Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0499(Antioxidative peptide)

DFBP ID	DFBPANOX0499
Peptide sequence	KFPE
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Phe-Pro-Glu

Single-letter amino acid

KFPE

Peptide length

Peptide mass

Experimental mass	Theoretical mass
519.6 Da	519.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Spotless smoothhound (Mustelus griseus)
Precursor protein	Ethanol-soluble protein, muscle protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0927 ---- Plant proteins ---- Serine/threonine-protein kinase TOR Source.2: DFBPPR1546 ---- Plant proteins ---- Potassium transporter 1 Source.3: DFBPPR1632 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 6, chloroplastic Source.4: DFBPPR2135 ---- Plant proteins ---- Putative DNA ligase 4 Source.5: DFBPPR2406 ---- Plant proteins ---- Probable serine/threonine-protein kinase WNK6 Source.6: DFBPPR2464 ---- Plant proteins ---- Two-component response regulator ORR25 Source.7: DFBPPR2881 ---- Plant proteins ---- Probable protein NAP1 Source.8: DFBPPR3602 ---- Plant proteins ---- Coatomer subunit alpha-2 Source.9: DFBPPR3822 ---- Plant proteins ---- Coatomer subunit alpha-3 Source.10: DFBPPR3926 ---- Plant proteins ---- Probable potassium transporter 13 Source.11: DFBPPR3928 ---- Plant proteins ---- Coatomer subunit alpha-1 Source.12: DFBPPR4026 ---- Plant proteins ---- Potassium transporter 19 Source.13: DFBPPR4027 ---- Plant proteins ---- Potassium transporter 20 Source.14: DFBPPR4096 ---- Plant proteins ---- Cytochrome c biogenesis protein CCS1, chloroplastic Source.15: DFBPPR6475 ---- Plant proteins ---- Probable aquaporin PIP-type 7a Source.16: DFBPPR16205 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Source.17: DFBPPR16260 ---- Animal proteins ---- Creatine kinase B-type Source.18: DFBPPR16327 ---- Animal proteins ---- Pituitary-specific positive transcription factor 1 Source.19: DFBPPR16590 ---- Animal proteins ---- Arylsulfatase K Source.20: DFBPPR16647 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.21: DFBPPR16720 ---- Animal proteins ---- Tudor domain-containing protein 5 Source.22: DFBPPR16986 ---- Animal proteins ---- Aspartyl/asparaginyl beta-hydroxylase Source.23: DFBPPR17262 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 33 Source.24: DFBPPR17354 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.25: DFBPPR17450 ---- Animal proteins ---- Adhesion G protein-coupled receptor L3 Source.26: DFBPPR17527 ---- Animal proteins ---- Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Source.27: DFBPPR17609 ---- Animal proteins ---- Fructose-1,6-bisphosphatase isozyme 2 Source.28: DFBPPR17678 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 16 Source.29: DFBPPR17899 ---- Animal proteins ---- Eukaryotic translation initiation factor 2 subunit 1 Source.30: DFBPPR18540 ---- Animal proteins ---- Fibroblast growth factor-binding protein 1 Source.31: DFBPPR18647 ---- Animal proteins ---- Creatine kinase B-type Source.32: DFBPPR18757 ---- Animal proteins ---- Mevalonate kinase Source.33: DFBPPR19409 ---- Animal proteins ---- Hyaluronan-binding protein 2 Source.34: DFBPPR20405 ---- Animal proteins ---- Oxygen-regulated protein 1 Source.35: DFBPPR20550 ---- Animal proteins ---- G-protein coupled receptor family C group 6 member A Source.36: DFBPPR20641 ---- Animal proteins ---- Centrosomal protein of 41 kDa Source.37: DFBPPR20850 ---- Animal proteins ---- Arylsulfatase K Source.38: DFBPPR21118 ---- Animal proteins ---- NADH-cytochrome b5 reductase 1 Source.39: DFBPPR21666 ---- Animal proteins ---- Importin-13 Source.40: DFBPPR22455 ---- Animal proteins ---- Phosducin-like protein 2 Source.41: DFBPPR22512 ---- Animal proteins ---- IQ domain-containing protein C Source.42: DFBPPR8682 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.43: DFBPPR9123 ---- Animal proteins ---- Eukaryotic translation initiation factor 2 subunit 1 Source.44: DFBPPR9406 ---- Animal proteins ---- Creatine kinase B-type Source.45: DFBPPR9407 ---- Animal proteins ---- Creatine kinase B-type Source.46: DFBPPR9749 ---- Animal proteins ---- Guanylin Source.47: DFBPPR9977 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.48: DFBPPR10282 ---- Animal proteins ---- Reversion-inducing cysteine-rich protein with Kazal motifs Source.49: DFBPPR10361 ---- Animal proteins ---- Protein HIRA Source.50: DFBPPR10451 ---- Animal proteins ---- Eukaryotic translation initiation factor 2 subunit 1 Source.51: DFBPPR10457 ---- Animal proteins ---- Transcriptional enhancer factor TEF-3 Source.52: DFBPPR11037 ---- Animal proteins ---- Disheveled-associated activator of morphogenesis 2 Source.53: DFBPPR11184 ---- Animal proteins ---- Small RNA 2'-O-methyltransferase Source.54: DFBPPR11251 ---- Animal proteins ---- Transcriptional enhancer factor TEF-5 Source.55: DFBPPR11352 ---- Animal proteins ---- Pituitary-specific positive transcription factor 1 Source.56: DFBPPR11413 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.57: DFBPPR11595 ---- Animal proteins ---- Histone-lysine N-methyltransferase SETD1B Source.58: DFBPPR11602 ---- Animal proteins ---- Arylsulfatase K Source.59: DFBPPR11698 ---- Animal proteins ---- NADH-cytochrome b5 reductase 2 Source.60: DFBPPR11988 ---- Animal proteins ---- Transmembrane channel-like protein 3 Source.61: DFBPPR11999 ---- Animal proteins ---- Dymeclin Source.62: DFBPPR12055 ---- Animal proteins ---- Importin-13 Source.63: DFBPPR12135 ---- Animal proteins ---- Vigilin Source.64: DFBPPR12438 ---- Animal proteins ---- Fructose-1,6-bisphosphatase isozyme 2 Source.65: DFBPPR14258 ---- Marine protein ---- Pituitary-specific positive transcription factor 1 Source.66: DFBPPR14658 ---- Marine protein ---- Pituitary-specific positive transcription factor 1 Source.67: DFBPPR14973 ---- Microorganism protein ---- Lon protease homolog, mitochondrial Source.68: DFBPPR14998 ---- Microorganism protein ---- Galactokinase Source.69: DFBPPR15150 ---- Microorganism protein ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.70: DFBPPR15190 ---- Microorganism protein ---- Pescadillo homolog Source.71: DFBPPR15366 ---- Microorganism protein ---- DNA-directed RNA polymerases I, II, and III subunit RPABC1 Source.72: DFBPPR15392 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 16 Source.73: DFBPPR15502 ---- Microorganism protein ---- Mitochondrial distribution and morphology protein 32 Source.74: DFBPPR15516 ---- Microorganism protein ---- mRNA 3'-end-processing protein RNA14 Source.75: DFBPPR15645 ---- Microorganism protein ---- Putative transferase CAF17, mitochondrial Source.76: DFBPPR15646 ---- Microorganism protein ---- Altered inheritance of mitochondria protein 36, mitochondrial Source.77: DFBPPR7946 ---- Plant protein ---- Aquaporin PIP1.1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	(1) The purified peptide KFPE exhibited high scavenging activity on hydroxyl radical (IC₅₀ 0.1510 mg/ml), ABTS radical (IC₅₀ 0.4645 mg/ml), and superoxide radical (IC₅₀ 0.2053 mg/ml). (2) The purified peptide also effectively inhibited the autooxidation in linoleic acid model system.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The ethanol-soluble proteins antioxidant hydrolysates were prepared using papain.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptides isolated from the ethanol-soluble proteins hydrolysates of spotless smoothhound muscle were potent antioxidants and might be effectively used as food additives and pharmaceutical agents.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wang, B., Gong, Y.-D., Li, Z.-R., Yu, D., Chi, C.-F., Ma, J.-Y. Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle. Journal of Functional Foods. 2014, 6, 176-85. DOI: 10.1016/j.jff.2013.10.004
Other literature(s)	[1] Zou, T.B., et al., The Structure-Activity Relationship of the Antioxidant Peptides from Natural Proteins. Molecules, 2016. 21(1): p. 72.
PubDate	2014