Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0622(Antioxidative peptide)

DFBP ID	DFBPANOX0622
Peptide sequence	TPSPR
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	Antithrombotic activity ^[D1], α-Amylase inhibitory activity ^[D2], α-Glucosidase inhibitory activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Thr-Pro-Ser-Pro-Arg

Single-letter amino acid

TPSPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	556.61 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.8400^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg white protein
Precursor protein	Ovotransferrin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2938 ---- Plant proteins ---- Kinesin-like protein KIN-10A Source.2: DFBPPR4878 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 10 Source.3: DFBPPR17668 ---- Animal proteins ---- 2'-5'-oligoadenylate synthase 2 Source.4: DFBPPR17924 ---- Animal proteins ---- Sodium-dependent dopamine transporter Source.5: DFBPPR20350 ---- Animal proteins ---- Pinin Source.6: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin Source.7: DFBPPR10393 ---- Animal proteins ---- Vitellogenin-1 Source.8: DFBPPR10610 ---- Animal proteins ---- Denticleless protein homolog Source.9: DFBPPR10938 ---- Animal proteins ---- Serine/threonine-protein kinase mos Source.10: DFBPPR12068 ---- Animal proteins ---- Serine/arginine repetitive matrix protein 1 Source.11: DFBPPR12734 ---- Animal proteins ---- Sodium-dependent phosphate transport protein 2A Source.12: DFBPPR13753 ---- Animal proteins ---- Sodium-dependent phosphate transport protein 2A
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity

Peptide	DPPH radical scavenging activity (%)
Peptide	0.01 (mM)	0.1 (mM)	1 (mM)	100 (mM)
TPSPR	2.7 ± 0.5	3.8 ± 1.2	5.5 ± 2.2	11.0 ± 5.6

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with alkaline proteinase Alcalase.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANTH0086
[D2]	DFBPALAM0013
[D3]	DFBPALGL0004
[D4]	DFBPMUFU0672

[D5]

[D6]

[D7]

[D8]

Reference(s)

Primary literature	Yu Z, Liu B, Zhao W, Yin Y, Liu J, Chen F. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein. Food Chem. 2012 Jul 15;133(2):315-22. PMID: 25683401 DOI: 10.1016/j.foodchem.2012.01.032
Other literature(s)	N.D
PubDate	2012