Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0718(Antioxidative peptide)

DFBP ID	DFBPANOX0718
Peptide sequence	EIPT
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antimicrobial activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Glu-Ile-Pro-Thr

Single-letter amino acid

EIPT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	458.50 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.3250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	κ-Casein
Residue position	f(118-121)
Precursor protein(s) search	Source.1: DFBPPR0929 ---- Plant proteins ---- Protein ROS1A Source.2: DFBPPR1021 ---- Plant proteins ---- L-ascorbate peroxidase 1, cytosolic Source.3: DFBPPR1354 ---- Plant proteins ---- Brassinosteroid LRR receptor kinase BRL3 Source.4: DFBPPR2373 ---- Plant proteins ---- Adagio-like protein 3 Source.5: DFBPPR2402 ---- Plant proteins ---- Tyrosine decarboxylase Source.6: DFBPPR2670 ---- Plant proteins ---- Putative germin-like protein 2-2 Source.7: DFBPPR3055 ---- Plant proteins ---- Chitin-inducible gibberellin-responsive protein 1 Source.8: DFBPPR3779 ---- Plant proteins ---- Probable nucleoredoxin 2 Source.9: DFBPPR4130 ---- Plant proteins ---- Two-component response regulator-like PRR95 Source.10: DFBPPR5013 ---- Plant proteins ---- Leucine-rich repeat receptor-like kinase protein CLV1a Source.11: DFBPPR5032 ---- Plant proteins ---- NAD(P)H-dependent 6'-deoxychalcone synthase Source.12: DFBPPR5340 ---- Plant proteins ---- 30S ribosomal protein S2, chloroplastic Source.13: DFBPPR6027 ---- Plant proteins ---- Phosphoenolpyruvate carboxykinase (ATP) Source.14: DFBPPR6396 ---- Plant proteins ---- Hydroxyproline O-arabinosyltransferase NOD3 Source.15: DFBPPR7519 ---- Plant proteins ---- Chaperonin CPN60, mitochondrial Source.16: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.17: DFBPPR8492 ---- Milk proteins ---- Kappa-casein Source.18: DFBPPR16263 ---- Animal proteins ---- Protein 4.1 Source.19: DFBPPR16685 ---- Animal proteins ---- Bromodomain-containing protein 2 Source.20: DFBPPR16748 ---- Animal proteins ---- Thyroid adenoma-associated protein homolog Source.21: DFBPPR17893 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 3 Source.22: DFBPPR17916 ---- Animal proteins ---- E3 ubiquitin-protein ligase RNF126 Source.23: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.24: DFBPPR18605 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.25: DFBPPR18967 ---- Animal proteins ---- Krev interaction trapped protein 1 Source.26: DFBPPR20042 ---- Animal proteins ---- Neuroendocrine convertase 1 Source.27: DFBPPR20550 ---- Animal proteins ---- G-protein coupled receptor family C group 6 member A Source.28: DFBPPR20668 ---- Animal proteins ---- BLOC-1-related complex subunit 5 Source.29: DFBPPR20999 ---- Animal proteins ---- Protein SERAC1 Source.30: DFBPPR21101 ---- Animal proteins ---- Bromodomain-containing protein 2 Source.31: DFBPPR21506 ---- Animal proteins ---- Origin recognition complex subunit 3 Source.32: DFBPPR21533 ---- Animal proteins ---- Zinc finger protein 19 Source.33: DFBPPR21553 ---- Animal proteins ---- Transmembrane protein 135 Source.34: DFBPPR21767 ---- Animal proteins ---- Tudor domain-containing protein 5 Source.35: DFBPPR8902 ---- Animal proteins ---- Cytochrome P450 2E1 Source.36: DFBPPR9564 ---- Animal proteins ---- Inter-alpha-trypsin inhibitor heavy chain H2 Source.37: DFBPPR9746 ---- Animal proteins ---- Valine--tRNA ligase, mitochondrial Source.38: DFBPPR10458 ---- Animal proteins ---- Neurofascin Source.39: DFBPPR10888 ---- Animal proteins ---- Nuclear distribution protein nudE-like 1 Source.40: DFBPPR11044 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.41: DFBPPR11242 ---- Animal proteins ---- GDNF family receptor alpha-1 Source.42: DFBPPR11567 ---- Animal proteins ---- Ovocalyxin-32 Source.43: DFBPPR11934 ---- Animal proteins ---- Gametogenetin-binding protein 2 Source.44: DFBPPR11978 ---- Animal proteins ---- ER membrane protein complex subunit 1 Source.45: DFBPPR12036 ---- Animal proteins ---- BLOC-1-related complex subunit 5 Source.46: DFBPPR12176 ---- Animal proteins ---- Serine/threonine-protein kinase 11-interacting protein Source.47: DFBPPR15017 ---- Microorganism protein ---- Serine/threonine-protein kinase TEL1 Source.48: DFBPPR15409 ---- Microorganism protein ---- Mitochondrial inner membrane magnesium transporter MRS2 Source.49: DFBPPR15446 ---- Microorganism protein ---- Pre-rRNA-processing protein RIX1 Source.50: DFBPPR15712 ---- Microorganism protein ---- Survival factor 1 Source.51: DFBPPR8034 ---- Plant protein ---- Linoleate 9S-lipoxygenase 1 Source.52: DFBPPR8162 ---- Plant protein ---- 30S ribosomal protein S2, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide with the sequence EIPT didn't showed oxygen radical-scavenging activity (ORAC-FL value of 0 μmol Trolox* equivalents per μmol of peptide). *Trolox - 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, a water-soluble derivative of vitamin E.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Peptide obtained by hydrolysis of bovine κ-casein by use of porcine pepsin A.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1722
[D2]	DFBPAMIC0493
[D3]	DFBPMUFU0499

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	López-Expósito, I., Quirós, A., Amigo, L., Recio, I. Casein hydrolysates as a source of antimicrobial, antioxidant and antihypertensive peptides. Le Lait. 2007, 87, 241-9. DOI: 10.1051/lait:2007019
Other literature(s)	N.D
PubDate	2007