Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0172(Antioxidative peptide)

DFBP ID	DFBPANOX0172
Peptide sequence	FGER
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Gly-Glu-Arg

Single-letter amino acid

FGER

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	507.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Potato
Precursor protein	Potato protein extract
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0928 ---- Plant proteins ---- Cytochrome P450 90B2 Source.2: DFBPPR0980 ---- Plant proteins ---- Ketol-acid reductoisomerase, chloroplastic Source.3: DFBPPR1160 ---- Plant proteins ---- Cytochrome P450 90A3 Source.4: DFBPPR1281 ---- Plant proteins ---- Arsenate reductase 2.2 Source.5: DFBPPR1368 ---- Plant proteins ---- Cytochrome P450 90A4 Source.6: DFBPPR1418 ---- Plant proteins ---- SCARECROW-LIKE protein 7 Source.7: DFBPPR1850 ---- Plant proteins ---- Replication factor C subunit 1 Source.8: DFBPPR2277 ---- Plant proteins ---- Protein TOC75, chloroplastic Source.9: DFBPPR2284 ---- Plant proteins ---- Probable glucan 1,3-alpha-glucosidase Source.10: DFBPPR2321 ---- Plant proteins ---- Aspartate carbamoyltransferase, chloroplastic Source.11: DFBPPR2379 ---- Plant proteins ---- Cysteine synthase Source.12: DFBPPR2418 ---- Plant proteins ---- CBL-interacting protein kinase 28 Source.13: DFBPPR2809 ---- Plant proteins ---- Retinoblastoma-related protein 1 Source.14: DFBPPR2862 ---- Plant proteins ---- Cysteine synthase Source.15: DFBPPR3366 ---- Plant proteins ---- Kinesin-like protein KIN-12C Source.16: DFBPPR3962 ---- Plant proteins ---- Myb-related protein MYBAS2 Source.17: DFBPPR4291 ---- Plant proteins ---- Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Source.18: DFBPPR4332 ---- Plant proteins ---- Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform Source.19: DFBPPR4515 ---- Plant proteins ---- 5'-adenylylsulfate reductase-like 6 Source.20: DFBPPR5609 ---- Plant proteins ---- Anthranilate O-methyltransferase 1 Source.21: DFBPPR5854 ---- Plant proteins ---- Retinoblastoma-related protein 3 Source.22: DFBPPR6063 ---- Plant proteins ---- Inactive anthranilate O-methyltransferase 1 Source.23: DFBPPR6210 ---- Plant proteins ---- Cysteine synthase Source.24: DFBPPR6226 ---- Plant proteins ---- Protein TOC75, chloroplastic Source.25: DFBPPR6283 ---- Plant proteins ---- Ketol-acid reductoisomerase, chloroplastic Source.26: DFBPPR6343 ---- Plant proteins ---- Aspartate carbamoyltransferase 3, chloroplastic Source.27: DFBPPR6344 ---- Plant proteins ---- Aspartate carbamoyltransferase 2, chloroplastic Source.28: DFBPPR6365 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.29: DFBPPR6366 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit beta, chloroplastic Source.30: DFBPPR6719 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.31: DFBPPR6756 ---- Plant proteins ---- Cysteine synthase Source.32: DFBPPR6867 ---- Plant proteins ---- Retinoblastoma-related protein 1 Source.33: DFBPPR7459 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit beta, chloroplastic Source.34: DFBPPR7460 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.35: DFBPPR7462 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.36: DFBPPR8463 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit beta, chloroplastic Source.37: DFBPPR16797 ---- Animal proteins ---- Albumin Source.38: DFBPPR16891 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.39: DFBPPR17283 ---- Animal proteins ---- NAD-dependent protein deacetylase sirtuin-7 Source.40: DFBPPR18907 ---- Animal proteins ---- Recombining binding protein suppressor of hairless Source.41: DFBPPR19964 ---- Animal proteins ---- MKI67 FHA domain-interacting nucleolar phosphoprotein Source.42: DFBPPR20255 ---- Animal proteins ---- Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2 Source.43: DFBPPR20383 ---- Animal proteins ---- Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial Source.44: DFBPPR21271 ---- Animal proteins ---- Selenocysteine lyase Source.45: DFBPPR21277 ---- Animal proteins ---- Glucose-6-phosphate exchanger SLC37A2 Source.46: DFBPPR22066 ---- Animal proteins ---- Pyridoxal phosphate homeostasis protein Source.47: DFBPPR8594 ---- Animal proteins ---- Trifunctional enzyme subunit alpha, mitochondrial Source.48: DFBPPR8885 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.49: DFBPPR9044 ---- Animal proteins ---- Albumin Source.50: DFBPPR9163 ---- Animal proteins ---- Cytochrome P450 11B1, mitochondrial Source.51: DFBPPR10201 ---- Animal proteins ---- Ephrin type-B receptor 1 Source.52: DFBPPR10446 ---- Animal proteins ---- Protein Wnt-8c Source.53: DFBPPR10729 ---- Animal proteins ---- Versican core protein Source.54: DFBPPR10964 ---- Animal proteins ---- Protein artemis Source.55: DFBPPR11542 ---- Animal proteins ---- Alpha-fetoprotein Source.56: DFBPPR12654 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.57: DFBPPR12746 ---- Animal proteins ---- Collagen alpha-4(IV) chain Source.58: DFBPPR12883 ---- Animal proteins ---- Cytochrome P450 2J1 Source.59: DFBPPR12981 ---- Animal proteins ---- Neutrophil antibiotic peptide NP-4 Source.60: DFBPPR13195 ---- Animal proteins ---- Albumin Source.61: DFBPPR13238 ---- Animal proteins ---- Laminin subunit gamma-2 Source.62: DFBPPR13241 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.63: DFBPPR13449 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.64: DFBPPR13655 ---- Animal proteins ---- Cholesterol side-chain cleavage enzyme, mitochondrial Source.65: DFBPPR13788 ---- Animal proteins ---- Albumin Source.66: DFBPPR14213 ---- Marine protein ---- Electron transfer flavoprotein regulatory factor 1 Source.67: DFBPPR14708 ---- Marine protein ---- Cytochrome P450 2K4 Source.68: DFBPPR14925 ---- Microorganism protein ---- 3-isopropylmalate dehydrogenase Source.69: DFBPPR15196 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP8
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide FGER showed potent antioxidative activity. The antioxidative activity was 32.1% and 77% by using ferric thiocyanate method and erythrocyte membrane ghost system, respectively. The peptide also inhibited ethanol-induced gastric damage with inhibition ratios of 67.9%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Two proteases: Protease 1 (Pancreatin) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and Protease 2 (Amano-P) (Amano Enzyme Inc., Nagoya, Japan).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide has shown real potent antioxidative activities and could further be investigated for potential use as food additives.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kudo, K., Onodera, S., Takeda, Y., Benkeblia, N., Shiomi, N. Antioxidative activities of some peptides isolated from hydrolyzed potato protein extract. Journal of Functional Foods. 2009, 1, 170-6. DOI: 10.1016/j.jff.2009.01.006
Other literature(s)	No data
PubDate	2009