Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0588(Antioxidative peptide)

DFBP ID	DFBPANOX0588
Peptide sequence	YGGFL
Type	Native peptide
Peptide/Function name	Antioxidative peptide, Leucine-enkephalin

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Gly-Gly-Phe-Leu

Single-letter amino acid

YGGFL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	555.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.9000^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig brain^[1]
Precursor protein	Pituitary gland^[1]
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2682 ---- Plant proteins ---- Beta-glucosidase 30 Source.2: DFBPPR3043 ---- Plant proteins ---- Beta-glucosidase 24 Source.3: DFBPPR3109 ---- Plant proteins ---- Beta-glucosidase 28 Source.4: DFBPPR3173 ---- Plant proteins ---- Beta-glucosidase 29 Source.5: DFBPPR5377 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic Source.6: DFBPPR5456 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 2, chloroplastic Source.7: DFBPPR6628 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1a, chloroplastic Source.8: DFBPPR6629 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1d, chloroplastic Source.9: DFBPPR6630 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic Source.10: DFBPPR6631 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1c, chloroplastic Source.11: DFBPPR8451 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase, chloroplastic Source.12: DFBPPR16933 ---- Animal proteins ---- Proenkephalin-A Source.13: DFBPPR18352 ---- Animal proteins ---- Proenkephalin-B Source.14: DFBPPR18741 ---- Animal proteins ---- Coatomer subunit beta' Source.15: DFBPPR21616 ---- Animal proteins ---- Olfactomedin-like protein 3 Source.16: DFBPPR8967 ---- Animal proteins ---- Proenkephalin-B
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide was potential antioxidant peptide (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Hydrolysis without enzyme^[1]
Enzyme(s)/starter culture	The pig brains were hydrolyzed with 5.5 N HCl at 110 ℃ for 20 h ^[1].

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPOPIO0052
[D2]	DFBPMUFU0667

BIOPEP-UWM

[D3]

2879, 8122, 9521

APD

[D4]

BioPepDB

[D5]

MBPDB

[D6]

Reference(s)

Primary literature	Moosmann B, Behl C. Secretory peptide hormones are biochemical antioxidants: structure-activity relationship. Mol Pharmacol. 2002 Feb;61(2):260-8. PMID: 11809849 DOI: 10.1124/mol.61.2.260
Other literature(s)	[1] Hughes J, Smith T W, Kosterlitz H W, et al. Identification of two related pentapeptides from the brain with potent opiate agonist activity.[J]. Nature, 1975, 258(5536):577-580.
PubDate	2002