(1) The antioxidative activity of the peptide FD against the peroxidation of linoleic acid was determined in vitro, using the 1,3-diethyl-2-thiobarbituric acid method and the peptide showed 64.3% antioxidative activity. (2) The peptide FD can protect U3-1 cells against τ-BOOH-induced oxidative stress in vitro. The trypan blue dye exclusion assay was used to measure cell viability. Cell viability was significantly decreased in the control (39.2%) and in the sample treated with carnosine (41%) after τ-BOOH treatment. In contrast, when Phe-Asp was added to the medium, cell viability was significantly increased to 70.7%. |