Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0556(Antioxidative peptide)

DFBP ID	DFBPANOX0556
Peptide sequence	ETTL
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Thr-Thr-Leu

Single-letter amino acid

ETTL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
462.23 Da	462.49 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.2750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hazelnut (Corylus heterophylla Fisch)
Precursor protein	Hazelnut protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0747 ---- Plant proteins ---- 11S globulin seed storage protein Source.2: DFBPPR0776 ---- Plant proteins ---- 11S globulin Source.3: DFBPPR0844 ---- Plant proteins ---- Jasmonoyl--L-amino acid synthetase GH3.5 Source.4: DFBPPR1079 ---- Plant proteins ---- Hexokinase-7 Source.5: DFBPPR1306 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.6: DFBPPR1385 ---- Plant proteins ---- Glutamine synthetase cytosolic isozyme 1-2 Source.7: DFBPPR1481 ---- Plant proteins ---- Leucine-rich repeat receptor protein kinase MSL1 Source.8: DFBPPR1537 ---- Plant proteins ---- Zinc transporter 8 Source.9: DFBPPR1783 ---- Plant proteins ---- Glucose-6-phosphate isomerase, cytosolic A Source.10: DFBPPR1917 ---- Plant proteins ---- Kinesin-like protein KIN-12A Source.11: DFBPPR1965 ---- Plant proteins ---- DNA polymerase I B, mitochondrial Source.12: DFBPPR1997 ---- Plant proteins ---- Glucose-6-phosphate isomerase, cytosolic B Source.13: DFBPPR2174 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX29 Source.14: DFBPPR2382 ---- Plant proteins ---- Putative cellulose synthase-like protein H3 Source.15: DFBPPR3046 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 35B Source.16: DFBPPR3086 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX33 Source.17: DFBPPR3163 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX32 Source.18: DFBPPR3240 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 35A Source.19: DFBPPR3436 ---- Plant proteins ---- Magnesium transporter MRS2-F Source.20: DFBPPR3794 ---- Plant proteins ---- UDP-D-apiose/UDP-D-xylose synthase Source.21: DFBPPR5118 ---- Plant proteins ---- Trans-cinnamate 4-monooxygenase Source.22: DFBPPR5454 ---- Plant proteins ---- Ferredoxin-dependent glutamate synthase, chloroplastic Source.23: DFBPPR5821 ---- Plant proteins ---- Glucose-6-phosphate isomerase, cytosolic Source.24: DFBPPR5929 ---- Plant proteins ---- Non-symbiotic hemoglobin Source.25: DFBPPR6242 ---- Plant proteins ---- Delta-aminolevulinic acid dehydratase, chloroplastic Source.26: DFBPPR6415 ---- Plant proteins ---- Trans-cinnamate 4-monooxygenase Source.27: DFBPPR6474 ---- Plant proteins ---- Stromal 70 kDa heat shock-related protein, chloroplastic Source.28: DFBPPR6670 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.29: DFBPPR7117 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.30: DFBPPR7157 ---- Plant proteins ---- Non-symbiotic hemoglobin Source.31: DFBPPR7416 ---- Plant proteins ---- Cruciferin CRU4 Source.32: DFBPPR7417 ---- Plant proteins ---- Cruciferin Source.33: DFBPPR7444 ---- Plant proteins ---- Cruciferin BnC2 Source.34: DFBPPR7445 ---- Plant proteins ---- Cruciferin CRU1 Source.35: DFBPPR7446 ---- Plant proteins ---- Cruciferin BnC1 Source.36: DFBPPR7464 ---- Plant proteins ---- Glutamine synthetase, chloroplastic Source.37: DFBPPR7603 ---- Milk proteins ---- Xanthine dehydrogenase/oxidase Source.38: DFBPPR8498 ---- Milk proteins ---- Xanthine dehydrogenase/oxidase Source.39: DFBPPR16125 ---- Animal proteins ---- Heat shock factor protein 4 Source.40: DFBPPR16563 ---- Animal proteins ---- Myosin-16 Source.41: DFBPPR16822 ---- Animal proteins ---- Kininogen-2 Source.42: DFBPPR16830 ---- Animal proteins ---- Kininogen-1 Source.43: DFBPPR16921 ---- Animal proteins ---- Lysosomal alpha-mannosidase Source.44: DFBPPR17350 ---- Animal proteins ---- DNA mismatch repair protein Msh2 Source.45: DFBPPR17623 ---- Animal proteins ---- Ectodysplasin-A Source.46: DFBPPR17624 ---- Animal proteins ---- Ectodysplasin-A Source.47: DFBPPR18122 ---- Animal proteins ---- Microtubule-associated protein 4 Source.48: DFBPPR18779 ---- Animal proteins ---- Mucin-15 Source.49: DFBPPR19074 ---- Animal proteins ---- Lysophosphatidic acid phosphatase type 6 Source.50: DFBPPR19081 ---- Animal proteins ---- Fermitin family homolog 3 Source.51: DFBPPR19147 ---- Animal proteins ---- X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 Source.52: DFBPPR19302 ---- Animal proteins ---- Solute carrier family 2, facilitated glucose transporter member 12 Source.53: DFBPPR19448 ---- Animal proteins ---- Trimethyllysine dioxygenase, mitochondrial Source.54: DFBPPR19783 ---- Animal proteins ---- Syndecan-1 Source.55: DFBPPR20220 ---- Animal proteins ---- Endosome/lysosome-associated apoptosis and autophagy regulator family member 2 Source.56: DFBPPR20329 ---- Animal proteins ---- Meiosis regulator and mRNA stability factor 1 Source.57: DFBPPR20372 ---- Animal proteins ---- WASH complex subunit 3 Source.58: DFBPPR20450 ---- Animal proteins ---- Neurotrophin receptor-interacting factor homolog Source.59: DFBPPR20884 ---- Animal proteins ---- Transmembrane protein 237 Source.60: DFBPPR21194 ---- Animal proteins ---- Thyroxine-binding globulin Source.61: DFBPPR21301 ---- Animal proteins ---- Lymphocyte antigen 6D Source.62: DFBPPR21488 ---- Animal proteins ---- Probable ubiquitin carboxyl-terminal hydrolase MINDY-4 Source.63: DFBPPR21836 ---- Animal proteins ---- Potassium channel regulatory protein Source.64: DFBPPR22032 ---- Animal proteins ---- Armadillo-like helical domain-containing protein 4 Source.65: DFBPPR22149 ---- Animal proteins ---- Putative peptidyl-tRNA hydrolase PTRHD1 Source.66: DFBPPR22535 ---- Animal proteins ---- Protein FAM205C Source.67: DFBPPR8613 ---- Animal proteins ---- Thyroglobulin Source.68: DFBPPR8681 ---- Animal proteins ---- Toll-like receptor 9 Source.69: DFBPPR8767 ---- Animal proteins ---- Lutropin-choriogonadotropic hormone receptor Source.70: DFBPPR8945 ---- Animal proteins ---- Coagulation factor V Source.71: DFBPPR9686 ---- Animal proteins ---- Fatty acid-binding protein, heart Source.72: DFBPPR9983 ---- Animal proteins ---- Fibrinogen alpha chain Source.73: DFBPPR10135 ---- Animal proteins ---- Serine/threonine-protein kinase B-raf Source.74: DFBPPR10150 ---- Animal proteins ---- Tenascin Source.75: DFBPPR10268 ---- Animal proteins ---- CD166 antigen Source.76: DFBPPR10273 ---- Animal proteins ---- Bone morphogenetic protein 4 Source.77: DFBPPR10310 ---- Animal proteins ---- Xanthine dehydrogenase/oxidase Source.78: DFBPPR10361 ---- Animal proteins ---- Protein HIRA Source.79: DFBPPR10422 ---- Animal proteins ---- Collagen alpha-1(XII) chain Source.80: DFBPPR10512 ---- Animal proteins ---- Collagen alpha-1(XIV) chain Source.81: DFBPPR11001 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit beta-3 Source.82: DFBPPR11168 ---- Animal proteins ---- Trimethyllysine dioxygenase, mitochondrial Source.83: DFBPPR12290 ---- Animal proteins ---- Ryanodine receptor 2 Source.84: DFBPPR12571 ---- Animal proteins ---- Inositol hexakisphosphate kinase 2 Source.85: DFBPPR13820 ---- Animal proteins ---- Lutropin-choriogonadotropic hormone receptor Source.86: DFBPPR13860 ---- Animal proteins ---- Thyroxine-binding globulin Source.87: DFBPPR14211 ---- Marine protein ---- WASH complex subunit 3 Source.88: DFBPPR14520 ---- Marine protein ---- Uncharacterized protein ycf23 Source.89: DFBPPR14575 ---- Marine protein ---- Cellular tumor antigen p53 Source.90: DFBPPR14670 ---- Marine protein ---- Fatty acid-binding protein, heart Source.91: DFBPPR14808 ---- Marine protein ---- Pseudohemocyanin-1 Source.92: DFBPPR14898 ---- Microorganism protein ---- Transcription factor IIIB 70 kDa subunit Source.93: DFBPPR15085 ---- Microorganism protein ---- Glucose-6-phosphate isomerase Source.94: DFBPPR15105 ---- Microorganism protein ---- Arginase Source.95: DFBPPR15181 ---- Microorganism protein ---- Nitrogen permease regulator 3 Source.96: DFBPPR15219 ---- Microorganism protein ---- Chromatin structure-remodeling complex subunit SFH1 Source.97: DFBPPR15550 ---- Microorganism protein ---- COPII coat assembly protein SEC16 Source.98: DFBPPR15615 ---- Microorganism protein ---- Probable transporter MCH1 Source.99: DFBPPR15634 ---- Microorganism protein ---- Hsp70 nucleotide exchange factor FES1 Source.100: DFBPPR15644 ---- Microorganism protein ---- Cytoplasmic dynein intermediate light chain DYN3 Source.101: DFBPPR15733 ---- Microorganism protein ---- J protein JJJ2 Source.102: DFBPPR15752 ---- Microorganism protein ---- Protein HRI1 Source.103: DFBPPR15767 ---- Microorganism protein ---- Protein LOT5 Source.104: DFBPPR15775 ---- Microorganism protein ---- Increased recombination centers protein 6 Source.105: DFBPPR7752 ---- Plant protein ---- Trans-cinnamate 4-monooxygenase Source.106: DFBPPR7776 ---- Plant protein ---- Beta-sesquiphellandrene synthase Source.107: DFBPPR8081 ---- Plant protein ---- Glutamine synthetase N-1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The synthetic peptide ETTL showed excellent DPPH radical scavenging ability and ABTS radical scavenging activity in a dose-dependent manner (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hazelnut protein was hydrolyzed with Alcalase 2.4L at a constant temperature of 54 °C and pH of 8.0 in a heated water bath for 2.5 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptides isolated from wild hazelnut protein hydrolysates may be used as potential antioxidants in the food and drug industries.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Liu, C., Ren, D., Li, J., Fang, L., Wang, J., Liu, J., et al. Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates. Journal of Functional Foods. 2018, 42, 203-15. DOI: 10.1016/j.jff.2017.12.003
Other literature(s)	N.D
PubDate	2018