Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0665(Antioxidative peptide)

DFBP ID	DFBPANOX0665
Peptide sequence	EFIQ
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Glu-Phe-Ile-Gln

Single-letter amino acid

EFIQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
535.3 Da	535.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.0750^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hemp (Cannabis sativa L.)
Precursor protein	Hemp seed protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0853 ---- Plant proteins ---- Protein ETHYLENE-INSENSITIVE 3-like 1a Source.2: DFBPPR1591 ---- Plant proteins ---- Protein ETHYLENE-INSENSITIVE 3-like 1b Source.3: DFBPPR2301 ---- Plant proteins ---- Red chlorophyll catabolite reductase 1, chloroplastic Source.4: DFBPPR2671 ---- Plant proteins ---- Proteasome subunit beta type-2 Source.5: DFBPPR3008 ---- Plant proteins ---- Potassium channel KOR1 Source.6: DFBPPR3241 ---- Plant proteins ---- Elongation factor 1-delta 2 Source.7: DFBPPR4097 ---- Plant proteins ---- Trafficking protein particle complex II-specific subunit 130 homolog Source.8: DFBPPR4772 ---- Plant proteins ---- SEC1 family transport protein SLY1 Source.9: DFBPPR4869 ---- Plant proteins ---- Putative B3 domain-containing protein LOC_Os07g12820 Source.10: DFBPPR7070 ---- Plant proteins ---- Red chlorophyll catabolite reductase Source.11: DFBPPR8363 ---- Plant proteins ---- 13S globulin seed storage protein 1 Source.12: DFBPPR8365 ---- Plant proteins ---- 13S globulin seed storage protein 3 Source.13: DFBPPR17300 ---- Animal proteins ---- Oxidized low-density lipoprotein receptor 1 Source.14: DFBPPR17301 ---- Animal proteins ---- N-fatty-acyl-amino acid synthase/hydrolase PM20D1 Source.15: DFBPPR17668 ---- Animal proteins ---- 2'-5'-oligoadenylate synthase 2 Source.16: DFBPPR18562 ---- Animal proteins ---- Neuronal calcium sensor 1 Source.17: DFBPPR18716 ---- Animal proteins ---- 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial Source.18: DFBPPR18775 ---- Animal proteins ---- tRNA methyltransferase 10 homolog A Source.19: DFBPPR19543 ---- Animal proteins ---- Protein transport protein Sec23A Source.20: DFBPPR19560 ---- Animal proteins ---- Protein transport protein Sec23B Source.21: DFBPPR19612 ---- Animal proteins ---- Lysosomal-trafficking regulator Source.22: DFBPPR20380 ---- Animal proteins ---- Signal recognition particle receptor subunit alpha Source.23: DFBPPR20617 ---- Animal proteins ---- GTPase-activating protein and VPS9 domain-containing protein 1 Source.24: DFBPPR20848 ---- Animal proteins ---- Protein VAC14 homolog Source.25: DFBPPR20949 ---- Animal proteins ---- Synaptogyrin-2 Source.26: DFBPPR21281 ---- Animal proteins ---- 28S ribosomal protein S36, mitochondrial Source.27: DFBPPR21319 ---- Animal proteins ---- V-type proton ATPase subunit H Source.28: DFBPPR22319 ---- Animal proteins ---- Regulator of G-protein signaling 5 Source.29: DFBPPR22353 ---- Animal proteins ---- Calmodulin-like protein 4 Source.30: DFBPPR22615 ---- Animal proteins ---- Coiled-coil domain-containing protein 54 Source.31: DFBPPR8629 ---- Animal proteins ---- 2'-5'-oligoadenylate synthase 1 Source.32: DFBPPR8829 ---- Animal proteins ---- Oxidized low-density lipoprotein receptor 1 Source.33: DFBPPR9806 ---- Animal proteins ---- V-type proton ATPase subunit H Source.34: DFBPPR9932 ---- Animal proteins ---- Regulator of G-protein signaling 5 Source.35: DFBPPR10459 ---- Animal proteins ---- Reelin Source.36: DFBPPR10491 ---- Animal proteins ---- Neuronal calcium sensor 1 Source.37: DFBPPR11028 ---- Animal proteins ---- Interleukin-6 Source.38: DFBPPR11236 ---- Animal proteins ---- Protein transport protein Sec23A Source.39: DFBPPR11880 ---- Animal proteins ---- Deleted in azoospermia-like Source.40: DFBPPR11917 ---- Animal proteins ---- Protein VAC14 homolog Source.41: DFBPPR13187 ---- Animal proteins ---- Toll-like receptor 4 Source.42: DFBPPR14304 ---- Marine protein ---- ATP synthase subunit a, chloroplastic Source.43: DFBPPR15371 ---- Microorganism protein ---- Protein HIR2 Source.44: DFBPPR15648 ---- Microorganism protein ---- Chromosome segregation in meiosis protein 2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	At 0.5 mg/mL, EFIQ was an active antioxidant peptide with 25% DPPH scavenging and metal chelation activity of 81%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hemp seed proteins was hydrolyzed by simulating gastrointestinal tract digestion (Pepsin and Pancreatin).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide showed 36% renin inhibition.

Database cross-references

DFBP

[D1]	DFBPACEI1300
[D2]	DFBPMUFU0307

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Girgih, A.T., He, R., Malomo, S., Offengenden, M., Wu, J., Aluko, R.E. Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides. Journal of Functional Foods. 2014, 6, 384-94. DOI: 10.1016/j.jff.2013.11.005
Other literature(s)	N.D
PubDate	2014