Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0173(Antioxidative peptide)

DFBP ID	DFBPANOX0173
Peptide sequence	FDRR
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Asp-Arg-Arg

Single-letter amino acid

FDRR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	592.64 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.88 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.4250^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Potato
Precursor protein	Potato protein extract
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0875 ---- Plant proteins ---- ABC transporter G family member 5 Source.2: DFBPPR1362 ---- Plant proteins ---- Protein ROS1C Source.3: DFBPPR1552 ---- Plant proteins ---- Phosphatidylserine decarboxylase proenzyme 2 Source.4: DFBPPR1587 ---- Plant proteins ---- CBL-interacting protein kinase 8 Source.5: DFBPPR1632 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 6, chloroplastic Source.6: DFBPPR1844 ---- Plant proteins ---- Heat shock 70 kDa protein BIP2 Source.7: DFBPPR1936 ---- Plant proteins ---- tRNA wybutosine-synthesizing protein 2/3/4 Source.8: DFBPPR2896 ---- Plant proteins ---- Kinesin-like protein KIN-7E, chloroplastic Source.9: DFBPPR3128 ---- Plant proteins ---- ABC transporter G family member 41 Source.10: DFBPPR3438 ---- Plant proteins ---- Protein ROLLING AND ERECT LEAF 2 Source.11: DFBPPR5118 ---- Plant proteins ---- Trans-cinnamate 4-monooxygenase Source.12: DFBPPR5454 ---- Plant proteins ---- Ferredoxin-dependent glutamate synthase, chloroplastic Source.13: DFBPPR5778 ---- Plant proteins ---- DNA mismatch repair protein MSH2 Source.14: DFBPPR5788 ---- Plant proteins ---- Globulin-1 S allele Source.15: DFBPPR6415 ---- Plant proteins ---- Trans-cinnamate 4-monooxygenase Source.16: DFBPPR6725 ---- Plant proteins ---- Granule-bound starch synthase 1, chloroplastic/amyloplastic Source.17: DFBPPR8430 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.18: DFBPPR15983 ---- Animal proteins ---- Tight junction protein ZO-1 Source.19: DFBPPR16490 ---- Animal proteins ---- Translocon-associated protein subunit beta Source.20: DFBPPR17126 ---- Animal proteins ---- cAMP-dependent protein kinase type II-alpha regulatory subunit Source.21: DFBPPR17353 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase-like N Source.22: DFBPPR17550 ---- Animal proteins ---- Serine/threonine-protein kinase PLK4 Source.23: DFBPPR17604 ---- Animal proteins ---- Regulator of telomere elongation helicase 1 Source.24: DFBPPR17802 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP4 Source.25: DFBPPR18483 ---- Animal proteins ---- DNA damage-binding protein 2 Source.26: DFBPPR18886 ---- Animal proteins ---- Interleukin-12 receptor subunit beta-2 Source.27: DFBPPR20168 ---- Animal proteins ---- Alpha-actinin-2 Source.28: DFBPPR20476 ---- Animal proteins ---- Translocon-associated protein subunit beta Source.29: DFBPPR22141 ---- Animal proteins ---- Tumor necrosis factor alpha-induced protein 8-like protein 1 Source.30: DFBPPR22225 ---- Animal proteins ---- F-box/WD repeat-containing protein 9 Source.31: DFBPPR22543 ---- Animal proteins ---- Haloacid dehalogenase-like hydrolase domain-containing protein 3 Source.32: DFBPPR8763 ---- Animal proteins ---- cAMP-dependent protein kinase type II-alpha regulatory subunit Source.33: DFBPPR10609 ---- Animal proteins ---- Homeobox protein DLX-5 Source.34: DFBPPR10697 ---- Animal proteins ---- Alpha-actinin-2 Source.35: DFBPPR10955 ---- Animal proteins ---- DNA damage-binding protein 2 Source.36: DFBPPR11705 ---- Animal proteins ---- SCO-spondin Source.37: DFBPPR12213 ---- Animal proteins ---- Tumor necrosis factor alpha-induced protein 8-like protein 1 Source.38: DFBPPR12295 ---- Animal proteins ---- Sodium/hydrogen exchanger 3 Source.39: DFBPPR12356 ---- Animal proteins ---- Very low-density lipoprotein receptor Source.40: DFBPPR13708 ---- Animal proteins ---- Renin Source.41: DFBPPR14271 ---- Marine protein ---- Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Source.42: DFBPPR15137 ---- Microorganism protein ---- Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase Source.43: DFBPPR15539 ---- Microorganism protein ---- Acyl-coenzyme A oxidase Source.44: DFBPPR8080 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic Source.45: DFBPPR8250 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide FDRR showed potent antioxidative activity. The antioxidative activity was 93.0% and 83% by using ferric thiocyanate method and erythrocyte membrane ghost system, respectively. The peptide also inhibited ethanol-induced gastric damage with inhibition ratios of 57.0%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Two proteases: Protease 1 (Pancreatin) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and Protease 2 (Amano-P) (Amano Enzyme Inc., Nagoya, Japan).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide has shown real potent antioxidative activities and could further be investigated for potential use as food additives.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kudo, K., Onodera, S., Takeda, Y., Benkeblia, N., Shiomi, N. Antioxidative activities of some peptides isolated from hydrolyzed potato protein extract. Journal of Functional Foods. 2009, 1, 170-6. DOI: 10.1016/j.jff.2009.01.006
Other literature(s)	No data
PubDate	2009