Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0503(Antioxidative peptide)

DFBP ID	DFBPANOX0503
Peptide sequence	PSLPA
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Pro-Ser-Leu-Pro-Ala

Single-letter amino acid

PSLPA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
484.3 Da	483.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.3200^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hemp (Cannabis sativa L.)
Precursor protein	Hemp seed protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1006 ---- Plant proteins ---- Cellulose synthase A catalytic subunit 4 [UDP-forming] Source.2: DFBPPR1333 ---- Plant proteins ---- Endoglucanase 2 Source.3: DFBPPR1485 ---- Plant proteins ---- Pheophorbide a oxygenase, chloroplastic Source.4: DFBPPR1880 ---- Plant proteins ---- Putative laccase-11 Source.5: DFBPPR1994 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 Source.6: DFBPPR2037 ---- Plant proteins ---- Laccase-10 Source.7: DFBPPR2321 ---- Plant proteins ---- Aspartate carbamoyltransferase, chloroplastic Source.8: DFBPPR2535 ---- Plant proteins ---- Probable glucuronosyltransferase Os04g0650300 Source.9: DFBPPR3902 ---- Plant proteins ---- Probable serine acetyltransferase 5 Source.10: DFBPPR6163 ---- Plant proteins ---- Ninja-family protein 3 Source.11: DFBPPR7639 ---- Milk proteins ---- MICAL-like protein 2 Source.12: DFBPPR16265 ---- Animal proteins ---- Caspase-1 Source.13: DFBPPR17166 ---- Animal proteins ---- Cyclic nucleotide-gated cation channel beta-1 Source.14: DFBPPR17419 ---- Animal proteins ---- Inositol 1,4,5-triphosphate receptor associated 1 Source.15: DFBPPR17893 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 3 Source.16: DFBPPR18063 ---- Animal proteins ---- Microtubule-associated protein 1S Source.17: DFBPPR18134 ---- Animal proteins ---- Cyclin-T1 Source.18: DFBPPR18421 ---- Animal proteins ---- Matrix metalloproteinase-20 Source.19: DFBPPR18571 ---- Animal proteins ---- CREB-regulated transcription coactivator 2 Source.20: DFBPPR18901 ---- Animal proteins ---- Nuclear factor of activated T-cells, cytoplasmic 1 Source.21: DFBPPR18973 ---- Animal proteins ---- Transcriptional activator Myb Source.22: DFBPPR19254 ---- Animal proteins ---- Periaxin Source.23: DFBPPR19917 ---- Animal proteins ---- PH and SEC7 domain-containing protein 1 Source.24: DFBPPR20010 ---- Animal proteins ---- Craniofacial development protein 1 Source.25: DFBPPR20281 ---- Animal proteins ---- Splicing factor 3A subunit 2 Source.26: DFBPPR20370 ---- Animal proteins ---- 28S ribosomal protein S35, mitochondrial Source.27: DFBPPR20603 ---- Animal proteins ---- Craniofacial development protein 2 Source.28: DFBPPR20985 ---- Animal proteins ---- Transmembrane protein 131-like Source.29: DFBPPR21098 ---- Animal proteins ---- Pre T-cell antigen receptor alpha Source.30: DFBPPR22310 ---- Animal proteins ---- B-cell CLL/lymphoma 7 protein family member B Source.31: DFBPPR9102 ---- Animal proteins ---- Nuclear factor of activated T-cells, cytoplasmic 1 Source.32: DFBPPR9357 ---- Animal proteins ---- Interleukin-4 receptor subunit alpha Source.33: DFBPPR10351 ---- Animal proteins ---- Protein sidekick-2 Source.34: DFBPPR11101 ---- Animal proteins ---- Repulsive guidance molecule A Source.35: DFBPPR11691 ---- Animal proteins ---- Beta-crystallin A4 Source.36: DFBPPR11918 ---- Animal proteins ---- Nucleoside diphosphate-linked moiety X motif 19 Source.37: DFBPPR11949 ---- Animal proteins ---- Spindle assembly abnormal protein 6 homolog Source.38: DFBPPR12509 ---- Animal proteins ---- H(+)/Cl(-) exchange transporter 3
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	At 0.5 mg/mL, PSLPA was the most active antioxidant peptide among the hemp seed protein hydrolysates with 58% DPPH scavenging and metal chelation activity of 96%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hemp seed proteins was hydrolyzed by simulating gastrointestinal tract digestion (Pepsin and Pancreatin).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide had no inhibition for renin.

Database cross-references

DFBP

[D1]	DFBPACEI1279
[D2]	DFBPANHY0296
[D3]	DFBPMUFU0286

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Girgih, A.T., He, R., Malomo, S., Offengenden, M., Wu, J., Aluko, R.E. Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides. Journal of Functional Foods. 2014, 6, 384-94. DOI: 10.1016/j.jff.2013.11.005
Other literature(s)	[1] Zou, T.B., et al., The Structure-Activity Relationship of the Antioxidant Peptides from Natural Proteins. Molecules, 2016. 21(1): p. 72.
PubDate	2014