DFBP ID - DFBPANOX0527(Antioxidative peptide)

DFBP ID DFBPANOX0527 Peptide sequence IRGCRL Type Native peptide Peptide/Function name Antioxidative peptide

Function-activity relationship

Main bioactivity Antioxidative activity Otheir bioactivity Cytotoxic activity [D1], Multifunctional activity [D2]

Calculated physicochemical properties

Three-letter amino acid Ile-Arg-Gly-Cys-Arg-Leu Single-letter amino acid IRGCRL Peptide length 6 Peptide mass Experimental mass Theoretical mass 716.6 Da 716.89 Da c Net charge 0.00 c Isoelectric point (pI) 10.89 c IC50 N.D pIC50 N.D GRAVY 0.2333 c Hydrophilic residue ratio 50% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Hen Precursor protein Egg-white lysozyme Residue position f(124-129) Precursor protein(s) search Source.1: DFBPPR9965 ---- Animal proteins ---- Lysozyme C Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity Oxygen radical absorbance capacity-fluorescein (ORAC-FL) assay The peptide presented antioxidant activity with values of 0.313 ± 0.029 umol Trolox/umol peptide. Thiobarbituric acid reactive substances (TBARS) in zebrafish larvae The peptide was found to efficiently inhibit lipid perosidation (61.0 % inhibition of lipid peroxidation). Toxicity in the Zebrafish model (Danio rerio) The peptide was not toxic in zebrafish eggs and larvae at concentrations lower than 50 ug/ml. Concentrations higher than 50 ug/ml was toxic for both zebrafish eggs and larvae. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation Enzymatic hydrolysis Enzyme(s)/starter culture Lysozyme was hydrolyzed in situ with pepsin to generate positive charged peptides.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: In the zebrafish larvae test, this peptide does not present toxicity at a concentration of 50 ug/ml for zebrafish eggs. However, concentration higher than 50 ug/ml of peptide was cytotoxic to zebrafish egg after 24 hours of incubation. Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

DFBP [D1] DFBPCYPE0005 [D2] DFBPMUFU0664

BIOPEP-UWM [D3] 9134, 9139

APD [D4] -

BioPepDB [D5] -

MBPDB [D6] -

Reference(s)

Primary literature Carrillo, W., Gómez-Ruiz, J.A., Miralles, B., Ramos, M., Barrio, D., Recio, I. Identification of antioxidant peptides of hen egg-white lysozyme and evaluation of inhibition of lipid peroxidation and cytotoxicity in the Zebrafish model. European Food Research and Technology. 2016, 242, 1777-85. DOI: 10.1007/s00217-016-2677-1 Other literature(s) N.D PubDate 2016