Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0174(Antioxidative peptide)

DFBP ID	DFBPANOX0174
Peptide sequence	FGERR
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Gly-Glu-Arg-Arg

Single-letter amino acid

FGERR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	663.73 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.88 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.0200^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Potato
Precursor protein	Potato protein extract
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2284 ---- Plant proteins ---- Probable glucan 1,3-alpha-glucosidase Source.2: DFBPPR3962 ---- Plant proteins ---- Myb-related protein MYBAS2 Source.3: DFBPPR6365 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.4: DFBPPR6719 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.5: DFBPPR7460 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.6: DFBPPR7462 ---- Plant proteins ---- RuBisCO large subunit-binding protein subunit alpha, chloroplastic Source.7: DFBPPR20383 ---- Animal proteins ---- Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial Source.8: DFBPPR15196 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP8
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide FGERR showed potent antioxidative activity. The antioxidative activity was 93.4% and 72% by using ferric thiocyanate method and erythrocyte membrane ghost system, respectively. The peptide also inhibited ethanol-induced gastric damage with inhibition ratios of 60.3%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Two proteases: Protease 1 (Pancreatin) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and Protease 2 (Amano-P) (Amano Enzyme Inc., Nagoya, Japan).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide has shown real potent antioxidative activities and could further be investigated for potential use as food additives.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kudo, K., Onodera, S., Takeda, Y., Benkeblia, N., Shiomi, N. Antioxidative activities of some peptides isolated from hydrolyzed potato protein extract. Journal of Functional Foods. 2009, 1, 170-6. DOI: 10.1016/j.jff.2009.01.006
Other literature(s)	No data
PubDate	2009