Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0175(Antioxidative peptide)

DFBP ID	DFBPANOX0175
Peptide sequence	NHAV
Type	Native peptide
Peptide/Function name	antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asn-His-Ala-Val

Single-letter amino acid

NHAV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
441.02 Da	439.47 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.1750^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hempseed
Precursor protein	Hempseed protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2914 ---- Plant proteins ---- Glutelin type-D 1 Source.2: DFBPPR3171 ---- Plant proteins ---- Putrescine hydroxycinnamoyltransferase Source.3: DFBPPR3185 ---- Plant proteins ---- Acyl transferase 10 Source.4: DFBPPR3227 ---- Plant proteins ---- Oryzain gamma chain Source.5: DFBPPR3327 ---- Plant proteins ---- Putative mannan endo-1,4-beta-mannosidase 9 Source.6: DFBPPR3533 ---- Plant proteins ---- Peroxisomal membrane protein 11-4 Source.7: DFBPPR3703 ---- Plant proteins ---- Zinc-finger homeodomain protein 1 Source.8: DFBPPR3704 ---- Plant proteins ---- Zinc-finger homeodomain protein 2 Source.9: DFBPPR4648 ---- Plant proteins ---- SPX domain-containing membrane protein Os04g0573000 Source.10: DFBPPR6208 ---- Plant proteins ---- Cysteine proteinase 2 Source.11: DFBPPR6345 ---- Plant proteins ---- Aspartate carbamoyltransferase 1, chloroplastic Source.12: DFBPPR7204 ---- Plant proteins ---- Thiol protease aleurain Source.13: DFBPPR7596 ---- Milk proteins ---- Lactotransferrin Source.14: DFBPPR7658 ---- Milk proteins ---- Lactotransferrin Source.15: DFBPPR7669 ---- Milk proteins ---- Lactotransferrin Source.16: DFBPPR7683 ---- Milk proteins ---- Lactotransferrin Source.17: DFBPPR7713 ---- Milk proteins ---- Lactotransferrin Source.18: DFBPPR8500 ---- Milk proteins ---- Lactotransferrin Source.19: DFBPPR16262 ---- Animal proteins ---- Dipeptidyl peptidase 1 Source.20: DFBPPR16304 ---- Animal proteins ---- Cathepsin K Source.21: DFBPPR16332 ---- Animal proteins ---- Transcription factor 4 Source.22: DFBPPR16333 ---- Animal proteins ---- Transcription factor 4 Source.23: DFBPPR16721 ---- Animal proteins ---- Testin Source.24: DFBPPR17364 ---- Animal proteins ---- Pro-cathepsin H Source.25: DFBPPR17921 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.26: DFBPPR18368 ---- Animal proteins ---- E3 ubiquitin-protein ligase TRIP12 Source.27: DFBPPR18929 ---- Animal proteins ---- Dipeptidyl peptidase 1 Source.28: DFBPPR18952 ---- Animal proteins ---- Serotransferrin Source.29: DFBPPR19201 ---- Animal proteins ---- 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial Source.30: DFBPPR19746 ---- Animal proteins ---- tRNA pseudouridine(38/39) synthase Source.31: DFBPPR21044 ---- Animal proteins ---- Conserved oligomeric Golgi complex subunit 7 Source.32: DFBPPR21763 ---- Animal proteins ---- Protein GOLM2 Source.33: DFBPPR22591 ---- Animal proteins ---- Leucine-rich repeat-containing protein 51 Source.34: DFBPPR8627 ---- Animal proteins ---- UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Source.35: DFBPPR8735 ---- Animal proteins ---- Pro-cathepsin H Source.36: DFBPPR8838 ---- Animal proteins ---- Cathepsin K Source.37: DFBPPR9015 ---- Animal proteins ---- Serotransferrin Source.38: DFBPPR9125 ---- Animal proteins ---- G protein-activated inward rectifier potassium channel 4 Source.39: DFBPPR9814 ---- Animal proteins ---- Testin Source.40: DFBPPR10860 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.41: DFBPPR10909 ---- Animal proteins ---- Transcription factor 12 Source.42: DFBPPR10930 ---- Animal proteins ---- WD repeat-containing protein 48 Source.43: DFBPPR11660 ---- Animal proteins ---- Cathepsin K Source.44: DFBPPR12251 ---- Animal proteins ---- Serotransferrin Source.45: DFBPPR12302 ---- Animal proteins ---- UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Source.46: DFBPPR12460 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, platelet type Source.47: DFBPPR12507 ---- Animal proteins ---- Cathepsin K Source.48: DFBPPR12882 ---- Animal proteins ---- Parathyroid hormone-related protein Source.49: DFBPPR13092 ---- Animal proteins ---- Testin Source.50: DFBPPR13263 ---- Animal proteins ---- Serotransferrin Source.51: DFBPPR13409 ---- Animal proteins ---- Testin Source.52: DFBPPR13918 ---- Animal proteins ---- Testin Source.53: DFBPPR14110 ---- Marine protein ---- BRCA1-A complex subunit Abraxas 1 Source.54: DFBPPR14924 ---- Microorganism protein ---- E3 ubiquitin-protein ligase UBR1 Source.55: DFBPPR15252 ---- Microorganism protein ---- Protein DSE2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The protective effects of the purified peptides on hydrogen peroxide-induced cell apoptosis were investigated in rat pheochromocytoma line PC12 cells. The peptide NHAV, at a concentration of 10 lg/ml, possessed protective effects (P < 0.05) against cell death and oxidative apoptosis.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolyzed with Alcalase at 9.4 pH and 50 ℃ for 1 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The findings are significant for the discovery and development of natural antioxidants from hempseed by-products.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Lu, R.-R., Qian, P., Sun, Z., Zhou, X.-H., Chen, T.-P., He, J.-F., et al. Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells. Food Chem. 2010, 123, 1210-8. DOI: 10.1016/j.foodchem.2010.05.089
Other literature(s)	No data
PubDate	2010