Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0498(Antioxidative peptide)

DFBP ID	DFBPANOX0498
Peptide sequence	ELLI
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Leu-Leu-Ile

Single-letter amino acid

ELLI

Peptide length

Peptide mass

Experimental mass	Theoretical mass
486.7 Da	486.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

2.1500^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Spotless smoothhound (Mustelus griseus)
Precursor protein	Ethanol-soluble protein, muscle protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0937 ---- Plant proteins ---- Glutamate synthase 1 [NADH], chloroplastic Source.2: DFBPPR0939 ---- Plant proteins ---- Protein argonaute MEL1 Source.3: DFBPPR0954 ---- Plant proteins ---- Leucine-rich repeat receptor protein kinase MSP1 Source.4: DFBPPR1069 ---- Plant proteins ---- Cinnamoyl-CoA reductase 1 Source.5: DFBPPR1303 ---- Plant proteins ---- Inositol-tetrakisphosphate 1-kinase 6 Source.6: DFBPPR1328 ---- Plant proteins ---- Probable ethylene response sensor 1 Source.7: DFBPPR1412 ---- Plant proteins ---- Histone H3.3 Source.8: DFBPPR1555 ---- Plant proteins ---- Cytochrome P450 734A6 Source.9: DFBPPR1567 ---- Plant proteins ---- Protein argonaute PNH1 Source.10: DFBPPR1811 ---- Plant proteins ---- Sulfhydryl oxidase 1 Source.11: DFBPPR1830 ---- Plant proteins ---- Endoribonuclease Dicer homolog 3a Source.12: DFBPPR1906 ---- Plant proteins ---- Ribose-phosphate pyrophosphokinase 1, chloroplastic Source.13: DFBPPR1940 ---- Plant proteins ---- Histone H3.2 Source.14: DFBPPR1955 ---- Plant proteins ---- Endoribonuclease Dicer homolog 3b Source.15: DFBPPR2055 ---- Plant proteins ---- Cytokinin dehydrogenase 7 Source.16: DFBPPR2086 ---- Plant proteins ---- Cytokinin dehydrogenase 6 Source.17: DFBPPR2087 ---- Plant proteins ---- Cytokinin dehydrogenase 10 Source.18: DFBPPR2168 ---- Plant proteins ---- DnaJ protein ERDJ2 Source.19: DFBPPR2236 ---- Plant proteins ---- Auxin response factor 24 Source.20: DFBPPR2327 ---- Plant proteins ---- Kinesin-like protein KIN-7K, chloroplastic Source.21: DFBPPR2829 ---- Plant proteins ---- CDP-diacylglycerol--serine O-phosphatidyltransferase 2 Source.22: DFBPPR3237 ---- Plant proteins ---- Arginine decarboxylase 2 Source.23: DFBPPR3288 ---- Plant proteins ---- Chitin-inducible gibberellin-responsive protein 2 Source.24: DFBPPR3417 ---- Plant proteins ---- Protein CutA 1, chloroplastic Source.25: DFBPPR3613 ---- Plant proteins ---- Transcription factor PCF6 Source.26: DFBPPR3623 ---- Plant proteins ---- Kinesin-like protein KIN-14K Source.27: DFBPPR3734 ---- Plant proteins ---- Cation transporter HKT8 Source.28: DFBPPR4053 ---- Plant proteins ---- Protein argonaute 1B Source.29: DFBPPR4121 ---- Plant proteins ---- Protein argonaute 1C Source.30: DFBPPR4283 ---- Plant proteins ---- SCAR-like protein 1 Source.31: DFBPPR4347 ---- Plant proteins ---- Metal transporter Nramp2 Source.32: DFBPPR4401 ---- Plant proteins ---- Phospholipase A1-II 2 Source.33: DFBPPR4453 ---- Plant proteins ---- Protein EXECUTER 1, chloroplastic Source.34: DFBPPR4454 ---- Plant proteins ---- Chloroplast envelope membrane protein Source.35: DFBPPR4540 ---- Plant proteins ---- Protein argonaute 1A Source.36: DFBPPR4549 ---- Plant proteins ---- Ammonium transporter 3 member 3 Source.37: DFBPPR4578 ---- Plant proteins ---- Protein argonaute 1D Source.38: DFBPPR4711 ---- Plant proteins ---- Putative zinc finger CCCH domain-containing protein 51 Source.39: DFBPPR4818 ---- Plant proteins ---- Probable protein transport Sec1b Source.40: DFBPPR4826 ---- Plant proteins ---- Probable protein transport Sec1a Source.41: DFBPPR4937 ---- Plant proteins ---- Glutamate synthase 2 [NADH], chloroplastic Source.42: DFBPPR4939 ---- Plant proteins ---- Telomere-binding protein 1 Source.43: DFBPPR5021 ---- Plant proteins ---- Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic Source.44: DFBPPR5294 ---- Plant proteins ---- Protein Ycf2 Source.45: DFBPPR5489 ---- Plant proteins ---- Histone H3.2 Source.46: DFBPPR5685 ---- Plant proteins ---- Protein ABERRANT POLLEN TRANSMISSION 1 Source.47: DFBPPR6030 ---- Plant proteins ---- DNA polymerase Source.48: DFBPPR6065 ---- Plant proteins ---- Chloroplast envelope membrane protein Source.49: DFBPPR6247 ---- Plant proteins ---- DNA topoisomerase 2 Source.50: DFBPPR6258 ---- Plant proteins ---- Histone H3.2 Source.51: DFBPPR6342 ---- Plant proteins ---- Ent-copalyl diphosphate synthase, chloroplastic Source.52: DFBPPR6456 ---- Plant proteins ---- Nucleoside-triphosphatase Source.53: DFBPPR6686 ---- Plant proteins ---- Histone H3.2 Source.54: DFBPPR7039 ---- Plant proteins ---- Nitrate reductase [NAD(P)H] Source.55: DFBPPR7229 ---- Plant proteins ---- Histone H3.3 Source.56: DFBPPR7410 ---- Plant proteins ---- Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic Source.57: DFBPPR7448 ---- Plant proteins ---- Histone H3.2 Source.58: DFBPPR7470 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.59: DFBPPR7738 ---- Plant proteins ---- Arginine decarboxylase Source.60: DFBPPR8375 ---- Plant proteins ---- Psoralen synthase Source.61: DFBPPR16039 ---- Animal proteins ---- Breast cancer type 1 susceptibility protein homolog Source.62: DFBPPR16105 ---- Animal proteins ---- Collagen alpha-1(XVII) chain Source.63: DFBPPR16243 ---- Animal proteins ---- Retinoic acid receptor RXR-beta Source.64: DFBPPR16475 ---- Animal proteins ---- Transmembrane protein 258 Source.65: DFBPPR16752 ---- Animal proteins ---- Zinc finger BED domain-containing protein 5 Source.66: DFBPPR16778 ---- Animal proteins ---- Prolactin receptor Source.67: DFBPPR16847 ---- Animal proteins ---- Fibrinogen alpha chain Source.68: DFBPPR16939 ---- Animal proteins ---- Histone H3.3 Source.69: DFBPPR17051 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.70: DFBPPR17064 ---- Animal proteins ---- Unconventional myosin-Ic Source.71: DFBPPR17279 ---- Animal proteins ---- Stimulator of interferon genes protein Source.72: DFBPPR17372 ---- Animal proteins ---- ATP-dependent RNA helicase DDX1 Source.73: DFBPPR17533 ---- Animal proteins ---- Carboxypeptidase E Source.74: DFBPPR17581 ---- Animal proteins ---- Histone H3.1 Source.75: DFBPPR17613 ---- Animal proteins ---- Collagen alpha-1(XVII) chain Source.76: DFBPPR17744 ---- Animal proteins ---- Protein argonaute-2 Source.77: DFBPPR17779 ---- Animal proteins ---- Integrin alpha-3 Source.78: DFBPPR17912 ---- Animal proteins ---- Histone H3.2 Source.79: DFBPPR17964 ---- Animal proteins ---- Ribose-phosphate pyrophosphokinase 1 Source.80: DFBPPR18224 ---- Animal proteins ---- Integral membrane protein 2B Source.81: DFBPPR18239 ---- Animal proteins ---- Nucleobindin-1 Source.82: DFBPPR18316 ---- Animal proteins ---- DNA-directed primase/polymerase protein Source.83: DFBPPR18330 ---- Animal proteins ---- Retinoic acid receptor RXR-gamma Source.84: DFBPPR18567 ---- Animal proteins ---- Dynein heavy chain 12, axonemal Source.85: DFBPPR18577 ---- Animal proteins ---- Neutral amino acid transporter B(0) Source.86: DFBPPR18654 ---- Animal proteins ---- SMC5-SMC6 complex localization factor protein 1 Source.87: DFBPPR18741 ---- Animal proteins ---- Coatomer subunit beta' Source.88: DFBPPR18769 ---- Animal proteins ---- Histone H3.3C Source.89: DFBPPR18817 ---- Animal proteins ---- Protein argonaute-3 Source.90: DFBPPR18877 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.91: DFBPPR18897 ---- Animal proteins ---- Kelch-like protein 20 Source.92: DFBPPR18993 ---- Animal proteins ---- Dynein regulatory complex protein 1 Source.93: DFBPPR19162 ---- Animal proteins ---- Macrophage-capping protein Source.94: DFBPPR19452 ---- Animal proteins ---- Mitochondrial amidoxime reducing component 2 Source.95: DFBPPR19528 ---- Animal proteins ---- Methionine--tRNA ligase, cytoplasmic Source.96: DFBPPR19571 ---- Animal proteins ---- Tonsoku-like protein Source.97: DFBPPR19761 ---- Animal proteins ---- N-chimaerin Source.98: DFBPPR19833 ---- Animal proteins ---- Contactin-1 Source.99: DFBPPR20071 ---- Animal proteins ---- Glutathione S-transferase A4 Source.100: DFBPPR20196 ---- Animal proteins ---- Mediator of RNA polymerase II transcription subunit 4 Source.101: DFBPPR20599 ---- Animal proteins ---- Cleavage and polyadenylation specificity factor subunit 1 Source.102: DFBPPR20790 ---- Animal proteins ---- DNA-directed RNA polymerases I, II, and III subunit RPABC1 Source.103: DFBPPR20921 ---- Animal proteins ---- TRAF-type zinc finger domain-containing protein 1 Source.104: DFBPPR21014 ---- Animal proteins ---- Transmembrane protein 258 Source.105: DFBPPR21169 ---- Animal proteins ---- GA-binding protein subunit beta-2 Source.106: DFBPPR21217 ---- Animal proteins ---- GA-binding protein subunit beta-1 Source.107: DFBPPR21324 ---- Animal proteins ---- Transmembrane protein 115 Source.108: DFBPPR21379 ---- Animal proteins ---- Phosphoribosyl pyrophosphate synthase-associated protein 2 Source.109: DFBPPR21404 ---- Animal proteins ---- Phosphoribosyl pyrophosphate synthase-associated protein 1 Source.110: DFBPPR21407 ---- Animal proteins ---- Ribosome biogenesis protein BRX1 homolog Source.111: DFBPPR21434 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 15 Source.112: DFBPPR21730 ---- Animal proteins ---- Post-GPI attachment to proteins factor 2 Source.113: DFBPPR21904 ---- Animal proteins ---- Protein TBATA Source.114: DFBPPR21962 ---- Animal proteins ---- Tetraspanin-3 Source.115: DFBPPR22165 ---- Animal proteins ---- Coiled-coil domain-containing protein 106 Source.116: DFBPPR22448 ---- Animal proteins ---- Transmembrane and coiled-coil domain-containing protein 5B Source.117: DFBPPR22488 ---- Animal proteins ---- Zinc finger BED domain-containing protein 5 Source.118: DFBPPR22549 ---- Animal proteins ---- Isochorismatase domain-containing protein 1 Source.119: DFBPPR22710 ---- Animal proteins ---- RIIa domain-containing protein 1 Source.120: DFBPPR8606 ---- Animal proteins ---- Stimulator of interferon genes protein Source.121: DFBPPR8666 ---- Animal proteins ---- Histone H3.3 Source.122: DFBPPR8825 ---- Animal proteins ---- Optineurin Source.123: DFBPPR8887 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.124: DFBPPR9049 ---- Animal proteins ---- Integral membrane protein 2B Source.125: DFBPPR9075 ---- Animal proteins ---- Retinoic acid receptor RXR-gamma Source.126: DFBPPR9322 ---- Animal proteins ---- Solute carrier family 5 member 4 Source.127: DFBPPR9940 ---- Animal proteins ---- Neuronatin Source.128: DFBPPR9960 ---- Animal proteins ---- Histone H3.2 Source.129: DFBPPR10043 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.130: DFBPPR10070 ---- Animal proteins ---- Vesicle-associated membrane protein 7 Source.131: DFBPPR10130 ---- Animal proteins ---- Histone H3.3 Source.132: DFBPPR10334 ---- Animal proteins ---- Retinoic acid receptor RXR-gamma Source.133: DFBPPR10385 ---- Animal proteins ---- Centromere protein S Source.134: DFBPPR10411 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.135: DFBPPR10482 ---- Animal proteins ---- ATP-dependent RNA helicase DDX1 Source.136: DFBPPR10499 ---- Animal proteins ---- Prolactin receptor Source.137: DFBPPR10506 ---- Animal proteins ---- Ribose-phosphate pyrophosphokinase 2 Source.138: DFBPPR10606 ---- Animal proteins ---- Unconventional myosin-Ic Source.139: DFBPPR10653 ---- Animal proteins ---- Contactin-1 Source.140: DFBPPR10984 ---- Animal proteins ---- Kelch-like protein 20 Source.141: DFBPPR11191 ---- Animal proteins ---- Protein argonaute-3 Source.142: DFBPPR11224 ---- Animal proteins ---- Nuclear receptor subfamily 5 group A member 2 Source.143: DFBPPR11265 ---- Animal proteins ---- Integral membrane protein 2B Source.144: DFBPPR11613 ---- Animal proteins ---- Transmembrane protein 258 Source.145: DFBPPR11816 ---- Animal proteins ---- Sister chromatid cohesion protein PDS5 homolog A Source.146: DFBPPR11872 ---- Animal proteins ---- Protein argonaute-4 Source.147: DFBPPR12005 ---- Animal proteins ---- Phosphoribosyl pyrophosphate synthase-associated protein 2 Source.148: DFBPPR12147 ---- Animal proteins ---- Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C Source.149: DFBPPR12199 ---- Animal proteins ---- Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B Source.150: DFBPPR12212 ---- Animal proteins ---- GTPase-activating Rap/Ran-GAP domain-like protein 3 Source.151: DFBPPR12272 ---- Animal proteins ---- Large neutral amino acids transporter small subunit 1 Source.152: DFBPPR12275 ---- Animal proteins ---- Ryanodine receptor 3 Source.153: DFBPPR12290 ---- Animal proteins ---- Ryanodine receptor 2 Source.154: DFBPPR12343 ---- Animal proteins ---- Protein SLFN14 Source.155: DFBPPR12440 ---- Animal proteins ---- Protein argonaute-2 Source.156: DFBPPR12816 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.157: DFBPPR13005 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 4 Source.158: DFBPPR13010 ---- Animal proteins ---- Sodium- and chloride-dependent betaine transporter Source.159: DFBPPR13048 ---- Animal proteins ---- Myosin regulatory light chain 2, skeletal muscle isoform type 1 Source.160: DFBPPR13947 ---- Animal proteins ---- Cortactin-binding protein 2 Source.161: DFBPPR14297 ---- Marine protein ---- Probable transcriptional regulator ycf27 Source.162: DFBPPR14333 ---- Marine protein ---- Ferredoxin-dependent glutamate synthase Source.163: DFBPPR14560 ---- Marine protein ---- Histone H3.2 Source.164: DFBPPR14944 ---- Microorganism protein ---- Histone H3 Source.165: DFBPPR14961 ---- Microorganism protein ---- Alcohol dehydrogenase 1 Source.166: DFBPPR14982 ---- Microorganism protein ---- Cytochrome P450 monooxygenase PUL2 Source.167: DFBPPR15113 ---- Microorganism protein ---- NADH-cytochrome b5 reductase 1 Source.168: DFBPPR15180 ---- Microorganism protein ---- Protein ROT1 Source.169: DFBPPR15186 ---- Microorganism protein ---- Alcohol dehydrogenase 2 Source.170: DFBPPR15212 ---- Microorganism protein ---- Protein transport protein SEC23 Source.171: DFBPPR15230 ---- Microorganism protein ---- Histone acetyltransferase type B subunit 2 Source.172: DFBPPR15319 ---- Microorganism protein ---- Glucose transport transcription regulator RGT1 Source.173: DFBPPR15421 ---- Microorganism protein ---- Cytochrome c lysine N-methyltransferase 1 Source.174: DFBPPR15455 ---- Microorganism protein ---- Putative tyrosine-protein phosphatase OCA1 Source.175: DFBPPR15536 ---- Microorganism protein ---- Protein SDS23 Source.176: DFBPPR15580 ---- Microorganism protein ---- Oligosaccharide translocation protein RFT1 Source.177: DFBPPR15599 ---- Microorganism protein ---- Pre-mRNA-splicing factor SYF1 Source.178: DFBPPR7912 ---- Plant protein ---- Chloroplast envelope membrane protein Source.179: DFBPPR8155 ---- Plant protein ---- Protein Ycf2 Source.180: DFBPPR8300 ---- Plant protein ---- Protein Ycf2 Source.181: DFBPPR8354 ---- Plant protein ---- Pollen-specific protein SF21
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	(1) The purified peptide ELLI exhibited high scavenging activity on hydroxyl radical (IC₅₀ 0.1173 mg/ml), ABTS radical (IC₅₀ 0.3274 mg/ml), and superoxide radical (IC₅₀ 0.1851 mg/ml). (2) The purified peptide also effectively inhibited the autooxidation in linoleic acid model system.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The ethanol-soluble proteins antioxidant hydrolysates were prepared using papain.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptides isolated from the ethanol-soluble proteins hydrolysates of spotless smoothhound muscle were potent antioxidants and might be effectively used as food additives and pharmaceutical agents.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wang, B., Gong, Y.-D., Li, Z.-R., Yu, D., Chi, C.-F., Ma, J.-Y. Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle. Journal of Functional Foods. 2014, 6, 176-85. DOI: 10.1016/j.jff.2013.10.004
Other literature(s)	[1] Zou, T.B., et al., The Structure-Activity Relationship of the Antioxidant Peptides from Natural Proteins. Molecules, 2016. 21(1): p. 72.
PubDate	2014