Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0472(Antioxidative peptide)

DFBP ID	DFBPANOX0472
Peptide sequence	VCSV
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Cys-Ser-Val

Single-letter amino acid

VCSV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	406.50 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.33 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

2.5250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Fish, Marine
Organism/Source	Flounder fish (Paralichthys olivaceus)
Precursor protein	Flounder fish muscle protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0857 ---- Plant proteins ---- Mitogen-activated protein kinase 5 Source.2: DFBPPR0913 ---- Plant proteins ---- Endoribonuclease Dicer homolog 4 Source.3: DFBPPR2005 ---- Plant proteins ---- Telomerase reverse transcriptase Source.4: DFBPPR2093 ---- Plant proteins ---- Ent-kaurene oxidase-like 5 Source.5: DFBPPR2259 ---- Plant proteins ---- Inorganic phosphate transporter 1-1 Source.6: DFBPPR2589 ---- Plant proteins ---- Probable solanesyl-diphosphate synthase 3, chloroplastic Source.7: DFBPPR2674 ---- Plant proteins ---- Probable inorganic phosphate transporter 1-3 Source.8: DFBPPR2746 ---- Plant proteins ---- Potassium transporter 10 Source.9: DFBPPR3555 ---- Plant proteins ---- Actin-related protein 8 Source.10: DFBPPR4672 ---- Plant proteins ---- Ninja-family protein Os03g0419100 Source.11: DFBPPR4712 ---- Plant proteins ---- Putative UPF0496 protein 5 Source.12: DFBPPR4868 ---- Plant proteins ---- Putative B3 domain-containing protein Os08g0325100 Source.13: DFBPPR5663 ---- Plant proteins ---- Arginine biosynthesis bifunctional protein ArgJ, chloroplastic Source.14: DFBPPR6145 ---- Plant proteins ---- Ninja-family protein 6 Source.15: DFBPPR6923 ---- Plant proteins ---- Avenin-like a1 Source.16: DFBPPR6961 ---- Plant proteins ---- Avenin-like a2 Source.17: DFBPPR6964 ---- Plant proteins ---- Avenin-like a3 Source.18: DFBPPR6971 ---- Plant proteins ---- Avenin-like a7 Source.19: DFBPPR6973 ---- Plant proteins ---- Avenin-like a6 Source.20: DFBPPR6983 ---- Plant proteins ---- Avenin-like a4 Source.21: DFBPPR16103 ---- Animal proteins ---- Laforin Source.22: DFBPPR16202 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 3 Source.23: DFBPPR16609 ---- Animal proteins ---- DLA class II histocompatibility antigen, DR-1 beta chain Source.24: DFBPPR17232 ---- Animal proteins ---- Angiopoietin-1 receptor Source.25: DFBPPR17233 ---- Animal proteins ---- Angiopoietin-1 receptor Source.26: DFBPPR17344 ---- Animal proteins ---- Neurexin-1 Source.27: DFBPPR17502 ---- Animal proteins ---- V-type proton ATPase subunit B, kidney isoform Source.28: DFBPPR17938 ---- Animal proteins ---- Probable phospholipid-transporting ATPase IA Source.29: DFBPPR18105 ---- Animal proteins ---- Ras GTPase-activating protein 1 Source.30: DFBPPR18140 ---- Animal proteins ---- Semaphorin-3C Source.31: DFBPPR18565 ---- Animal proteins ---- Glycerol-3-phosphate acyltransferase 4 Source.32: DFBPPR18907 ---- Animal proteins ---- Recombining binding protein suppressor of hairless Source.33: DFBPPR18945 ---- Animal proteins ---- Stanniocalcin-1 Source.34: DFBPPR18951 ---- Animal proteins ---- DNA excision repair protein ERCC-8 Source.35: DFBPPR19441 ---- Animal proteins ---- Keratin, type II cytoskeletal 71 Source.36: DFBPPR19548 ---- Animal proteins ---- Reticulophagy regulator 1 Source.37: DFBPPR19879 ---- Animal proteins ---- TBC1 domain family member 14 Source.38: DFBPPR20302 ---- Animal proteins ---- General transcription factor IIH subunit 3 Source.39: DFBPPR20664 ---- Animal proteins ---- General transcription factor IIH subunit 2 Source.40: DFBPPR20720 ---- Animal proteins ---- BoLa class II histocompatibility antigen, DQB*0101 beta chain Source.41: DFBPPR21294 ---- Animal proteins ---- Actin filament-associated protein 1-like 1 Source.42: DFBPPR21298 ---- Animal proteins ---- SH3KBP1-binding protein 1 Source.43: DFBPPR21695 ---- Animal proteins ---- Choline transporter-like protein 3 Source.44: DFBPPR21802 ---- Animal proteins ---- Antigen WC1.1 Source.45: DFBPPR22043 ---- Animal proteins ---- Leukocyte antigen CD37 Source.46: DFBPPR8557 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 1 Source.47: DFBPPR9861 ---- Animal proteins ---- Major facilitator superfamily domain-containing protein 6 Source.48: DFBPPR9939 ---- Animal proteins ---- Tctex1 domain-containing protein 4 Source.49: DFBPPR10180 ---- Animal proteins ---- Teneurin-2 Source.50: DFBPPR10442 ---- Animal proteins ---- Neurexin-1 Source.51: DFBPPR10735 ---- Animal proteins ---- Semaphorin-3E Source.52: DFBPPR10963 ---- Animal proteins ---- Semaphorin-3C Source.53: DFBPPR12076 ---- Animal proteins ---- RRP12-like protein Source.54: DFBPPR12118 ---- Animal proteins ---- Protein EURL Source.55: DFBPPR12184 ---- Animal proteins ---- GRIN2-like protein Source.56: DFBPPR12323 ---- Animal proteins ---- Flavin-containing monooxygenase 5 Source.57: DFBPPR12464 ---- Animal proteins ---- Urokinase-type plasminogen activator Source.58: DFBPPR12625 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 4 Source.59: DFBPPR14698 ---- Marine protein ---- Cystatin Source.60: DFBPPR14889 ---- Microorganism protein ---- Glucokinase-1 Source.61: DFBPPR14902 ---- Microorganism protein ---- Lysophospholipase Source.62: DFBPPR7777 ---- Plant protein ---- Arginine biosynthesis bifunctional protein ArgJ, chloroplastic Source.63: DFBPPR8040 ---- Plant protein ---- Nitrate reductase [NADH] 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity

The peptide VCSV showed good scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical (IC₅₀ value, 111.32 μM) and high cytoprotective activities against 2,2-azobis-(2-amidino-propane) dihydrochloride (AAPH) without cytotoxicity and scavenged total reactive oxygen species in Vero cells.

Table 1. The antioxidant activity of purified peptide VCSV.
Purified peptide	Radicals (IC₅₀ mg/ml)
Purified peptide	DPPH	Hydroxyl	Peroxyl
VCSV	0.045	0.253	0.006

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The α-chymotrypsin hydrolysate showed the strongest antioxidant activity among the eight enzymatic hydrolysates (eight commercial proteases such as papain, pepsin, trypsin, neutrase, alcalase, kojizyme, protamex, and α-chymotrypsin).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The antioxidative peptide VCSV could be useful as therapeutic agents.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Ko JY, Lee JH, Samarakoon K, Kim JS, Jeon YJ. Purification and determination of two novel antioxidant peptides from flounder fish (Paralichthys olivaceus) using digestive proteases. Food Chem Toxicol. 2013 Feb;52:113-20. PMID: 23146692 DOI: 10.1016/j.fct.2012.10.058
Other literature(s)	N.D
PubDate	2013