The egg peptide LKP possessed potent oxygen radical-scavenging effect, with an ORAC value of 1.27 ± 0.09 μmol TE/μmol, as shown in Table 1. In addition, the egg peptides IRW and LKP showed synergistic effects with the phytochemicals vitamin C, EGCG, and caffeic acid at high molar ratios. Table 1. The oxygen radical absorbance capacity values (trolox equivalent) of the whole protein/hydrolysate/peptides/amino acids of ovotransferrin and four phytochemicals.
Sample
| ORAC valuea
| μmol TE/μmol
| μmol TE/mg
| Whole protein (ovotransferrin)
|
| 0.21 ± 0.03
| Hydrolysate (thermolysin-pepsin)
|
| 1.69 ± 0.15c
| IRW
| 6.63 ± 0.37d
| 14.0 ± 0.78
| IR | 0.29 ± 0.02d
| 1.00 ± 0.06d
| RW
| 10.1 ± 0.54d
| 28.3 ± 1.51d
| I
| 0.54 ± 0.04d,e
| 4.11 ± 0.30d
| R
| 0.57 ± 0.03e
| 3.25 ± 0.15d,e
| W
| 7.22 ± 0.84
| 35.4 ± 4.11e
| LKP
| 1.27 ± 0.09
| 3.55 ± 0.25
| LK
| 0.34 ± 0.05
| 1.30 ± 0.18
| KP
| 0.29 ± 0.04
| 1.19 ± 0.17
| L
| 0.89 ± 0.01
| 6.78 ± 0.06
| K
| 1.03 ± 0.08
| 7.03 ± 0.55
| P
| 0.65 ± 0.03
| 5.68 ± 0.26
| Vitamin C
| 1.65 ± 0.11
| 9.35 ± 0.63
| EGCG
| 6.42 ± 0.46
| 14.0 ± 1.00
| Caffeic acid
| 10.4 ± 0.43
| 57.5 ± 2.39
| Quercetin
| 11.9 ± 0.74
| 39.4 ± 2.45
| BHT (positive control)
| 5.30 ± 0.26
| 24.1 ± 1.18
| LSD (p < 0.05)b
| 0.605
| 2.301
| a All the data were expressed as mean ± standard deviation (SD). b LSD (p < 0.05), least significant difference, was used for comparison between means of various samples. c Indicated p < 0.001 compared to the whole protein by t-test. d Indicated p < 0.001 compared to IRW by Tukey's test. e Indicated p < 0.001 compared to RW by Tukey's test.
| |