Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0587(Antioxidative peptide)

DFBP ID	DFBPANOX0587
Peptide sequence	YGGFM
Type	Native peptide
Peptide/Function name	Antioxidative peptide, Methionine-enkephalin

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Gly-Gly-Phe-Met

Single-letter amino acid

YGGFM

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	573.67 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.5200^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	The pituitary gland of sheep^[1]
Precursor protein	β-Lipotropin^[1]
Residue position	f(61-65)^[1]
Precursor protein(s) search	Source.1: DFBPPR16803 ---- Animal proteins ---- Pro-opiomelanocortin Source.2: DFBPPR16933 ---- Animal proteins ---- Proenkephalin-A Source.3: DFBPPR8756 ---- Animal proteins ---- Pro-opiomelanocortin Source.4: DFBPPR13531 ---- Animal proteins ---- Pro-opiomelanocortin Source.5: DFBPPR13983 ---- Animal proteins ---- Pro-opiomelanocortin-1 Source.6: DFBPPR13984 ---- Animal proteins ---- Pro-opiomelanocortin-2 Source.7: DFBPPR14230 ---- Marine protein ---- Pro-opiomelanocortin Source.8: DFBPPR14261 ---- Marine protein ---- Beta-endorphin-2 Source.9: DFBPPR14262 ---- Marine protein ---- Pro-opiomelanocortin Source.10: DFBPPR14543 ---- Marine protein ---- Pro-opiomelanocortin A Source.11: DFBPPR14549 ---- Marine protein ---- Pro-opiomelanocortin B
Link-research Inductive analysis	Link 1: DFBPANOX0586----The pituitary gland of pig^[1]----β-Lipotropin^[1]

Biological/Functional activity & target protein

Antioxidative activity	The peptide was potential antioxidant peptide (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCSC)C(=O)O

Preparation method

Mode of preparation	Hydrolysis without enzyme^[1]
Enzyme(s)/starter culture	The pig brains were hydrolyzed with 5.5 N HCl at 110 ℃ for 20 h ^[1].

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Methionine-enkephalin was also found in pig^[1].

Database cross-references

DFBP

[D1]	DFBPOPIO0050, DFBPOPIO0048
[D2]	DFBPMUFU0666

BIOPEP-UWM

[D3]

2877, 7853, 8123, 9522

APD

[D4]

BioPepDB

[D5]

MBPDB

[D6]

Reference(s)

Primary literature	Moosmann B, Behl C. Secretory peptide hormones are biochemical antioxidants: structure-activity relationship. Mol Pharmacol. 2002 Feb;61(2):260-8. PMID: 11809849 DOI: 10.1124/mol.61.2.260
Other literature(s)	[1] Hughes J, Smith T W, Kosterlitz H W, et al. Identification of two related pentapeptides from the brain with potent opiate agonist activity.[J]. Nature, 1975, 258(5536):577-580.
PubDate	2002