Peptide MTEEY showed potent antioxidant activity according to Ferric reducing antioxidant power (FRAP) (311.80 μmol FeSO4 mg−1), Fe2+ chelation (1.01 μmol Na2EDTA mg−1) assays, and also in DPPH (662.13 μmol Trolox per mg of dry peptide) and ABTS (302.73 μmol Trolox mg−1) radical scavenging assays. As shown in Table 1.
Table 1. Antioxidant and metal chelating activities of the peptide MPACGSS present in the common beans (Phaseolus vulgaris L) non-digesti- ble fractiona
Peptide
| FRAPb
| TEAC (ABTS)c
| TEAC (DPPH)c
| Chelating activityd
| MTEEY | 311.80 ± 18.66b
| 302.73 ± 10.60b
| 662.13 ± 0.28b
| 1.01 ± 0.089c
| Different letters within columns mean significant difference (p<0.05) as determined by Tukey test. a The results are expressed as mean ± standard deviation of three independent experiments. The peptides represent approximately 70% of the total detected protein in the non-digestible fraction of common beans. b Expressed as μmol FeSO4 per mg of dry peptide. c Expressed as μmol Trolox per mg of dry peptide. d Expressed as μmol Na2EDTA per mg of dry peptide. | |