Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0645(Antioxidative peptide)

DFBP ID	DFBPANOX0645
Peptide sequence	EFLQ
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Glu-Phe-Leu-Gln

Single-letter amino acid

EFLQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
535.3 Da	535.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.1000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hemp (Cannabis sativa L.)
Precursor protein	Hemp seed protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0843 ---- Plant proteins ---- MADS-box transcription factor 1 Source.2: DFBPPR0847 ---- Plant proteins ---- Strigolactone esterase D14 Source.3: DFBPPR0954 ---- Plant proteins ---- Leucine-rich repeat receptor protein kinase MSP1 Source.4: DFBPPR1032 ---- Plant proteins ---- Disease resistance protein RGA4 Source.5: DFBPPR1184 ---- Plant proteins ---- Lysine-specific demethylase SE14 Source.6: DFBPPR1489 ---- Plant proteins ---- Protein SUBSTANDARD STARCH GRAIN 4, chloroplastic Source.7: DFBPPR1603 ---- Plant proteins ---- MADS-box transcription factor 5 Source.8: DFBPPR1617 ---- Plant proteins ---- DNA replication licensing factor MCM7 Source.9: DFBPPR1955 ---- Plant proteins ---- Endoribonuclease Dicer homolog 3b Source.10: DFBPPR2630 ---- Plant proteins ---- Probable protein phosphatase 2C 34 Source.11: DFBPPR2775 ---- Plant proteins ---- Auxin response factor 17 Source.12: DFBPPR2978 ---- Plant proteins ---- Disease resistance protein RGA4 Source.13: DFBPPR3053 ---- Plant proteins ---- Beta-glucosidase 18 Source.14: DFBPPR3140 ---- Plant proteins ---- ABC transporter G family member 51 Source.15: DFBPPR3317 ---- Plant proteins ---- Beta-glucosidase 13 Source.16: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.17: DFBPPR3790 ---- Plant proteins ---- Pantothenate kinase 1 Source.18: DFBPPR3802 ---- Plant proteins ---- Dehydration-responsive element-binding protein 2E Source.19: DFBPPR3814 ---- Plant proteins ---- Probable RNA-dependent RNA polymerase 2 Source.20: DFBPPR4224 ---- Plant proteins ---- Probable calcium-binding protein CML22 Source.21: DFBPPR4234 ---- Plant proteins ---- 4-coumarate--CoA ligase-like 3 Source.22: DFBPPR4419 ---- Plant proteins ---- Formin-like protein 15 Source.23: DFBPPR4788 ---- Plant proteins ---- Putative B3 domain-containing protein Os03g0621600 Source.24: DFBPPR4992 ---- Plant proteins ---- Glycinin G3 Source.25: DFBPPR5135 ---- Plant proteins ---- Chalcone--flavonone isomerase 1B-2 Source.26: DFBPPR5242 ---- Plant proteins ---- Chalcone--flavonone isomerase 1B-1 Source.27: DFBPPR5255 ---- Plant proteins ---- Chalcone--flavonone isomerase 2-B Source.28: DFBPPR5294 ---- Plant proteins ---- Protein Ycf2 Source.29: DFBPPR5579 ---- Plant proteins ---- Indole-3-acetaldehyde oxidase Source.30: DFBPPR6380 ---- Plant proteins ---- Legumin A Source.31: DFBPPR7128 ---- Plant proteins ---- Methylthioribose-1-phosphate isomerase Source.32: DFBPPR7600 ---- Milk proteins ---- UDP-glucuronosyltransferase 1A1 Source.33: DFBPPR8367 ---- Plant proteins ---- 13S globulin seed storage protein 2 Source.34: DFBPPR8445 ---- Plant proteins ---- Maturase K Source.35: DFBPPR15980 ---- Animal proteins ---- Peroxisome proliferator-activated receptor alpha Source.36: DFBPPR16040 ---- Animal proteins ---- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Source.37: DFBPPR16128 ---- Animal proteins ---- Cubilin Source.38: DFBPPR16320 ---- Animal proteins ---- Guanylate cyclase soluble subunit beta-1 Source.39: DFBPPR16965 ---- Animal proteins ---- Adseverin Source.40: DFBPPR17006 ---- Animal proteins ---- Syntaxin-17 Source.41: DFBPPR17010 ---- Animal proteins ---- Sestrin-2 Source.42: DFBPPR17059 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Source.43: DFBPPR17091 ---- Animal proteins ---- Extracellular calcium-sensing receptor Source.44: DFBPPR17374 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 1 Source.45: DFBPPR17502 ---- Animal proteins ---- V-type proton ATPase subunit B, kidney isoform Source.46: DFBPPR17515 ---- Animal proteins ---- 5'-nucleotidase Source.47: DFBPPR17523 ---- Animal proteins ---- DNA (cytosine-5)-methyltransferase 1 Source.48: DFBPPR17693 ---- Animal proteins ---- Carnitine O-palmitoyltransferase 2, mitochondrial Source.49: DFBPPR17794 ---- Animal proteins ---- Pyruvate carboxylase, mitochondrial Source.50: DFBPPR17914 ---- Animal proteins ---- Glycerol-3-phosphate acyltransferase 1, mitochondrial Source.51: DFBPPR17958 ---- Animal proteins ---- Homeobox protein NANOG Source.52: DFBPPR17975 ---- Animal proteins ---- Peroxisomal N(1)-acetyl-spermine/spermidine oxidase Source.53: DFBPPR18134 ---- Animal proteins ---- Cyclin-T1 Source.54: DFBPPR18198 ---- Animal proteins ---- V-type proton ATPase subunit B, brain isoform Source.55: DFBPPR18260 ---- Animal proteins ---- 2-oxoglutarate dehydrogenase, mitochondrial Source.56: DFBPPR18313 ---- Animal proteins ---- Septin-12 Source.57: DFBPPR18319 ---- Animal proteins ---- MMS19 nucleotide excision repair protein homolog Source.58: DFBPPR18341 ---- Animal proteins ---- BRISC complex subunit Abraxas 2 Source.59: DFBPPR18377 ---- Animal proteins ---- Importin subunit alpha-5 Source.60: DFBPPR18395 ---- Animal proteins ---- Galactosylceramide sulfotransferase Source.61: DFBPPR18548 ---- Animal proteins ---- Lipoyl synthase, mitochondrial Source.62: DFBPPR18570 ---- Animal proteins ---- Prolyl 3-hydroxylase OGFOD1 Source.63: DFBPPR18750 ---- Animal proteins ---- Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase Source.64: DFBPPR18957 ---- Animal proteins ---- Glycerol-3-phosphate dehydrogenase, mitochondrial Source.65: DFBPPR18983 ---- Animal proteins ---- Endothelial protein C receptor Source.66: DFBPPR19239 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 42 Source.67: DFBPPR19322 ---- Animal proteins ---- Interferon alpha-A Source.68: DFBPPR19336 ---- Animal proteins ---- Arf-GAP domain and FG repeat-containing protein 1 Source.69: DFBPPR19565 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 4 Source.70: DFBPPR19597 ---- Animal proteins ---- Protein HEXIM1 Source.71: DFBPPR19735 ---- Animal proteins ---- Complement component C7 Source.72: DFBPPR19866 ---- Animal proteins ---- Actin-related protein 8 Source.73: DFBPPR20025 ---- Animal proteins ---- Importin subunit alpha-7 Source.74: DFBPPR20293 ---- Animal proteins ---- Cytosolic arginine sensor for mTORC1 subunit 1 Source.75: DFBPPR20346 ---- Animal proteins ---- Serpin B6 Source.76: DFBPPR20362 ---- Animal proteins ---- [Protein ADP-ribosylarginine] hydrolase Source.77: DFBPPR20540 ---- Animal proteins ---- Structure-specific endonuclease subunit SLX1 Source.78: DFBPPR20581 ---- Animal proteins ---- Membrane magnesium transporter 1 Source.79: DFBPPR20673 ---- Animal proteins ---- Trafficking protein particle complex subunit 9 Source.80: DFBPPR20675 ---- Animal proteins ---- MYG1 exonuclease Source.81: DFBPPR20967 ---- Animal proteins ---- Phosducin-like protein Source.82: DFBPPR21088 ---- Animal proteins ---- Centrosomal protein 43 Source.83: DFBPPR21122 ---- Animal proteins ---- Derlin-3 Source.84: DFBPPR21154 ---- Animal proteins ---- Proton-activated chloride channel Source.85: DFBPPR21330 ---- Animal proteins ---- 60S ribosomal export protein NMD3 Source.86: DFBPPR21340 ---- Animal proteins ---- Ribosome-releasing factor 2, mitochondrial Source.87: DFBPPR21361 ---- Animal proteins ---- Seizure protein 6 homolog Source.88: DFBPPR21506 ---- Animal proteins ---- Origin recognition complex subunit 3 Source.89: DFBPPR21879 ---- Animal proteins ---- Protein SDA1 homolog Source.90: DFBPPR22027 ---- Animal proteins ---- F-box/LRR-repeat protein 4 Source.91: DFBPPR8530 ---- Animal proteins ---- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Source.92: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.93: DFBPPR8578 ---- Animal proteins ---- Large proline-rich protein BAG6 Source.94: DFBPPR8908 ---- Animal proteins ---- Extracellular calcium-sensing receptor Source.95: DFBPPR8923 ---- Animal proteins ---- Cubilin Source.96: DFBPPR9306 ---- Animal proteins ---- Complement component C7 Source.97: DFBPPR10177 ---- Animal proteins ---- Talin-1 Source.98: DFBPPR10199 ---- Animal proteins ---- Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Source.99: DFBPPR10289 ---- Animal proteins ---- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Source.100: DFBPPR10308 ---- Animal proteins ---- DNA (cytosine-5)-methyltransferase 1 Source.101: DFBPPR10329 ---- Animal proteins ---- Activity-regulated cytoskeleton-associated protein Source.102: DFBPPR10452 ---- Animal proteins ---- Desmin Source.103: DFBPPR10497 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 7 Source.104: DFBPPR10637 ---- Animal proteins ---- CTD small phosphatase-like protein Source.105: DFBPPR10735 ---- Animal proteins ---- Semaphorin-3E Source.106: DFBPPR11086 ---- Animal proteins ---- Zinc finger E-box-binding homeobox 1 Source.107: DFBPPR11104 ---- Animal proteins ---- Importin subunit alpha-5 Source.108: DFBPPR11124 ---- Animal proteins ---- Phosphatidylinositol 4-phosphate 5-kinase type-1 beta Source.109: DFBPPR11168 ---- Animal proteins ---- Trimethyllysine dioxygenase, mitochondrial Source.110: DFBPPR11273 ---- Animal proteins ---- Carbohydrate sulfotransferase 3 Source.111: DFBPPR11374 ---- Animal proteins ---- Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 Source.112: DFBPPR11507 ---- Animal proteins ---- Outer dense fiber protein 2 Source.113: DFBPPR11593 ---- Animal proteins ---- Syndetin Source.114: DFBPPR11727 ---- Animal proteins ---- Peroxisomal targeting signal 1 receptor Source.115: DFBPPR11787 ---- Animal proteins ---- V-type proton ATPase subunit B Source.116: DFBPPR11817 ---- Animal proteins ---- Meiosis regulator and mRNA stability factor 1 Source.117: DFBPPR11833 ---- Animal proteins ---- Kelch-like protein 7 Source.118: DFBPPR12176 ---- Animal proteins ---- Serine/threonine-protein kinase 11-interacting protein Source.119: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.120: DFBPPR12374 ---- Animal proteins ---- Sortilin-related receptor Source.121: DFBPPR12427 ---- Animal proteins ---- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 Source.122: DFBPPR12463 ---- Animal proteins ---- Interleukin-15 Source.123: DFBPPR12765 ---- Animal proteins ---- Chloride transport protein 6 Source.124: DFBPPR13298 ---- Animal proteins ---- Cyclin-T1 Source.125: DFBPPR13641 ---- Animal proteins ---- Interferon alpha/beta receptor 1 Source.126: DFBPPR14167 ---- Marine protein ---- Actin-related protein 8 Source.127: DFBPPR14406 ---- Marine protein ---- ATP synthase subunit a, chloroplastic Source.128: DFBPPR14918 ---- Microorganism protein ---- Isocitrate lyase Source.129: DFBPPR15155 ---- Microorganism protein ---- Crossover junction endonuclease MUS81 Source.130: DFBPPR15209 ---- Microorganism protein ---- Chromatin modification-related protein EAF3 Source.131: DFBPPR15215 ---- Microorganism protein ---- Protein transport protein SEC24 Source.132: DFBPPR15409 ---- Microorganism protein ---- Mitochondrial inner membrane magnesium transporter MRS2 Source.133: DFBPPR15471 ---- Microorganism protein ---- Polynucleotide 5'-hydroxyl-kinase GRC3 Source.134: DFBPPR15557 ---- Microorganism protein ---- DNA damage checkpoint protein LCD1 Source.135: DFBPPR7932 ---- Plant protein ---- Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic Source.136: DFBPPR7937 ---- Plant protein ---- Alpha-glucan phosphorylase, H isozyme Source.137: DFBPPR7973 ---- Plant protein ---- Maturase K Source.138: DFBPPR8072 ---- Plant protein ---- Phosphoenolpyruvate carboxylase Source.139: DFBPPR8155 ---- Plant protein ---- Protein Ycf2 Source.140: DFBPPR8300 ---- Plant protein ---- Protein Ycf2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	At 0.5 mg/mL, EFLQ was an active antioxidant peptide with 15% DPPH scavenging and metal chelation activity of 89%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hemp seed proteins was hydrolyzed by simulating gastrointestinal tract digestion (Pepsin and Pancreatin).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide showed 64% renin inhibition.

Database cross-references

DFBP

[D1]	DFBPACEI1288
[D2]	DFBPMUFU0295

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Girgih, A.T., He, R., Malomo, S., Offengenden, M., Wu, J., Aluko, R.E. Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides. Journal of Functional Foods. 2014, 6, 384-94. DOI: 10.1016/j.jff.2013.11.005
Other literature(s)	N.D
PubDate	2014