Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0552(Antioxidative peptide)

DFBP ID	DFBPANOX0552
Peptide sequence	ADGF
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ala-Asp-Gly-Phe

Single-letter amino acid

ADGF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
408.16 Da	408.40 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.13 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hazelnut (Corylus heterophylla Fisch)
Precursor protein	Hazelnut protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1023 ---- Plant proteins ---- Protein disulfide isomerase-like 1-1 Source.2: DFBPPR1551 ---- Plant proteins ---- Syn-pimara-7,15-diene synthase Source.3: DFBPPR1764 ---- Plant proteins ---- B3 domain-containing protein VP1 Source.4: DFBPPR2007 ---- Plant proteins ---- Plasma membrane ATPase Source.5: DFBPPR2109 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase CMT1 Source.6: DFBPPR2547 ---- Plant proteins ---- Ribose-phosphate pyrophosphokinase 3, chloroplastic Source.7: DFBPPR2549 ---- Plant proteins ---- Heat stress transcription factor C-2a Source.8: DFBPPR2571 ---- Plant proteins ---- Protochlorophyllide reductase B, chloroplastic Source.9: DFBPPR2685 ---- Plant proteins ---- Homogentisate 1,2-dioxygenase Source.10: DFBPPR2759 ---- Plant proteins ---- Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOGL9 Source.11: DFBPPR3121 ---- Plant proteins ---- Endoglucanase 23 Source.12: DFBPPR3155 ---- Plant proteins ---- Acyl transferase 1 Source.13: DFBPPR3408 ---- Plant proteins ---- Coatomer subunit delta-1 Source.14: DFBPPR3454 ---- Plant proteins ---- Acyl-[acyl-carrier-protein] desaturase 7, chloroplastic Source.15: DFBPPR3675 ---- Plant proteins ---- Neutral/alkaline invertase 3, chloroplastic Source.16: DFBPPR3809 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 52A Source.17: DFBPPR4105 ---- Plant proteins ---- Squamosa promoter-binding-like protein 15 Source.18: DFBPPR4493 ---- Plant proteins ---- Putative L-cysteine desulfhydrase 1 Source.19: DFBPPR4499 ---- Plant proteins ---- Hydroxycinnamoyltransferase 2 Source.20: DFBPPR4506 ---- Plant proteins ---- Putative L-cysteine desulfhydrase 2 Source.21: DFBPPR4517 ---- Plant proteins ---- Protein MEI2-like 3 Source.22: DFBPPR4647 ---- Plant proteins ---- BURP domain-containing protein 12 Source.23: DFBPPR4760 ---- Plant proteins ---- Putative zinc finger CCCH domain-containing protein 21 Source.24: DFBPPR4933 ---- Plant proteins ---- Cinnamyl alcohol dehydrogenase 7 Source.25: DFBPPR4934 ---- Plant proteins ---- U-box domain-containing protein 70 Source.26: DFBPPR6153 ---- Plant proteins ---- Ninja-family protein 8 Source.27: DFBPPR6154 ---- Plant proteins ---- Ninja-family protein 7 Source.28: DFBPPR6245 ---- Plant proteins ---- Glycine dehydrogenase (decarboxylating), mitochondrial Source.29: DFBPPR6350 ---- Plant proteins ---- Galactoside 2-alpha-L-fucosyltransferase Source.30: DFBPPR6354 ---- Plant proteins ---- Protochlorophyllide reductase, chloroplastic Source.31: DFBPPR6377 ---- Plant proteins ---- Lectin Source.32: DFBPPR6731 ---- Plant proteins ---- Plasma membrane ATPase Source.33: DFBPPR7138 ---- Plant proteins ---- Protochlorophyllide reductase B, chloroplastic Source.34: DFBPPR7655 ---- Milk proteins ---- Protein FAM13A Source.35: DFBPPR8526 ---- Milk proteins ---- Protein FAM13A Source.36: DFBPPR16476 ---- Animal proteins ---- Thrombomodulin Source.37: DFBPPR16641 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.38: DFBPPR17423 ---- Animal proteins ---- Furin Source.39: DFBPPR18073 ---- Animal proteins ---- Endoplasmic reticulum aminopeptidase 2 Source.40: DFBPPR18231 ---- Animal proteins ---- Transcription factor jun-B Source.41: DFBPPR18236 ---- Animal proteins ---- Latent-transforming growth factor beta-binding protein 2 Source.42: DFBPPR18319 ---- Animal proteins ---- MMS19 nucleotide excision repair protein homolog Source.43: DFBPPR18439 ---- Animal proteins ---- Retinol dehydrogenase 12 Source.44: DFBPPR18703 ---- Animal proteins ---- Thialysine N-epsilon-acetyltransferase Source.45: DFBPPR18730 ---- Animal proteins ---- Eyes absent homolog 2 Source.46: DFBPPR18952 ---- Animal proteins ---- Serotransferrin Source.47: DFBPPR19026 ---- Animal proteins ---- Long-chain-fatty-acid--CoA ligase ACSBG1 Source.48: DFBPPR19202 ---- Animal proteins ---- Zinc finger protein 639 Source.49: DFBPPR19340 ---- Animal proteins ---- Aryl hydrocarbon receptor nuclear translocator Source.50: DFBPPR20030 ---- Animal proteins ---- Calumenin Source.51: DFBPPR20451 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.52: DFBPPR21115 ---- Animal proteins ---- Ras-related and estrogen-regulated growth inhibitor-like protein Source.53: DFBPPR21509 ---- Animal proteins ---- Myoneurin Source.54: DFBPPR21523 ---- Animal proteins ---- tRNA 2'-phosphotransferase 1 Source.55: DFBPPR22237 ---- Animal proteins ---- Spermatogenesis-associated protein 5-like protein 1 Source.56: DFBPPR9359 ---- Animal proteins ---- Thialysine N-epsilon-acetyltransferase Source.57: DFBPPR9928 ---- Animal proteins ---- Galectin-2 Source.58: DFBPPR9997 ---- Animal proteins ---- Aryl hydrocarbon receptor nuclear translocator-like protein 1 Source.59: DFBPPR10045 ---- Animal proteins ---- Albumin Source.60: DFBPPR10172 ---- Animal proteins ---- Basic helix-loop-helix transcription factor scleraxis Source.61: DFBPPR10243 ---- Animal proteins ---- Core histone macro-H2A.1 Source.62: DFBPPR10277 ---- Animal proteins ---- Nitric oxide synthase, inducible Source.63: DFBPPR10518 ---- Animal proteins ---- Neural cell adhesion molecule 1 Source.64: DFBPPR10744 ---- Animal proteins ---- Troponin C, skeletal muscle Source.65: DFBPPR11039 ---- Animal proteins ---- Neurexin-3 Source.66: DFBPPR11106 ---- Animal proteins ---- Aryl hydrocarbon receptor nuclear translocator-like protein 2 Source.67: DFBPPR11146 ---- Animal proteins ---- Formin Source.68: DFBPPR11277 ---- Animal proteins ---- Abasic site processing protein HMCES Source.69: DFBPPR11293 ---- Animal proteins ---- RIMS-binding protein 2 Source.70: DFBPPR11320 ---- Animal proteins ---- Nucleoporin NUP42 Source.71: DFBPPR11399 ---- Animal proteins ---- Probable glutamate--tRNA ligase, mitochondrial Source.72: DFBPPR11855 ---- Animal proteins ---- G-protein coupled receptor-associated protein LMBRD2 Source.73: DFBPPR11918 ---- Animal proteins ---- Nucleoside diphosphate-linked moiety X motif 19 Source.74: DFBPPR11978 ---- Animal proteins ---- ER membrane protein complex subunit 1 Source.75: DFBPPR12365 ---- Animal proteins ---- Sucrase-isomaltase, intestinal Source.76: DFBPPR12545 ---- Animal proteins ---- Galectin-3 Source.77: DFBPPR12773 ---- Animal proteins ---- Aryl hydrocarbon receptor nuclear translocator Source.78: DFBPPR13165 ---- Animal proteins ---- Aryl hydrocarbon receptor nuclear translocator-like protein 1 Source.79: DFBPPR14539 ---- Marine protein ---- Aryl hydrocarbon receptor nuclear translocator Source.80: DFBPPR14892 ---- Microorganism protein ---- Histone-lysine N-methyltransferase, H3 lysine-4 specific Source.81: DFBPPR14901 ---- Microorganism protein ---- Plasma membrane ATPase Source.82: DFBPPR7954 ---- Plant protein ---- Favin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The synthetic peptide ADGF showed excellent DPPH radical scavenging ability and ABTS radical scavenging activity in a dose-dependent manner (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hazelnut protein was hydrolyzed with Alcalase 2.4L at a constant temperature of 54 °C and pH of 8.0 in a heated water bath for 2.5 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptides isolated from wild hazelnut protein hydrolysates may be used as potential antioxidants in the food and drug industries.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Liu, C., Ren, D., Li, J., Fang, L., Wang, J., Liu, J., et al. Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates. Journal of Functional Foods. 2018, 42, 203-15. DOI: 10.1016/j.jff.2017.12.003
Other literature(s)	N.D
PubDate	2018