Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0023(Antioxidative peptide)

DFBP ID	DFBPANOX0023
Peptide sequence	LYPY
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Tyr-Pro-Tyr

Single-letter amino acid

LYPY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	554.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.87 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.1000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Designed peptide
Organism/Source	Designed peptide
Precursor protein	Designed peptide based on the antioxidative peptide: LLPHH
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1996 ---- Plant proteins ---- Transcription initiation factor IIB Source.2: DFBPPR2911 ---- Plant proteins ---- Probable V-type proton ATPase subunit d Source.3: DFBPPR4257 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL4 Source.4: DFBPPR4745 ---- Plant proteins ---- BURP domain-containing protein 9 Source.5: DFBPPR6008 ---- Plant proteins ---- Zein-beta Source.6: DFBPPR16190 ---- Animal proteins ---- Aprataxin Source.7: DFBPPR16479 ---- Animal proteins ---- Carboxypeptidase B Source.8: DFBPPR16581 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.9: DFBPPR16811 ---- Animal proteins ---- Carboxypeptidase A1 Source.10: DFBPPR18060 ---- Animal proteins ---- 2',5'-phosphodiesterase 12 Source.11: DFBPPR18088 ---- Animal proteins ---- Aprataxin Source.12: DFBPPR18276 ---- Animal proteins ---- 2-hydroxyacyl-CoA lyase 2 Source.13: DFBPPR18467 ---- Animal proteins ---- Alpha-aminoadipic semialdehyde synthase, mitochondrial Source.14: DFBPPR18843 ---- Animal proteins ---- Carboxypeptidase B Source.15: DFBPPR19738 ---- Animal proteins ---- Translocator protein Source.16: DFBPPR8992 ---- Animal proteins ---- Hyaluronidase-3 Source.17: DFBPPR9096 ---- Animal proteins ---- Carboxypeptidase A1 Source.18: DFBPPR9242 ---- Animal proteins ---- Carboxypeptidase B Source.19: DFBPPR9784 ---- Animal proteins ---- Translocator protein Source.20: DFBPPR10150 ---- Animal proteins ---- Tenascin Source.21: DFBPPR10579 ---- Animal proteins ---- Lysine-specific demethylase 5B Source.22: DFBPPR10897 ---- Animal proteins ---- Doublesex- and mab-3-related transcription factor 1 Source.23: DFBPPR11829 ---- Animal proteins ---- Melatonin receptor type 1B Source.24: DFBPPR13826 ---- Animal proteins ---- Translocator protein Source.25: DFBPPR8250 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide LYPY was designed based on an antioxidative peptide (Leu-Leu-Pro-His-His) derived from proteolytic digests of a soybean protein. It showed potent antioxidative activity (relative antioxidative activity: about 2.4, the peptide concentration was 4.0 × 10^-5 M, data represent the mean of three replications).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Chemical synthesis
Enzyme(s)/starter culture	Peptides were prepared by the fluorenylmethoxycarbonyl (Fmoc)-strategy using a simultaneous multiple peptide synthesizer (model PSSM-8, Shimadzu).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Matoba N, Yamada Y, Yoshikawa M. Design of a genetically modified soybean protein preventing hypertension based on an anti-hypertensive peptide derived from ovalbumin. Curr Med Chem Cardiovasc Hematol Agents. 2003 Jun;1(2):197-202. PMID: 15320699 DOI: 10.2174/1568016033477496
Other literature(s)	[1] Tsuge N, Eikawa Y, Nomura Y, et al. Antioxidative Activity of Peptides Prepared by Enzymatic Hydrolysis of Egg-white Albumin.[J]. Nippon Nogeikagaku Kaishi, 1991, 65(11):1635-1641. [2] Chen, H.M., et al., Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein. Journal of Agricultural and Food Chemistry, 1998. 46(1): p. 49-53. [3] Chen H M, Muramoto K, Yamauchi F. Structural Analysis of Antioxidative Peptides from Soybean. beta.-Conglycinin[J]. J.agric.food Chem, 1995, 43(3):574-578.
PubDate	2003