Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0594(Antioxidative peptide)

DFBP ID	DFBPANOX0594
Peptide sequence	FIMGPY
Type	Native peptide
Peptide/Function name	Antioxidative peptide, RCP-I

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	Anticancer activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Phe-Ile-Met-Gly-Pro-Tyr

Single-letter amino acid

FIMGPY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
726.9 Da	726.88 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.9833^c

Hydrophilic residue ratio

83.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Fish
Organism/Source	Skate (Raja porosa)
Precursor protein	Cartilage protein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide RCP-I exhibited good scavenging activities on DPPH^• (EC₅₀ 3.5768 M), HO^• (EC₅₀ 4.1821 M), O₂^•− (EC₅₀ 2.2149 M) and ABTS^•+ (EC₅₀ 1.4307 M), respectively.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCSC)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Skate cartilage protein was hydrolyzed with alcalase and trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide FIMGPY from skate cartilage protein hydrolysate may have applications as functional foods and nutraceuticals for the treatment and prevention of cancer.

Database cross-references

DFBP

[D1]	DFBPANCA0025
[D2]	DFBPMUFU0670

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Pan, X., Zhao, Y.-Q., Hu, F.-Y., Wang, B. Preparation and identification of antioxidant peptides from protein hydrolysate of skate ( Raja porosa ) cartilage. Journal of Functional Foods. 2016, 25, 220-30. DOI: 10.1016/j.jff.2016.06.008
Other literature(s)	[1] Pan X, Zhao Y Q, Hu F Y, et al. Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells[J]. Marine Drugs, 2016, 14(8):153. [2] Chalamaiah M, Yu W, Wu J. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review.[J]. Food Chemistry, 2018, 245:205-222.
PubDate	2016