Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0553(Antioxidative peptide)

DFBP ID	DFBPANOX0553
Peptide sequence	AGGF
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ala-Gly-Gly-Phe

Single-letter amino acid

AGGF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
350.16 Da	350.37 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.9500^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hazelnut (Corylus heterophylla Fisch)
Precursor protein	Hazelnut protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0858 ---- Plant proteins ---- Bidirectional sugar transporter SWEET11 Source.2: DFBPPR1069 ---- Plant proteins ---- Cinnamoyl-CoA reductase 1 Source.3: DFBPPR1363 ---- Plant proteins ---- Protein YELLOW LEAF 1, choloroplastic Source.4: DFBPPR1421 ---- Plant proteins ---- GDP-mannose 3,5-epimerase 1 Source.5: DFBPPR1467 ---- Plant proteins ---- Chaperone protein ClpD1, chloroplastic Source.6: DFBPPR1487 ---- Plant proteins ---- Beta-1,2-xylosyltransferease XAX1 Source.7: DFBPPR1683 ---- Plant proteins ---- Probable acetolactate synthase 2, chloroplastic Source.8: DFBPPR2110 ---- Plant proteins ---- Sugar transport protein MST4 Source.9: DFBPPR2253 ---- Plant proteins ---- Probable methylenetetrahydrofolate reductase Source.10: DFBPPR2366 ---- Plant proteins ---- Protein NODULATION SIGNALING PATHWAY 2 Source.11: DFBPPR2393 ---- Plant proteins ---- 4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic Source.12: DFBPPR2559 ---- Plant proteins ---- Two-component response regulator ORR27 Source.13: DFBPPR2573 ---- Plant proteins ---- E3 ubiquitin-protein ligase Os03g0188200 Source.14: DFBPPR2592 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase CMT2 Source.15: DFBPPR2773 ---- Plant proteins ---- Tripeptidyl-peptidase 2 Source.16: DFBPPR2778 ---- Plant proteins ---- PHD finger protein PERSISTENT TAPETAL CELL 1 Source.17: DFBPPR2891 ---- Plant proteins ---- GDP-mannose 3,5-epimerase 2 Source.18: DFBPPR3125 ---- Plant proteins ---- Putative alpha-L-fucosidase 1 Source.19: DFBPPR3163 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX32 Source.20: DFBPPR3198 ---- Plant proteins ---- Probable protein phosphatase 2C 59 Source.21: DFBPPR3229 ---- Plant proteins ---- Transcription factor TGAL7 Source.22: DFBPPR3252 ---- Plant proteins ---- Probable protein phosphatase 2C 70 Source.23: DFBPPR3254 ---- Plant proteins ---- Probable protein phosphatase 2C 10 Source.24: DFBPPR3363 ---- Plant proteins ---- Beta-galactosidase 2 Source.25: DFBPPR3380 ---- Plant proteins ---- Vacuolar iron transporter homolog 1 Source.26: DFBPPR3444 ---- Plant proteins ---- Vacuolar iron transporter homolog 3 Source.27: DFBPPR3558 ---- Plant proteins ---- Probable protein phosphatase 2C 45 Source.28: DFBPPR3713 ---- Plant proteins ---- Probable NADH kinase Source.29: DFBPPR3794 ---- Plant proteins ---- UDP-D-apiose/UDP-D-xylose synthase Source.30: DFBPPR4015 ---- Plant proteins ---- GDT1-like protein 3 Source.31: DFBPPR4022 ---- Plant proteins ---- Protein TIFY 11f Source.32: DFBPPR4052 ---- Plant proteins ---- Beta-galactosidase 9 Source.33: DFBPPR4058 ---- Plant proteins ---- Probable protein phosphatase 2C 11 Source.34: DFBPPR4162 ---- Plant proteins ---- Potassium transporter 6 Source.35: DFBPPR4384 ---- Plant proteins ---- Putative WUSCHEL-related homeobox 2 Source.36: DFBPPR4386 ---- Plant proteins ---- WUSCHEL-related homeobox 5 Source.37: DFBPPR4387 ---- Plant proteins ---- Dof zinc finger protein 5 Source.38: DFBPPR4526 ---- Plant proteins ---- BURP domain-containing protein 14 Source.39: DFBPPR4553 ---- Plant proteins ---- Phospholipase A1-II 7 Source.40: DFBPPR4826 ---- Plant proteins ---- Probable protein transport Sec1a Source.41: DFBPPR4891 ---- Plant proteins ---- Isoamylase 1, chloroplastic Source.42: DFBPPR4983 ---- Plant proteins ---- Protein SRC2 Source.43: DFBPPR5231 ---- Plant proteins ---- Casparian strip membrane protein 5 Source.44: DFBPPR5415 ---- Plant proteins ---- Eudesmanediol synthase Source.45: DFBPPR5595 ---- Plant proteins ---- Calcium sensing receptor, chloroplastic Source.46: DFBPPR5608 ---- Plant proteins ---- Dolabradiene synthase KSL4, chloroplastic Source.47: DFBPPR5634 ---- Plant proteins ---- Eudesmanediol synthase Source.48: DFBPPR6824 ---- Plant proteins ---- Protein RAFTIN 1B Source.49: DFBPPR7042 ---- Plant proteins ---- Glucan endo-1,3-beta-glucosidase GII Source.50: DFBPPR7136 ---- Plant proteins ---- Glucan endo-1,3-beta-glucosidase GIV Source.51: DFBPPR7176 ---- Plant proteins ---- Glucan endo-1,3-beta-glucosidase GV Source.52: DFBPPR7204 ---- Plant proteins ---- Thiol protease aleurain Source.53: DFBPPR8391 ---- Plant proteins ---- Oleosin Ara h 15.0101 Source.54: DFBPPR16047 ---- Animal proteins ---- Adenylate cyclase type 5 Source.55: DFBPPR16128 ---- Animal proteins ---- Cubilin Source.56: DFBPPR16182 ---- Animal proteins ---- Heat shock 70 kDa protein 1 Source.57: DFBPPR16222 ---- Animal proteins ---- Sodium- and chloride-dependent taurine transporter Source.58: DFBPPR16314 ---- Animal proteins ---- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Source.59: DFBPPR16326 ---- Animal proteins ---- Desmin Source.60: DFBPPR16634 ---- Animal proteins ---- Zinc transporter SLC39A7 Source.61: DFBPPR16842 ---- Animal proteins ---- Collagen alpha-1(II) chain Source.62: DFBPPR16899 ---- Animal proteins ---- Heat shock 70 kDa protein 1A Source.63: DFBPPR17008 ---- Animal proteins ---- Prolyl endopeptidase FAP Source.64: DFBPPR17038 ---- Animal proteins ---- NAD(P) transhydrogenase, mitochondrial Source.65: DFBPPR17691 ---- Animal proteins ---- Integrin alpha-L Source.66: DFBPPR18390 ---- Animal proteins ---- Phosphoglycerate kinase 1 Source.67: DFBPPR18781 ---- Animal proteins ---- Exosome complex component RRP4 Source.68: DFBPPR19307 ---- Animal proteins ---- Microprocessor complex subunit DGCR8 Source.69: DFBPPR19342 ---- Animal proteins ---- Calcium load-activated calcium channel Source.70: DFBPPR19449 ---- Animal proteins ---- SCO-spondin Source.71: DFBPPR19516 ---- Animal proteins ---- Protein phosphatase 1L Source.72: DFBPPR19624 ---- Animal proteins ---- Keratin, type II cytoskeletal 5 Source.73: DFBPPR19670 ---- Animal proteins ---- SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 Source.74: DFBPPR19778 ---- Animal proteins ---- Sodium- and chloride-dependent taurine transporter Source.75: DFBPPR19862 ---- Animal proteins ---- Splicing factor 3B subunit 3 Source.76: DFBPPR19977 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX27 Source.77: DFBPPR21025 ---- Animal proteins ---- Radial spoke head protein 3 homolog Source.78: DFBPPR21191 ---- Animal proteins ---- RAS protein activator like-3 Source.79: DFBPPR21338 ---- Animal proteins ---- S-adenosylmethionine mitochondrial carrier protein Source.80: DFBPPR21745 ---- Animal proteins ---- Mastermind-like protein 2 Source.81: DFBPPR21932 ---- Animal proteins ---- Keratin, type II cytoskeletal 68 kDa, component IB Source.82: DFBPPR22093 ---- Animal proteins ---- F-box only protein 3 Source.83: DFBPPR8981 ---- Animal proteins ---- Deleted in malignant brain tumors 1 protein Source.84: DFBPPR8990 ---- Animal proteins ---- Phosphoglycerate kinase 1 Source.85: DFBPPR9293 ---- Animal proteins ---- Calcium load-activated calcium channel Source.86: DFBPPR9347 ---- Animal proteins ---- C-reactive protein Source.87: DFBPPR9409 ---- Animal proteins ---- Doublesex- and mab-3-related transcription factor 1 Source.88: DFBPPR9764 ---- Animal proteins ---- Cationic amino acid transporter 2 Source.89: DFBPPR10047 ---- Animal proteins ---- Ovocleidin-116 Source.90: DFBPPR10295 ---- Animal proteins ---- NAD-dependent protein deacylase sirtuin-5, mitochondrial Source.91: DFBPPR10555 ---- Animal proteins ---- Fanconi anemia group M protein Source.92: DFBPPR10628 ---- Animal proteins ---- Nuclear factor NF-kappa-B p100 subunit Source.93: DFBPPR10945 ---- Animal proteins ---- Prosaposin Source.94: DFBPPR11129 ---- Animal proteins ---- Zinc finger protein ZIC 1 Source.95: DFBPPR11347 ---- Animal proteins ---- Neogenin Source.96: DFBPPR11553 ---- Animal proteins ---- V-type proton ATPase 116 kDa subunit a1 Source.97: DFBPPR12008 ---- Animal proteins ---- Keratin, type II cytoskeletal cochleal Source.98: DFBPPR12346 ---- Animal proteins ---- Adenylate cyclase type 5 Source.99: DFBPPR12764 ---- Animal proteins ---- Keratin, type II cytoskeletal 3 Source.100: DFBPPR12862 ---- Animal proteins ---- Tumor necrosis factor-inducible gene 6 protein Source.101: DFBPPR13214 ---- Animal proteins ---- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Source.102: DFBPPR13254 ---- Animal proteins ---- Phosphoglycerate kinase 1 Source.103: DFBPPR13617 ---- Animal proteins ---- NAD(P) transhydrogenase, mitochondrial Source.104: DFBPPR14624 ---- Marine protein ---- Heat shock cognate 70 kDa protein Source.105: DFBPPR14996 ---- Microorganism protein ---- Homocysteine/cysteine synthase Source.106: DFBPPR15128 ---- Microorganism protein ---- Deoxyuridine 5'-triphosphate nucleotidohydrolase Source.107: DFBPPR15412 ---- Microorganism protein ---- DNA repair protein RAD59 Source.108: DFBPPR15765 ---- Microorganism protein ---- Protein FMP52, mitochondrial Source.109: DFBPPR15817 ---- Microorganism protein ---- PTS system sorbose-specific EIIC component Source.110: DFBPPR15879 ---- Microorganism protein ---- Probable DNA polymerase Source.111: DFBPPR7939 ---- Plant protein ---- Peptidyl-prolyl cis-trans isomerase FKBP12
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The synthetic peptide AGGF showed excellent DPPH radical scavenging ability and ABTS radical scavenging activity in a dose-dependent manner (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hazelnut protein was hydrolyzed with Alcalase 2.4L at a constant temperature of 54 °C and pH of 8.0 in a heated water bath for 2.5 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptides isolated from wild hazelnut protein hydrolysates may be used as potential antioxidants in the food and drug industries.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Liu, C., Ren, D., Li, J., Fang, L., Wang, J., Liu, J., et al. Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates. Journal of Functional Foods. 2018, 42, 203-15. DOI: 10.1016/j.jff.2017.12.003
Other literature(s)	N.D
PubDate	2018