Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0720(Antioxidative peptide)

DFBP ID	DFBPANOX0720
Peptide sequence	RINK
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Arg-Ile-Asn-Lys

Single-letter amino acid

RINK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	529.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.54 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.8500^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	β-Casein
Residue position	f(25-28)
Precursor protein(s) search	Source.1: DFBPPR0947 ---- Plant proteins ---- Histone-lysine N-methyltransferase TRX1 Source.2: DFBPPR1406 ---- Plant proteins ---- Acetyl-CoA carboxylase 1 Source.3: DFBPPR2221 ---- Plant proteins ---- L-galactono-1,4-lactone dehydrogenase 2, mitochondrial Source.4: DFBPPR2296 ---- Plant proteins ---- L-galactono-1,4-lactone dehydrogenase 1, mitochondrial Source.5: DFBPPR2524 ---- Plant proteins ---- Serine carboxypeptidase 3 Source.6: DFBPPR2863 ---- Plant proteins ---- Serine carboxypeptidase-like Source.7: DFBPPR3067 ---- Plant proteins ---- 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 Source.8: DFBPPR3202 ---- Plant proteins ---- Probable 4-coumarate--CoA ligase 3 Source.9: DFBPPR3876 ---- Plant proteins ---- Bifunctional nuclease 2 Source.10: DFBPPR3912 ---- Plant proteins ---- Sialyltransferase-like protein 4 Source.11: DFBPPR3968 ---- Plant proteins ---- CRS2-associated factor 1, chloroplastic Source.12: DFBPPR4331 ---- Plant proteins ---- 60S ribosomal protein L10-2 Source.13: DFBPPR4504 ---- Plant proteins ---- 60S ribosomal protein L10-1 Source.14: DFBPPR5907 ---- Plant proteins ---- Probable DNA-directed RNA polymerase Source.15: DFBPPR6103 ---- Plant proteins ---- 60S ribosomal protein L10 Source.16: DFBPPR6759 ---- Plant proteins ---- Serine carboxypeptidase 3 Source.17: DFBPPR7010 ---- Plant proteins ---- Serine carboxypeptidase 3 Source.18: DFBPPR7111 ---- Plant proteins ---- Methionine S-methyltransferase Source.19: DFBPPR7640 ---- Milk proteins ---- Pro-neuregulin-1, membrane-bound isoform Source.20: DFBPPR8430 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.21: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.22: DFBPPR8502 ---- Milk proteins ---- Broad substrate specificity ATP-binding cassette transporter ABCG2 Source.23: DFBPPR15958 ---- Animal proteins ---- Myosin-9 Source.24: DFBPPR16563 ---- Animal proteins ---- Myosin-16 Source.25: DFBPPR16567 ---- Animal proteins ---- Oxygen-regulated protein 1 Source.26: DFBPPR16847 ---- Animal proteins ---- Fibrinogen alpha chain Source.27: DFBPPR17123 ---- Animal proteins ---- Retinal guanylyl cyclase 2 Source.28: DFBPPR17282 ---- Animal proteins ---- Myosin-10 Source.29: DFBPPR17294 ---- Animal proteins ---- Ezrin Source.30: DFBPPR17341 ---- Animal proteins ---- Radixin Source.31: DFBPPR17401 ---- Animal proteins ---- Moesin Source.32: DFBPPR17462 ---- Animal proteins ---- DNA replication ATP-dependent helicase/nuclease DNA2 Source.33: DFBPPR18414 ---- Animal proteins ---- NADPH--cytochrome P450 reductase Source.34: DFBPPR18636 ---- Animal proteins ---- AP-2 complex subunit alpha-2 Source.35: DFBPPR18763 ---- Animal proteins ---- UTP--glucose-1-phosphate uridylyltransferase Source.36: DFBPPR18792 ---- Animal proteins ---- Integral membrane protein 2C Source.37: DFBPPR18962 ---- Animal proteins ---- Sodium/potassium-transporting ATPase subunit beta-2 Source.38: DFBPPR18997 ---- Animal proteins ---- Striatin-3 Source.39: DFBPPR19183 ---- Animal proteins ---- Testis anion transporter 1 Source.40: DFBPPR19958 ---- Animal proteins ---- 60S ribosomal protein L10 Source.41: DFBPPR20055 ---- Animal proteins ---- Myelin protein zero-like protein 1 Source.42: DFBPPR20572 ---- Animal proteins ---- Leucine-rich repeat protein SHOC-2 Source.43: DFBPPR21659 ---- Animal proteins ---- Peptide chain release factor 1-like, mitochondrial Source.44: DFBPPR21695 ---- Animal proteins ---- Choline transporter-like protein 3 Source.45: DFBPPR22065 ---- Animal proteins ---- 60S ribosomal protein L10-like Source.46: DFBPPR8711 ---- Animal proteins ---- Fumarate hydratase, mitochondrial Source.47: DFBPPR9233 ---- Animal proteins ---- Integral membrane protein 2C Source.48: DFBPPR9236 ---- Animal proteins ---- Radixin Source.49: DFBPPR9294 ---- Animal proteins ---- 60S ribosomal protein L10 Source.50: DFBPPR9363 ---- Animal proteins ---- Moesin Source.51: DFBPPR9425 ---- Animal proteins ---- UTP--glucose-1-phosphate uridylyltransferase Source.52: DFBPPR10083 ---- Animal proteins ---- Myosin-9 Source.53: DFBPPR10261 ---- Animal proteins ---- Cadherin-13 Source.54: DFBPPR11390 ---- Animal proteins ---- Tuftelin-interacting protein 11 Source.55: DFBPPR11655 ---- Animal proteins ---- 60S ribosomal protein L10 Source.56: DFBPPR11770 ---- Animal proteins ---- Protein fem-1 homolog B Source.57: DFBPPR11786 ---- Animal proteins ---- Leucine-rich repeat protein SHOC-2 Source.58: DFBPPR11864 ---- Animal proteins ---- Radixin Source.59: DFBPPR12316 ---- Animal proteins ---- Sodium/potassium-transporting ATPase subunit beta-2 Source.60: DFBPPR12500 ---- Animal proteins ---- Cystathionine beta-synthase Source.61: DFBPPR12501 ---- Animal proteins ---- Ezrin Source.62: DFBPPR13843 ---- Animal proteins ---- 60S ribosomal protein L10 Source.63: DFBPPR14151 ---- Marine protein ---- Peptidyl-tRNA hydrolase ICT1, mitochondrial Source.64: DFBPPR14312 ---- Marine protein ---- Cytochrome c biogenesis protein CcsA Source.65: DFBPPR14882 ---- Microorganism protein ---- ATP-dependent 6-phosphofructokinase subunit beta Source.66: DFBPPR15134 ---- Microorganism protein ---- Eukaryotic translation initiation factor 3 subunit A Source.67: DFBPPR15196 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP8 Source.68: DFBPPR15353 ---- Microorganism protein ---- Pre-mRNA-splicing factor ISY1 Source.69: DFBPPR15453 ---- Microorganism protein ---- ATP synthase subunit 5, mitochondrial Source.70: DFBPPR15527 ---- Microorganism protein ---- Biogenesis of lysosome-related organelles complex 1 subunit SNN1 Source.71: DFBPPR15876 ---- Microorganism protein ---- Probable DNA-directed RNA polymerase Source.72: DFBPPR8239 ---- Plant protein ---- Serine--tRNA ligase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide with the sequence RINK didn't showed oxygen radical-scavenging activity (ORAC-FL value of 0 μmol Trolox* equivalents per μmol of peptide). *Trolox - 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, a water-soluble derivative of vitamin E.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Peptide obtained by hydrolysis of bovine β-casein by use of porcine pepsin A.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1725
[D2]	DFBPMUFU0501

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	López-Expósito, I., Quirós, A., Amigo, L., Recio, I. Casein hydrolysates as a source of antimicrobial, antioxidant and antihypertensive peptides. Le Lait. 2007, 87, 241-9. DOI: 10.1051/lait:2007019
Other literature(s)	N.D
PubDate	2007