Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0302(Antioxidative peptide)

DFBP ID	DFBPANOX0302
Peptide sequence	PAGY
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Ala-Gly-Tyr

Single-letter amino acid

PAGY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
457 Da	406.43 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.3750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Fish, Marine
Organism/Source	Tuna
Precursor protein	Tuna cooking juice
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0829 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC5 Source.2: DFBPPR0830 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC6 Source.3: DFBPPR0964 ---- Plant proteins ---- Thioredoxin reductase NTRC Source.4: DFBPPR1424 ---- Plant proteins ---- Germin-like protein 8-14 Source.5: DFBPPR3171 ---- Plant proteins ---- Putrescine hydroxycinnamoyltransferase Source.6: DFBPPR3390 ---- Plant proteins ---- Probable nucleoredoxin 1-2 Source.7: DFBPPR3703 ---- Plant proteins ---- Zinc-finger homeodomain protein 1 Source.8: DFBPPR3704 ---- Plant proteins ---- Zinc-finger homeodomain protein 2 Source.9: DFBPPR3953 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 16 Source.10: DFBPPR4762 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 35 Source.11: DFBPPR4990 ---- Plant proteins ---- Beta-conglycinin alpha' subunit Source.12: DFBPPR5025 ---- Plant proteins ---- Beta-conglycinin alpha subunit 1 Source.13: DFBPPR5036 ---- Plant proteins ---- Beta-conglycinin alpha subunit 2 Source.14: DFBPPR5570 ---- Plant proteins ---- ATP synthase subunit beta, mitochondrial Source.15: DFBPPR6396 ---- Plant proteins ---- Hydroxyproline O-arabinosyltransferase NOD3 Source.16: DFBPPR7469 ---- Plant proteins ---- Germin-like protein 1 Source.17: DFBPPR16131 ---- Animal proteins ---- Pyruvate kinase PKLR Source.18: DFBPPR16224 ---- Animal proteins ---- Uromodulin Source.19: DFBPPR16646 ---- Animal proteins ---- AT-rich interactive domain-containing protein 5B Source.20: DFBPPR17228 ---- Animal proteins ---- Lanosterol synthase Source.21: DFBPPR17576 ---- Animal proteins ---- Fibrillin-1 Source.22: DFBPPR17626 ---- Animal proteins ---- Elastin Source.23: DFBPPR18072 ---- Animal proteins ---- Postacrosomal sheath WW domain-binding protein Source.24: DFBPPR18402 ---- Animal proteins ---- Uromodulin Source.25: DFBPPR18511 ---- Animal proteins ---- Complement C1s subcomponent Source.26: DFBPPR18737 ---- Animal proteins ---- Centrosomal protein of 120 kDa Source.27: DFBPPR18794 ---- Animal proteins ---- LIM domain-containing protein ajuba Source.28: DFBPPR18908 ---- Animal proteins ---- Proteasome subunit alpha type-6 Source.29: DFBPPR19600 ---- Animal proteins ---- Macrophage-expressed gene 1 protein Source.30: DFBPPR20060 ---- Animal proteins ---- Fibulin-5 Source.31: DFBPPR20142 ---- Animal proteins ---- Kinesin-like protein KIF3C Source.32: DFBPPR20486 ---- Animal proteins ---- Ethanolamine-phosphate phospho-lyase Source.33: DFBPPR20674 ---- Animal proteins ---- Stromal membrane-associated protein 2 Source.34: DFBPPR20914 ---- Animal proteins ---- AT-rich interactive domain-containing protein 5B Source.35: DFBPPR21101 ---- Animal proteins ---- Bromodomain-containing protein 2 Source.36: DFBPPR21111 ---- Animal proteins ---- Zinc finger protein-like 1 Source.37: DFBPPR21125 ---- Animal proteins ---- Male-enhanced antigen 1 Source.38: DFBPPR21261 ---- Animal proteins ---- tRNA selenocysteine 1-associated protein 1 Source.39: DFBPPR21492 ---- Animal proteins ---- Homeobox protein DBX1 Source.40: DFBPPR22053 ---- Animal proteins ---- Protein FAM118B Source.41: DFBPPR8591 ---- Animal proteins ---- Glutathione hydrolase 1 proenzyme Source.42: DFBPPR8923 ---- Animal proteins ---- Cubilin Source.43: DFBPPR9102 ---- Animal proteins ---- Nuclear factor of activated T-cells, cytoplasmic 1 Source.44: DFBPPR9865 ---- Animal proteins ---- Male-enhanced antigen 1 Source.45: DFBPPR10218 ---- Animal proteins ---- Occludin Source.46: DFBPPR10501 ---- Animal proteins ---- Very low-density lipoprotein receptor Source.47: DFBPPR10520 ---- Animal proteins ---- Collagen alpha-2(IX) chain Source.48: DFBPPR10668 ---- Animal proteins ---- E3 UFM1-protein ligase 1 Source.49: DFBPPR10735 ---- Animal proteins ---- Semaphorin-3E Source.50: DFBPPR10953 ---- Animal proteins ---- DNA repair and recombination protein RAD54-like Source.51: DFBPPR11314 ---- Animal proteins ---- Multivesicular body subunit 12A Source.52: DFBPPR11771 ---- Animal proteins ---- Homeobox protein goosecoid Source.53: DFBPPR11963 ---- Animal proteins ---- Stromal membrane-associated protein 2 Source.54: DFBPPR11996 ---- Animal proteins ---- Homeobox protein Hox-A6 Source.55: DFBPPR12310 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1C Source.56: DFBPPR12422 ---- Animal proteins ---- Voltage-dependent R-type calcium channel subunit alpha-1E Source.57: DFBPPR12453 ---- Animal proteins ---- Lactase-phlorizin hydrolase Source.58: DFBPPR12835 ---- Animal proteins ---- Chloride channel protein ClC-Ka Source.59: DFBPPR15876 ---- Microorganism protein ---- Probable DNA-directed RNA polymerase Source.60: DFBPPR0003 ---- Plant protein ---- Conglutin beta 2 Source.61: DFBPPR0009 ---- Plant protein ---- Conglutin beta 1 Source.62: DFBPPR7960 ---- Plant protein ---- Vicilin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide showed potent 1,1-diphenyl-2-picrylhydrzyl (DPPH) scavenging activity with a scavenging effect of 75% at concentration of 87 μg/mL.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Protease XXIII, from Aspergillus oryzae, was used to hydrolyze tuna cooking juice at 37 ℃ for up to 6 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Hydrolyzates from tuna cooking juice, and the fractions derived from them, can be incorporated into lipid-containing foods as chain-breaking antioxidants to minimize free radical-mediated lipid peroxidation. These hydrolyzates can also be used as alternatives to conventional drugs for treating human diseases associated with free radical-mediated tissue damage.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Jao, C.-L., Ko, W.-C. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging by protein hydrolyzates from tuna cooking juice. Fisheries Science. 2002, 68, 430-5. DOI: 10.1046/j.1444-2906.2002.00442.x
Other literature(s)	N.D
PubDate	2002