Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0413(Antioxidative peptide)

DFBP ID	DFBPANOX0413
Peptide sequence	LVSK
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Val-Ser-Lys

Single-letter amino acid

LVSK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
446.27 Da	445.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.8250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Marine bivalve (Mactra veneriformis)
Precursor protein	Marine bivalve hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0927 ---- Plant proteins ---- Serine/threonine-protein kinase TOR Source.2: DFBPPR1065 ---- Plant proteins ---- Protein TIFY 3 Source.3: DFBPPR1156 ---- Plant proteins ---- Calcium-dependent protein kinase 19 Source.4: DFBPPR1165 ---- Plant proteins ---- Chaperone protein dnaJ A7A, chloroplastic Source.5: DFBPPR1178 ---- Plant proteins ---- Chaperone protein dnaJ A7B, chloroplastic Source.6: DFBPPR1318 ---- Plant proteins ---- Calcium-dependent protein kinase 22 Source.7: DFBPPR1336 ---- Plant proteins ---- Protein argonaute 7 Source.8: DFBPPR1530 ---- Plant proteins ---- Ent-kaur-16-ene synthase, chloroplastic Source.9: DFBPPR1607 ---- Plant proteins ---- Arginine biosynthesis bifunctional protein ArgJ, chloroplastic Source.10: DFBPPR1713 ---- Plant proteins ---- Poly [ADP-ribose] polymerase 1 Source.11: DFBPPR1784 ---- Plant proteins ---- Pentatricopeptide repeat-containing protein OTP51, chloroplastic Source.12: DFBPPR1839 ---- Plant proteins ---- Laccase-24 Source.13: DFBPPR1877 ---- Plant proteins ---- Cyclin-dependent kinase F-4 Source.14: DFBPPR1902 ---- Plant proteins ---- Transcription factor ILI6 Source.15: DFBPPR1947 ---- Plant proteins ---- Calcium-transporting ATPase 10, plasma membrane-type Source.16: DFBPPR2129 ---- Plant proteins ---- Kinesin-like protein KIN-5C Source.17: DFBPPR2191 ---- Plant proteins ---- Ent-pimara-8(14),15-diene synthase Source.18: DFBPPR2209 ---- Plant proteins ---- Succinate dehydrogenase subunit 4, mitochondrial Source.19: DFBPPR2442 ---- Plant proteins ---- Vacuolar cation/proton exchanger 1c Source.20: DFBPPR2674 ---- Plant proteins ---- Probable inorganic phosphate transporter 1-3 Source.21: DFBPPR3011 ---- Plant proteins ---- Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG4 Source.22: DFBPPR3070 ---- Plant proteins ---- Putative D-cysteine desulfhydrase 1, mitochondrial Source.23: DFBPPR3121 ---- Plant proteins ---- Endoglucanase 23 Source.24: DFBPPR3562 ---- Plant proteins ---- Probable protein phosphatase 2C 61 Source.25: DFBPPR3737 ---- Plant proteins ---- Probable protein phosphatase 2C 28 Source.26: DFBPPR3777 ---- Plant proteins ---- Probable protein phosphatase 2C 38 Source.27: DFBPPR3803 ---- Plant proteins ---- Probable trehalase Source.28: DFBPPR3883 ---- Plant proteins ---- Cyclin-D5-1 Source.29: DFBPPR3966 ---- Plant proteins ---- Probable trehalose-phosphate phosphatase 7 Source.30: DFBPPR4051 ---- Plant proteins ---- 40S ribosomal protein S3a Source.31: DFBPPR4071 ---- Plant proteins ---- Probable trehalose-phosphate phosphatase 6 Source.32: DFBPPR4085 ---- Plant proteins ---- Probable trehalose-phosphate phosphatase 8 Source.33: DFBPPR4278 ---- Plant proteins ---- Calcium-binding protein CBP Source.34: DFBPPR4289 ---- Plant proteins ---- Probable trehalose-phosphate phosphatase 4 Source.35: DFBPPR4343 ---- Plant proteins ---- Transcription factor ILI7 Source.36: DFBPPR4347 ---- Plant proteins ---- Metal transporter Nramp2 Source.37: DFBPPR4374 ---- Plant proteins ---- WUSCHEL-related homeobox 10 Source.38: DFBPPR4479 ---- Plant proteins ---- Metal transporter Nramp6 Source.39: DFBPPR4494 ---- Plant proteins ---- CRS2-associated factor 1, mitochondrial Source.40: DFBPPR4534 ---- Plant proteins ---- Protein argonaute 4A Source.41: DFBPPR4661 ---- Plant proteins ---- Protein argonaute 4B Source.42: DFBPPR4900 ---- Plant proteins ---- Histone-lysine N-methyltransferase CLF Source.43: DFBPPR4934 ---- Plant proteins ---- U-box domain-containing protein 70 Source.44: DFBPPR4941 ---- Plant proteins ---- Chaperone protein dnaJ A8, chloroplastic Source.45: DFBPPR5059 ---- Plant proteins ---- Phosphoenolpyruvate carboxylase Source.46: DFBPPR5235 ---- Plant proteins ---- DNA-directed RNA polymerase II subunit RPB7 Source.47: DFBPPR5304 ---- Plant proteins ---- Maturase K Source.48: DFBPPR5400 ---- Plant proteins ---- Indole-3-acetaldehyde oxidase Source.49: DFBPPR5524 ---- Plant proteins ---- Calcium-dependent protein kinase 2 Source.50: DFBPPR5579 ---- Plant proteins ---- Indole-3-acetaldehyde oxidase Source.51: DFBPPR5598 ---- Plant proteins ---- Trehalose 6-phosphate phosphatase RA3 Source.52: DFBPPR5604 ---- Plant proteins ---- Poly [ADP-ribose] polymerase 1 Source.53: DFBPPR5663 ---- Plant proteins ---- Arginine biosynthesis bifunctional protein ArgJ, chloroplastic Source.54: DFBPPR5742 ---- Plant proteins ---- Methylthioribose-1-phosphate isomerase Source.55: DFBPPR6450 ---- Plant proteins ---- Chalcone synthase 1A Source.56: DFBPPR7659 ---- Milk proteins ---- Glycosylation-dependent cell adhesion molecule 1 Source.57: DFBPPR15983 ---- Animal proteins ---- Tight junction protein ZO-1 Source.58: DFBPPR16203 ---- Animal proteins ---- E3 ubiquitin-protein ligase RING1 Source.59: DFBPPR16243 ---- Animal proteins ---- Retinoic acid receptor RXR-beta Source.60: DFBPPR16279 ---- Animal proteins ---- Galactokinase Source.61: DFBPPR16559 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.62: DFBPPR16563 ---- Animal proteins ---- Myosin-16 Source.63: DFBPPR16567 ---- Animal proteins ---- Oxygen-regulated protein 1 Source.64: DFBPPR16647 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.65: DFBPPR16684 ---- Animal proteins ---- UDP-N-acetylglucosamine transporter Source.66: DFBPPR16811 ---- Animal proteins ---- Carboxypeptidase A1 Source.67: DFBPPR16832 ---- Animal proteins ---- ATP synthase subunit alpha, mitochondrial Source.68: DFBPPR16866 ---- Animal proteins ---- Endothelin receptor type B Source.69: DFBPPR17264 ---- Animal proteins ---- Tubulin-specific chaperone D Source.70: DFBPPR17267 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 4B Source.71: DFBPPR17508 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPD Source.72: DFBPPR17519 ---- Animal proteins ---- Alpha-enolase Source.73: DFBPPR17739 ---- Animal proteins ---- Molybdenum cofactor biosynthesis protein 1 Source.74: DFBPPR17810 ---- Animal proteins ---- Histone H1.2 Source.75: DFBPPR18085 ---- Animal proteins ---- Staphylococcal nuclease domain-containing protein 1 Source.76: DFBPPR18136 ---- Animal proteins ---- Septin-8 Source.77: DFBPPR18137 ---- Animal proteins ---- Septin-8 Source.78: DFBPPR18245 ---- Animal proteins ---- ATP synthase subunit a Source.79: DFBPPR18275 ---- Animal proteins ---- Enoyl-CoA hydratase, mitochondrial Source.80: DFBPPR18323 ---- Animal proteins ---- Transcription factor E2F7 Source.81: DFBPPR18330 ---- Animal proteins ---- Retinoic acid receptor RXR-gamma Source.82: DFBPPR18332 ---- Animal proteins ---- G protein-activated inward rectifier potassium channel 1 Source.83: DFBPPR18497 ---- Animal proteins ---- Synaptobrevin homolog YKT6 Source.84: DFBPPR18550 ---- Animal proteins ---- Prostaglandin E2 receptor EP3 subtype Source.85: DFBPPR18620 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.86: DFBPPR18628 ---- Animal proteins ---- Cytochrome b5 reductase 4 Source.87: DFBPPR18646 ---- Animal proteins ---- Angiopoietin-2 Source.88: DFBPPR18946 ---- Animal proteins ---- Paternally-expressed gene 3 protein Source.89: DFBPPR19004 ---- Animal proteins ---- Histone H1.3 Source.90: DFBPPR19226 ---- Animal proteins ---- Caseinolytic peptidase B protein homolog Source.91: DFBPPR19506 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.92: DFBPPR19569 ---- Animal proteins ---- Natural resistance-associated macrophage protein 1 Source.93: DFBPPR19587 ---- Animal proteins ---- Volume-regulated anion channel subunit LRRC8C Source.94: DFBPPR19669 ---- Animal proteins ---- Cullin-associated NEDD8-dissociated protein 1 Source.95: DFBPPR19709 ---- Animal proteins ---- Centromere protein X Source.96: DFBPPR19980 ---- Animal proteins ---- Exocyst complex component 3 Source.97: DFBPPR20057 ---- Animal proteins ---- AP-3 complex subunit sigma-1 Source.98: DFBPPR20142 ---- Animal proteins ---- Kinesin-like protein KIF3C Source.99: DFBPPR20322 ---- Animal proteins ---- GTPase Era, mitochondrial Source.100: DFBPPR20355 ---- Animal proteins ---- RING finger protein 10 Source.101: DFBPPR20385 ---- Animal proteins ---- UDP-N-acetylglucosamine transporter Source.102: DFBPPR20405 ---- Animal proteins ---- Oxygen-regulated protein 1 Source.103: DFBPPR20419 ---- Animal proteins ---- Solute carrier family 52, riboflavin transporter, member 3 Source.104: DFBPPR20616 ---- Animal proteins ---- Zinc transporter ZIP13 Source.105: DFBPPR20618 ---- Animal proteins ---- Large subunit GTPase 1 homolog Source.106: DFBPPR20840 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 3 Source.107: DFBPPR20878 ---- Animal proteins ---- Protein SMG9 Source.108: DFBPPR20899 ---- Animal proteins ---- Centrosomal protein kizuna Source.109: DFBPPR20968 ---- Animal proteins ---- Ras GTPase-activating protein 3 Source.110: DFBPPR20981 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.111: DFBPPR21018 ---- Animal proteins ---- Solute carrier family 22 member 17 Source.112: DFBPPR21534 ---- Animal proteins ---- 39S ribosomal protein L46, mitochondrial Source.113: DFBPPR21784 ---- Animal proteins ---- O(6)-methylguanine-induced apoptosis 2 Source.114: DFBPPR21887 ---- Animal proteins ---- Cyclin-G1 Source.115: DFBPPR22094 ---- Animal proteins ---- Protein MMS22-like Source.116: DFBPPR22131 ---- Animal proteins ---- F-box/WD repeat-containing protein 2 Source.117: DFBPPR22182 ---- Animal proteins ---- Protein TOPAZ1 Source.118: DFBPPR22344 ---- Animal proteins ---- Divergent protein kinase domain 2B Source.119: DFBPPR22382 ---- Animal proteins ---- 40S ribosomal protein S25 Source.120: DFBPPR22642 ---- Animal proteins ---- BTB/POZ domain-containing protein KCTD4 Source.121: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.122: DFBPPR8886 ---- Animal proteins ---- Endothelin receptor type B Source.123: DFBPPR9014 ---- Animal proteins ---- ATP synthase subunit alpha, mitochondrial Source.124: DFBPPR9075 ---- Animal proteins ---- Retinoic acid receptor RXR-gamma Source.125: DFBPPR9096 ---- Animal proteins ---- Carboxypeptidase A1 Source.126: DFBPPR9165 ---- Animal proteins ---- Solute carrier family 22 member 2 Source.127: DFBPPR9342 ---- Animal proteins ---- Proteasome activator complex subunit 3 Source.128: DFBPPR9504 ---- Animal proteins ---- Angiopoietin-2 Source.129: DFBPPR9610 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.130: DFBPPR9625 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.131: DFBPPR9648 ---- Animal proteins ---- Secretogranin-2 Source.132: DFBPPR9903 ---- Animal proteins ---- Cyclin-G1 Source.133: DFBPPR10146 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit beta-3 Source.134: DFBPPR10232 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-4 Source.135: DFBPPR10334 ---- Animal proteins ---- Retinoic acid receptor RXR-gamma Source.136: DFBPPR10542 ---- Animal proteins ---- Erythroid transcription factor Source.137: DFBPPR10603 ---- Animal proteins ---- Collagen alpha-3(VI) chain Source.138: DFBPPR10696 ---- Animal proteins ---- pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 Source.139: DFBPPR10738 ---- Animal proteins ---- Histone H1.10 Source.140: DFBPPR10790 ---- Animal proteins ---- Histone H1 Source.141: DFBPPR10822 ---- Animal proteins ---- Ribosomal protein S6 kinase 2 alpha Source.142: DFBPPR10877 ---- Animal proteins ---- Histone H1.01 Source.143: DFBPPR10932 ---- Animal proteins ---- Histone H1.11L Source.144: DFBPPR10947 ---- Animal proteins ---- Cytosolic carboxypeptidase 1 Source.145: DFBPPR10997 ---- Animal proteins ---- Tubulin-specific chaperone D Source.146: DFBPPR11107 ---- Animal proteins ---- Zinc finger protein GLI1 Source.147: DFBPPR11249 ---- Animal proteins ---- Asparagine synthetase [glutamine-hydrolyzing] Source.148: DFBPPR11302 ---- Animal proteins ---- Histone H1.11R Source.149: DFBPPR11308 ---- Animal proteins ---- Histone H1.03 Source.150: DFBPPR11330 ---- Animal proteins ---- Proteasome activator complex subunit 3 Source.151: DFBPPR11334 ---- Animal proteins ---- Heme oxygenase 1 Source.152: DFBPPR11337 ---- Animal proteins ---- G protein-activated inward rectifier potassium channel 1 Source.153: DFBPPR11446 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 48 Source.154: DFBPPR11588 ---- Animal proteins ---- Acetylserotonin O-methyltransferase Source.155: DFBPPR11626 ---- Animal proteins ---- tRNA-splicing endonuclease subunit Sen2 Source.156: DFBPPR11760 ---- Animal proteins ---- Cysteine-rich motor neuron 1 protein Source.157: DFBPPR12290 ---- Animal proteins ---- Ryanodine receptor 2 Source.158: DFBPPR12693 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.159: DFBPPR12729 ---- Animal proteins ---- Solute carrier family 22 member 2 Source.160: DFBPPR12738 ---- Animal proteins ---- Histone H1.4 Source.161: DFBPPR12820 ---- Animal proteins ---- Endothelin receptor type B Source.162: DFBPPR12920 ---- Animal proteins ---- Arylamine N-acetyltransferase 2 Source.163: DFBPPR12935 ---- Animal proteins ---- Histone H1.3 Source.164: DFBPPR12957 ---- Animal proteins ---- Arylamine N-acetyltransferase 1 Source.165: DFBPPR13164 ---- Animal proteins ---- Cystic fibrosis transmembrane conductance regulator Source.166: DFBPPR13244 ---- Animal proteins ---- Endothelin receptor type B Source.167: DFBPPR13825 ---- Animal proteins ---- ATP synthase subunit a Source.168: DFBPPR13891 ---- Animal proteins ---- Natural resistance-associated macrophage protein 1 Source.169: DFBPPR13950 ---- Animal proteins ---- Protein TOPAZ1 Source.170: DFBPPR13977 ---- Animal proteins ---- 40S ribosomal protein S25 Source.171: DFBPPR13990 ---- Animal proteins ---- Myosin heavy chain, fast skeletal muscle Source.172: DFBPPR14229 ---- Marine protein ---- UPF0739 protein C1orf74 homolog Source.173: DFBPPR14348 ---- Marine protein ---- Tubulin beta chain Source.174: DFBPPR14368 ---- Marine protein ---- Probable transcriptional regulator ycf27 Source.175: DFBPPR14370 ---- Marine protein ---- DNA-directed RNA polymerase subunit beta'' Source.176: DFBPPR14380 ---- Marine protein ---- tRNA(Ile)-lysidine synthase, chloroplastic Source.177: DFBPPR14806 ---- Marine protein ---- Tubulin beta-2 chain Source.178: DFBPPR14807 ---- Marine protein ---- Tubulin beta-1 chain Source.179: DFBPPR14964 ---- Microorganism protein ---- Arginine biosynthesis bifunctional protein ArgJ, mitochondrial Source.180: DFBPPR15005 ---- Microorganism protein ---- Origin recognition complex subunit 1 Source.181: DFBPPR15065 ---- Microorganism protein ---- Lactose regulatory protein LAC9 Source.182: DFBPPR15179 ---- Microorganism protein ---- ATP-dependent RNA helicase MRH4, mitochondrial Source.183: DFBPPR15249 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 9 Source.184: DFBPPR15354 ---- Microorganism protein ---- Sterol 24-C-methyltransferase Source.185: DFBPPR15392 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 16 Source.186: DFBPPR15466 ---- Microorganism protein ---- Cytosolic Fe-S cluster assembly factor NAR1 Source.187: DFBPPR15480 ---- Microorganism protein ---- Outer spore wall assembly protein SHE10 Source.188: DFBPPR15553 ---- Microorganism protein ---- Structure-specific endonuclease subunit SLX4 Source.189: DFBPPR15744 ---- Microorganism protein ---- Peroxisomal membrane protein PEX21 Source.190: DFBPPR15861 ---- Microorganism protein ---- Pyruvate kinase Source.191: DFBPPR7777 ---- Plant protein ---- Arginine biosynthesis bifunctional protein ArgJ, chloroplastic Source.192: DFBPPR7895 ---- Plant protein ---- CASP-like protein UU-1 Source.193: DFBPPR8041 ---- Plant protein ---- Nitrate reductase [NADH] 2 Source.194: DFBPPR8072 ---- Plant protein ---- Phosphoenolpyruvate carboxylase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide was chemically synthesised and evaluated for hydroxyl radical scavenging activity, it was a potential antioxidant peptide (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	M. veneriformis flesh hydrolysate was produced by incubating under optimal conditions with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Liu R, Zheng W, Li J, Wang L, Wu H, Wang X, Shi L. Rapid identification of bioactive peptides with antioxidant activity from the enzymatic hydrolysate of Mactra veneriformis by UHPLC-Q-TOF mass spectrometry. Food Chem. 2015 Jan 15;167:484-9. PMID: 25149015 DOI: 10.1016/j.foodchem.2014.06.113
Other literature(s)	N.D
PubDate	2015