Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0710(Antioxidative peptide)

DFBP ID	DFBPANOX0710
Peptide sequence	HLPLP
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

His-Leu-Pro-Leu-Pro

Single-letter amino acid

HLPLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
575.3 Da	575.70 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.2400^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(125-129)
Precursor protein(s) search	Source.1: DFBPPR1798 ---- Plant proteins ---- UMP-CMP kinase 2 Source.2: DFBPPR3187 ---- Plant proteins ---- Probable glucuronosyltransferase Os10g0205300 Source.3: DFBPPR4827 ---- Plant proteins ---- BURP domain-containing protein 1 Source.4: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.5: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.6: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.7: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.8: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.9: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.10: DFBPPR16229 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1A Source.11: DFBPPR16270 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.12: DFBPPR17683 ---- Animal proteins ---- Cyanocobalamin reductase / alkylcobalamin dealkylase Source.13: DFBPPR19085 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.14: DFBPPR21483 ---- Animal proteins ---- F-box/LRR-repeat protein 8 Source.15: DFBPPR13225 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1A Source.16: DFBPPR15017 ---- Microorganism protein ---- Serine/threonine-protein kinase TEL1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide showed almost no ABTS^●+ radical scavenging activity (TEAC value of < 0.1 μmol Trolox equiv μmol^-1 of peptide).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Peptide obtained by hydrolysis of human milk by use of Pepsin and Pancreatin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0419, DFBPACEI0418
[D2]	DFBPANHY0003
[D3]	DFBPMUFU0116

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Hernández-Ledesma, B., Quirós, A., Amigo, L., Recio, I. Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. International Dairy Journal. 2007, 17, 42-9. DOI: 10.1016/j.idairyj.2005.12.012
Other literature(s)	N.D
PubDate	2007