Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0511(Antioxidative peptide)

DFBP ID	DFBPANOX0511
Peptide sequence	FLPF
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Leu-Pro-Phe

Single-letter amino acid

FLPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
523.3 Da	522.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.9500^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Corn
Precursor protein	Corn protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0807 ---- Plant proteins ---- Protein EARLY HEADING DATE 2 Source.2: DFBPPR0928 ---- Plant proteins ---- Cytochrome P450 90B2 Source.3: DFBPPR0961 ---- Plant proteins ---- Ent-kaurenoic acid oxidase Source.4: DFBPPR0998 ---- Plant proteins ---- CBL-interacting protein kinase 31 Source.5: DFBPPR1326 ---- Plant proteins ---- Protein SHORTAGE IN CHIASMATA 1 homolog Source.6: DFBPPR1368 ---- Plant proteins ---- Cytochrome P450 90A4 Source.7: DFBPPR1493 ---- Plant proteins ---- Ent-isokaurene C2/C3-hydroxylase Source.8: DFBPPR1542 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-1 Source.9: DFBPPR1804 ---- Plant proteins ---- Ent-cassadiene hydroxylase Source.10: DFBPPR1989 ---- Plant proteins ---- CBL-interacting protein kinase 16 Source.11: DFBPPR2007 ---- Plant proteins ---- Plasma membrane ATPase Source.12: DFBPPR2544 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.13: DFBPPR2604 ---- Plant proteins ---- Auxin response factor 10 Source.14: DFBPPR2892 ---- Plant proteins ---- 30S ribosomal protein S18, chloroplastic Source.15: DFBPPR3184 ---- Plant proteins ---- Stromal processing peptidase, chloroplastic Source.16: DFBPPR3300 ---- Plant proteins ---- Calcineurin B-like protein 9 Source.17: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.18: DFBPPR3600 ---- Plant proteins ---- Transcription factor NIGTH1 Source.19: DFBPPR3648 ---- Plant proteins ---- Putative lipoxygenase 5 Source.20: DFBPPR3968 ---- Plant proteins ---- CRS2-associated factor 1, chloroplastic Source.21: DFBPPR3986 ---- Plant proteins ---- CRS2-associated factor 2, chloroplastic Source.22: DFBPPR4097 ---- Plant proteins ---- Trafficking protein particle complex II-specific subunit 130 homolog Source.23: DFBPPR4554 ---- Plant proteins ---- Zinc finger AN1 and C2H2 domain-containing stress-associated protein 16 Source.24: DFBPPR4604 ---- Plant proteins ---- Zinc finger AN1 domain-containing stress-associated protein 17 Source.25: DFBPPR4628 ---- Plant proteins ---- Probable inactive carboxylesterase Os04g0669700 Source.26: DFBPPR4655 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 7 Source.27: DFBPPR4931 ---- Plant proteins ---- Abscisic acid 8'-hydroxylase 2 Source.28: DFBPPR5186 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 6, chloroplastic Source.29: DFBPPR5225 ---- Plant proteins ---- Soyasapogenol B glucuronide galactosyltransferase Source.30: DFBPPR5277 ---- Plant proteins ---- Cytochrome P450 97B2, chloroplastic Source.31: DFBPPR5632 ---- Plant proteins ---- CRS2-associated factor 2, chloroplastic Source.32: DFBPPR5760 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.33: DFBPPR5859 ---- Plant proteins ---- 30S ribosomal protein S18, chloroplastic Source.34: DFBPPR6029 ---- Plant proteins ---- Zein-alpha PMS2 Source.35: DFBPPR6037 ---- Plant proteins ---- 19 kDa alpha-zein 19C2 Source.36: DFBPPR6057 ---- Plant proteins ---- Zein-alpha PMS1 Source.37: DFBPPR6076 ---- Plant proteins ---- Putative Pol polyprotein from transposon element Bs1 Source.38: DFBPPR6080 ---- Plant proteins ---- Zein-alpha 19A2 Source.39: DFBPPR6081 ---- Plant proteins ---- Zein-alpha 19B1 Source.40: DFBPPR6082 ---- Plant proteins ---- Zein-alpha 19D1 Source.41: DFBPPR6085 ---- Plant proteins ---- Zein-alpha A30 Source.42: DFBPPR6088 ---- Plant proteins ---- Zein-alpha ZG99 Source.43: DFBPPR6093 ---- Plant proteins ---- Zein-alpha 19C1 Source.44: DFBPPR6094 ---- Plant proteins ---- Zein-alpha PZ19.1 Source.45: DFBPPR6095 ---- Plant proteins ---- Zein-alpha A20 Source.46: DFBPPR6096 ---- Plant proteins ---- Zein-alpha GZ19AB11 Source.47: DFBPPR6101 ---- Plant proteins ---- Zein-alpha M6 Source.48: DFBPPR6110 ---- Plant proteins ---- Zein-alpha Z4 Source.49: DFBPPR6484 ---- Plant proteins ---- Cytochrome P450 97B1, chloroplastic Source.50: DFBPPR6731 ---- Plant proteins ---- Plasma membrane ATPase Source.51: DFBPPR6775 ---- Plant proteins ---- NADH-ubiquinone oxidoreductase chain 4 Source.52: DFBPPR6830 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.53: DFBPPR6840 ---- Plant proteins ---- 30S ribosomal protein S18, chloroplastic Source.54: DFBPPR7080 ---- Plant proteins ---- Ent-kaurenoic acid oxidase 1 Source.55: DFBPPR7208 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.56: DFBPPR7308 ---- Plant proteins ---- 30S ribosomal protein S18, chloroplastic Source.57: DFBPPR7400 ---- Plant proteins ---- Myrosinase Source.58: DFBPPR8363 ---- Plant proteins ---- 13S globulin seed storage protein 1 Source.59: DFBPPR8462 ---- Plant proteins ---- 30S ribosomal protein S18, chloroplastic Source.60: DFBPPR8466 ---- Plant proteins ---- DNA-directed RNA polymerase subunit alpha Source.61: DFBPPR16104 ---- Animal proteins ---- Retinal guanylyl cyclase 1 Source.62: DFBPPR16255 ---- Animal proteins ---- Frizzled-6 Source.63: DFBPPR16736 ---- Animal proteins ---- WD repeat-containing protein 46 Source.64: DFBPPR17104 ---- Animal proteins ---- Endoplasmic reticulum chaperone BiP Source.65: DFBPPR17527 ---- Animal proteins ---- Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Source.66: DFBPPR17845 ---- Animal proteins ---- Solute carrier family 2, facilitated glucose transporter member 5 Source.67: DFBPPR18082 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A activator Source.68: DFBPPR18085 ---- Animal proteins ---- Staphylococcal nuclease domain-containing protein 1 Source.69: DFBPPR18235 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-5 Source.70: DFBPPR18455 ---- Animal proteins ---- Sodium-dependent phosphate transport protein 2B Source.71: DFBPPR18830 ---- Animal proteins ---- Gamma-secretase subunit PEN-2 Source.72: DFBPPR19226 ---- Animal proteins ---- Caseinolytic peptidase B protein homolog Source.73: DFBPPR19684 ---- Animal proteins ---- Urotensin-2 receptor Source.74: DFBPPR20419 ---- Animal proteins ---- Solute carrier family 52, riboflavin transporter, member 3 Source.75: DFBPPR20426 ---- Animal proteins ---- Thimet oligopeptidase Source.76: DFBPPR20463 ---- Animal proteins ---- Transmembrane protein 79 Source.77: DFBPPR20994 ---- Animal proteins ---- Transmembrane 9 superfamily member 1 Source.78: DFBPPR21334 ---- Animal proteins ---- LisH domain-containing protein ARMC9 Source.79: DFBPPR21643 ---- Animal proteins ---- Diacylglycerol O-acyltransferase 2-like protein 6 Source.80: DFBPPR21714 ---- Animal proteins ---- tRNA (guanine(10)-N2)-methyltransferase homolog Source.81: DFBPPR21921 ---- Animal proteins ---- AP-5 complex subunit beta-1 Source.82: DFBPPR8716 ---- Animal proteins ---- Thyroid peroxidase Source.83: DFBPPR8732 ---- Animal proteins ---- Dihydropyrimidine dehydrogenase [NADP(+)] Source.84: DFBPPR9135 ---- Animal proteins ---- mRNA-decapping enzyme 1A Source.85: DFBPPR9600 ---- Animal proteins ---- P2Y purinoceptor 2 Source.86: DFBPPR9764 ---- Animal proteins ---- Cationic amino acid transporter 2 Source.87: DFBPPR10314 ---- Animal proteins ---- Angiotensin-converting enzyme Source.88: DFBPPR10542 ---- Animal proteins ---- Erythroid transcription factor Source.89: DFBPPR11286 ---- Animal proteins ---- Protein wntless homolog Source.90: DFBPPR11553 ---- Animal proteins ---- V-type proton ATPase 116 kDa subunit a1 Source.91: DFBPPR11805 ---- Animal proteins ---- Tudor domain-containing protein 3 Source.92: DFBPPR11959 ---- Animal proteins ---- Deubiquitinase OTUD6B Source.93: DFBPPR12099 ---- Animal proteins ---- tRNA (guanine(10)-N2)-methyltransferase homolog Source.94: DFBPPR12267 ---- Animal proteins ---- Angiotensin-converting enzyme Source.95: DFBPPR12282 ---- Animal proteins ---- Cytochrome P450 1A1 Source.96: DFBPPR12456 ---- Animal proteins ---- Sodium/potassium-transporting ATPase subunit alpha-1 Source.97: DFBPPR12459 ---- Animal proteins ---- Cytochrome P450 4A6 Source.98: DFBPPR12466 ---- Animal proteins ---- Cytochrome P450 4A7 Source.99: DFBPPR12479 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A activator Source.100: DFBPPR12520 ---- Animal proteins ---- Cytochrome P450 4A4 Source.101: DFBPPR12606 ---- Animal proteins ---- Cytochrome P450 4A5 Source.102: DFBPPR12921 ---- Animal proteins ---- Cytochrome P450 2C30 Source.103: DFBPPR13522 ---- Animal proteins ---- Calcitonin receptor-stimulating peptide 2 Source.104: DFBPPR13768 ---- Animal proteins ---- Solute carrier family 2, facilitated glucose transporter member 5 Source.105: DFBPPR13865 ---- Animal proteins ---- C-C chemokine receptor type 9 Source.106: DFBPPR14204 ---- Marine protein ---- Riboflavin transporter 2 Source.107: DFBPPR14540 ---- Marine protein ---- Cytochrome P450 1A1 Source.108: DFBPPR14544 ---- Marine protein ---- Cytochrome P450 1A3 Source.109: DFBPPR15137 ---- Microorganism protein ---- Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase Source.110: DFBPPR15288 ---- Microorganism protein ---- Serine/threonine-protein phosphatase 2A activator 2 Source.111: DFBPPR15299 ---- Microorganism protein ---- Histone chaperone RTT106 Source.112: DFBPPR15488 ---- Microorganism protein ---- Multiple RNA-binding domain-containing protein 1 Source.113: DFBPPR7816 ---- Plant protein ---- DNA-directed RNA polymerase subunit alpha Source.114: DFBPPR7858 ---- Plant protein ---- 30S ribosomal protein S18, chloroplastic Source.115: DFBPPR8098 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 6, chloroplastic
Link-research Inductive analysis	Link 1: DFBPANOX1025----Corn----Corn gluten meal

Biological/Functional activity & target protein

Antioxidative activity	Bioactivity was determined using preventing hepatic fibrosis assay.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Corn peptides and zein peptides were prepared from defatted corn gluten meal and zein, respectively by proteolysis with alcalase, an alkaline protease.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Li, H.-M., Hu, X.I.N., Guo, P., Fu, P., Xu, L.I., Zhang, X.-Z. Antioxidant Properties and Possible Mode of Action of Corn Protein Peptides and Zein Peptides. Journal of Food Biochemistry. 2010, 34, 44-60. DOI: 10.1111/j.1745-4514.2009.00292.x
Other literature(s)	[1] Zou, T.B., et al., The Structure-Activity Relationship of the Antioxidant Peptides from Natural Proteins. Molecules, 2016. 21(1): p. 72. [2] Wang, C., et al., High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry. J Food Drug Anal, 2016. 24(1): p. 95-104.
PubDate	2010