelin type-A 1
Source.12: DFBPPR2151 ---- Plant proteins ---- Glutelin type-A 3
Source.13: DFBPPR2166 ---- Plant proteins ---- Eukaryotic translation initiation factor isoform 4G-1
Source.14: DFBPPR2304 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.15: DFBPPR2482 ---- Plant proteins ---- Probable allantoinase
Source.16: DFBPPR2543 ---- Plant proteins ---- DNA topoisomerase 3-beta
Source.17: DFBPPR2770 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Source.18: DFBPPR2881 ---- Plant proteins ---- Probable protein NAP1
Source.19: DFBPPR3377 ---- Plant proteins ---- Endoglucanase 3
Source.20: DFBPPR3626 ---- Plant proteins ---- Acyl transferase 7
Source.21: DFBPPR3948 ---- Plant proteins ---- Probable anion transporter 5, chloroplastic
Source.22: DFBPPR4307 ---- Plant proteins ---- Acyl transferase 8
Source.23: DFBPPR4468 ---- Plant proteins ---- Pentatricopeptide repeat-containing protein CRP1 homolog, chloroplastic
Source.24: DFBPPR4811 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 55
Source.25: DFBPPR4975 ---- Plant proteins ---- Beta-conglycinin beta subunit 1
Source.26: DFBPPR4978 ---- Plant proteins ---- 2-hydroxyisoflavanone dehydratase
Source.27: DFBPPR4990 ---- Plant proteins ---- Beta-conglycinin alpha' subunit
Source.28: DFBPPR5025 ---- Plant proteins ---- Beta-conglycinin alpha subunit 1
Source.29: DFBPPR5035 ---- Plant proteins ---- Beta-conglycinin beta subunit 2
Source.30: DFBPPR5036 ---- Plant proteins ---- Beta-conglycinin alpha subunit 2
Source.31: DFBPPR5123 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.32: DFBPPR5128 ---- Plant proteins ---- Elongation factor Tu, chloroplastic
Source.33: DFBPPR5256 ---- Plant proteins ---- Early nodulin-70
Source.34: DFBPPR5530 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.35: DFBPPR5533 ---- Plant proteins ---- Pentatricopeptide repeat-containing protein CRP1, chloroplastic
Source.36: DFBPPR5707 ---- Plant proteins ---- indole-2-monooxygenase
Source.37: DFBPPR5708 ---- Plant proteins ---- 3-hydroxyindolin-2-one monooxygenase
Source.38: DFBPPR5709 ---- Plant proteins ---- indolin-2-one monooxygenase
Source.39: DFBPPR5768 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Source.40: DFBPPR5780 ---- Plant proteins ---- Cytochrome P450 71C3
Source.41: DFBPPR5939 ---- Plant proteins ---- 15-cis-zeta-carotene isomerase, chloroplastic
Source.42: DFBPPR6340 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.43: DFBPPR6470 ---- Plant proteins ---- Vicilin
Source.44: DFBPPR6717 ---- Plant proteins ---- Alpha-amylase/trypsin inhibitor CM3
Source.45: DFBPPR6772 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.46: DFBPPR6822 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Source.47: DFBPPR7105 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.48: DFBPPR7122 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Source.49: DFBPPR7425 ---- Plant proteins ---- Stearoyl-[acyl-carrier-protein] 9-desaturase, seed specific, chloroplastic
Source.50: DFBPPR7443 ---- Plant proteins ---- Ferritin-1, chloroplastic
Source.51: DFBPPR7621 ---- Milk proteins ---- Mucin-4
Source.52: DFBPPR8437 ---- Plant proteins ---- Linoleate 9S-lipoxygenase
Source.53: DFBPPR8454 ---- Plant proteins ---- Photosystem II CP43 reaction center protein
Source.54: DFBPPR15966 ---- Animal proteins ---- Cystic fibrosis transmembrane conductance regulator
Source.55: DFBPPR16501 ---- Animal proteins ---- F-box/LRR-repeat protein 15
Source.56: DFBPPR16530 ---- Animal proteins ---- ATP synthase subunit a
Source.57: DFBPPR16561 ---- Animal proteins ---- UDP-glucuronosyltransferase 2B31
Source.58: DFBPPR17451 ---- Animal proteins ---- Cystic fibrosis transmembrane conductance regulator
Source.59: DFBPPR17596 ---- Animal proteins ---- [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
Source.60: DFBPPR17767 ---- Animal proteins ---- Bifunctional peptidase and arginyl-hydroxylase JMJD5
Source.61: DFBPPR17880 ---- Animal proteins ---- Apolipoprotein A-IV
Source.62: DFBPPR18028 ---- Animal proteins ---- Atlastin-1
Source.63: DFBPPR18040 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase F
Source.64: DFBPPR18245 ---- Animal proteins ---- ATP synthase subunit a
Source.65: DFBPPR18260 ---- Animal proteins ---- 2-oxoglutarate dehydrogenase, mitochondrial
Source.66: DFBPPR18304 ---- Animal proteins ---- Glucose-6-phosphate isomerase
Source.67: DFBPPR18698 ---- Animal proteins ---- ATPase GET3
Source.68: DFBPPR18790 ---- Animal proteins ---- Proto-oncogene vav
Source.69: DFBPPR18802 ---- Animal proteins ---- Nuclear factor 1 B-type
Source.70: DFBPPR19257 ---- Animal proteins ---- Cytosolic iron-sulfur assembly component 3
Source.71: DFBPPR19510 ---- Animal proteins ---- Transportin-1
Source.72: DFBPPR19767 ---- Animal proteins ---- F-box/LRR-repeat protein 15
Source.73: DFBPPR20534 ---- Animal proteins ---- 60S acidic ribosomal protein P0
Source.74: DFBPPR21044 ---- Animal proteins ---- Conserved oligomeric Golgi complex subunit 7
Source.75: DFBPPR21200 ---- Animal proteins ---- RAB6A-GEF complex partner protein 2
Source.76: DFBPPR21506 ---- Animal proteins ---- Origin recognition complex subunit 3
Source.77: DFBPPR21507 ---- Animal proteins ---- Nitric oxide-associated protein 1
Source.78: DFBPPR21568 ---- Animal proteins ---- FAST kinase domain-containing protein 4
Source.79: DFBPPR21951 ---- Animal proteins ---- Protein ABHD11
Source.80: DFBPPR22094 ---- Animal proteins ---- Protein MMS22-like
Source.81: DFBPPR22152 ---- Animal proteins ---- Nucleolar protein 11
Source.82: DFBPPR22266 ---- Animal proteins ---- Vexin
Source.83: DFBPPR22389 ---- Animal proteins ---- Quinone oxidoreductase-like protein 1
Source.84: DFBPPR22432 ---- Animal proteins ---- Protein FAM124B
Source.85: DFBPPR22572 ---- Animal proteins ---- Maestro heat-like repeat-containing protein family member 1
Source.86: DFBPPR22707 ---- Animal proteins ---- Protein FAM160B1
Source.87: DFBPPR8751 ---- Animal proteins ---- Cystic fibrosis transmembrane conductance regulator
Source.88: DFBPPR8809 ---- Animal proteins ---- Lysosomal acid glucosylceramidase
Source.89: DFBPPR8813 ---- Animal proteins ---- Apolipoprotein A-IV
Source.90: DFBPPR8839 ---- Animal proteins ---- Glucose-6-phosphate isomerase
Source.91: DFBPPR9277 ---- Animal proteins ---- Nuclear factor 1 C-type
Source.92: DFBPPR9463 ---- Animal proteins ---- 60S acidic ribosomal protein P0
Source.93: DFBPPR9522 ---- Animal proteins ---- ATP synthase subunit a
Source.94: DFBPPR9527 ---- Animal proteins ---- Nuclear factor 1
Source.95: DFBPPR9727 ---- Animal proteins ---- FAST kinase domain-containing protein 4
Source.96: DFBPPR10084 ---- Animal proteins ---- Nuclear factor 1 B-type
Source.97: DFBPPR10401 ---- Animal proteins ---- Heparanase
Source.98: DFBPPR10807 ---- Animal proteins ---- Dystrophin
Source.99: DFBPPR10959 ---- Animal proteins ---- Nuclear factor 1 A-type
Source.100: DFBPPR10961 ---- Animal proteins ---- Nuclear factor 1 C-type
Source.101: DFBPPR10965 ---- Animal proteins ---- Nuclear factor 1 X-type
Source.102: DFBPPR11146 ---- Animal proteins ---- Formin
Source.103: DFBPPR11221 ---- Animal proteins ---- Zinc finger homeobox protein 4
Source.104: DFBPPR11222 ---- Animal proteins ---- FACT complex subunit SSRP1
Source.105: DFBPPR11420 ---- Animal proteins ---- 60S acidic ribosomal protein P0
Source.106: DFBPPR11551 ---- Animal proteins ---- Isoleucine--tRNA ligase, mitochondrial
Source.107: DFBPPR11746 ---- Animal proteins ---- EEF1A lysine methyltransferase 1
Source.108: DFBPPR11809 ---- Animal proteins ---- Ras-specific guanine nucleotide-releasing factor RalGPS1
Source.109: DFBPPR11984 ---- Animal proteins ---- SET and MYND domain-containing protein 4
Source.110: DFBPPR12092 ---- Animal proteins ---- Protein MMS22-like
Source.111: DFBPPR12214 ---- Animal proteins ---- Nucleolar protein 11
Source.112: DFBPPR12286 ---- Animal proteins ---- Helicase-like transcription factor
Source.113: DFBPPR12355 ---- Animal proteins ---- Glucose-6-phosphate isomerase
Source.114: DFBPPR12618 ---- Animal proteins ---- Na(+)/H(+) exchange regulatory cofactor NHE-RF3
Source.115: DFBPPR12619 ---- Animal proteins ---- Na(+)/H(+) exchange regulatory cofactor NHE-RF3
Source.116: DFBPPR12750 ---- Animal proteins ---- Anion exchange protein 2
Source.117: DFBPPR12834 ---- Animal proteins ---- B1 bradykinin receptor
Source.118: DFBPPR12905 ---- Animal proteins ---- ATP synthase subunit a
Source.119: DFBPPR12925 ---- Animal proteins ---- Methylmalonic aciduria type A homolog, mitochondrial
Source.120: DFBPPR13023 ---- Animal proteins ---- Elongator complex protein 1
Source.121: DFBPPR13341 ---- Animal proteins ---- ATP synthase subunit a
Source.122: DFBPPR13492 ---- Animal proteins ---- ATP synthase subunit a
Source.123: DFBPPR13591 ---- Animal proteins ---- Cystic fibrosis transmembrane conductance regulator
Source.124: DFBPPR13825 ---- Animal proteins ---- ATP synthase subunit a
Source.125: DFBPPR14293 ---- Marine protein ---- Photosystem II CP43 reaction center protein
Source.126: DFBPPR14333 ---- Marine protein ---- Ferredoxin-dependent glutamate synthase
Source.127: DFBPPR14912 ---- Microorganism protein ---- Heat shock factor protein
Source.128: DFBPPR14919 ---- Microorganism protein ---- Transcription elongation factor SPT4
Source.129: DFBPPR14924 ---- Microorganism protein ---- E3 ubiquitin-protein ligase UBR1
Source.130: DFBPPR15279 ---- Microorganism protein ---- Nuclear fusion protein KAR5
Source.131: DFBPPR0003 ---- Plant protein ---- Conglutin beta 2
Source.132: DFBPPR0009 ---- Plant protein ---- Conglutin beta 1
Source.133: DFBPPR7793 ---- Plant protein ---- Photosystem II CP43 reaction center protein
Source.134: DFBPPR7821 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Source.135: DFBPPR7960 ---- Plant protein ---- Vicilin
Source.136: DFBPPR8034 ---- Plant protein ---- Linoleate 9S-lipoxygenase 1
Source.137: DFBPPR8059 ---- Plant protein ---- Photosystem II CP43 reaction center protein
Source.138: DFBPPR8237 ---- Plant protein ---- Photosystem II CP43 reaction center protein
Source.139: DFBPPR8283 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
Antioxidative activity

The peptide LLPH was designed based on an antioxidative peptide (Leu-Leu-Pro-His-His) derived from proteolytic digests of a soybean protein. It showed very weak antioxidative activity, as shown in Table 1.

Table 1. Synergistic Effects of Synthetic Peptides on the Antioxidative Activity of Nonpeptidic Antioxidants a

Antioxidative activity
peptide
+BHA
+BHT
+tocopherol
Antioxidant

1.0



BHA

2.4


BHT


1.9

tocopherol



1.3
Synthetic Peptide
HPLH0.613.1 (4.4)5.7 (2.3)7.2 (3.8)
HHLP0.916.1 (4.9)5.7 (2.0)9.4 (4.3)
HL0.97.4 (2.2)3.2 (1.1)3.9 (1.8)
HLPH1.29.3 (2.6)3.4 (1.1)3.9 (1.8)
LLPH
1.2
2.5 (0.7)1.4 (0.5)0.9 (0.4)
PLHH
1.6
10.3 (2.6)6.9 (2.0)10.1 (3.5)
HPHL
1.6
14.3 (3.6)6.2 (1.8)10.1 (3.5)
HH
2.3
13.6 (2.9)5.9 (1.4)8.2 (2.3)
LLHH
2.4
14.0 (2.9)6.5 (1.5)9.1 (2.5)
HHPLL
2.6
14.2 (2.8)5.7 (1.3)9.2 (2.4)
HLHP
2.7
11.9 (2.3)4.5 (1.0)6.4 (1.6)
LPHH
2.7
11.4 (2.2)6.2 (1.3)7.6 (1.9)
HHPL
2.9
14.2 (2.7)6.4 (1.3)8.1 (1.9)
LHPH
2.9
15.0 (2.8)6.3 (1.3)9.8 (2.3)
LH
3.0
12.1 (2.2)6.1 (1.2)9.2 (2.1)
LLPHH
3.0
9.3 (1.7)4.8 (1.0)3.1 (0.7)
HLH
3.2
14.0 (2.5)4.3 (0.8)6.5 (1.4)
LLPHHH
3.3
12.4 (2.2)5.8 (1.1)5.1 (1.1)
LHH
3.8
14.0 (2.3)6.3 (1.1)8.9 (1.7)
PHH
5.8
16.4 (2.0)6.6 (0.9)9.4 (1.3)
a Antioxidative activity was evaluated by the ferric thiocyanate method. The assay was performed with 40 μM peptides, 100 μM BHA and BHT, and 10 μM tocopherol. The number in parentheses is the magnitude of synergistic effect: (activity of peptide + antioxidant) / (activity of peptide) + (activity of antioxidant). Data represent the mean of three replications.
Specific target protein(s) N.D
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)O
Preparation method
Mode of preparation Chemical synthesis
Enzyme(s)/starter culture

Peptides were prepared by the fluorenylmethoxycarbonyl (Fmoc)-strategy using a simultaneous multiple peptide synthesizer (model PSSM-8, Shimadzu).

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
BIOPEP-UWM [D1] 3314
APD [D2] -
BioPepDB [D3] -
MBPDB [D4] -
Reference(s)
Primary literature Matoba N, Yamada Y, Yoshikawa M. Design of a genetically modified soybean protein preventing hypertension based on an anti-hypertensive peptide derived from ovalbumin. Curr Med Chem Cardiovasc Hematol Agents. 2003 Jun;1(2):197-202.
PMID: 15320699
Other literature(s)

[1] Tsuge N, Eikawa Y, Nomura Y, et al. Antioxidative Activity of Peptides Prepared by Enzymatic Hydrolysis of Egg-white Albumin.[J]. Nippon Nogeikagaku Kaishi, 1991, 65(11):1635-1641.
[2] Chen, H.M., et al., Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein. Journal of Agricultural and Food Chemistry, 1998. 46(1): p. 49-53.
[3] Chen H M, Muramoto K, Yamauchi F. Structural Analysis of Antioxidative Peptides from Soybean. beta.-Conglycinin[J]. J.agric.food Chem, 1995, 43(3):574-578.

PubDate 2003
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214