Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0956(Antioxidative peptide)

DFBP ID	DFBPANOX0956
Peptide sequence	PMH
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Met-His

Single-letter amino acid

PMH

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	383.47 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9667^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine whey protein
Precursor protein	β-Lactoglobulin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0115 ---- Milk proteins ---- Beta-lactoglobulin Source.2: DFBPPR0860 ---- Plant proteins ---- Soluble starch synthase 3a, chloroplastic/amyloplastic Source.3: DFBPPR0917 ---- Plant proteins ---- Ethylene receptor 2 Source.4: DFBPPR1013 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase 1B Source.5: DFBPPR1074 ---- Plant proteins ---- Protein DWARF AND LOW-TILLERING Source.6: DFBPPR1264 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase 1A Source.7: DFBPPR1362 ---- Plant proteins ---- Protein ROS1C Source.8: DFBPPR1528 ---- Plant proteins ---- Cyclin-dependent kinase C-2 Source.9: DFBPPR1534 ---- Plant proteins ---- Beta-fructofuranosidase, insoluble isoenzyme 5 Source.10: DFBPPR1553 ---- Plant proteins ---- Transcription factor LG2 Source.11: DFBPPR1583 ---- Plant proteins ---- Cytochrome c oxidase subunit 1 Source.12: DFBPPR1612 ---- Plant proteins ---- 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 Source.13: DFBPPR1707 ---- Plant proteins ---- Probable histidine kinase 2 Source.14: DFBPPR1806 ---- Plant proteins ---- Laccase-19 Source.15: DFBPPR1835 ---- Plant proteins ---- Laccase-15 Source.16: DFBPPR1839 ---- Plant proteins ---- Laccase-24 Source.17: DFBPPR1861 ---- Plant proteins ---- Laccase-25 Source.18: DFBPPR1867 ---- Plant proteins ---- Laccase-21 Source.19: DFBPPR1870 ---- Plant proteins ---- Laccase-20 Source.20: DFBPPR1884 ---- Plant proteins ---- Putative laccase-1 Source.21: DFBPPR1889 ---- Plant proteins ---- Laccase-18 Source.22: DFBPPR1922 ---- Plant proteins ---- Cyclin-dependent kinase G-2 Source.23: DFBPPR1988 ---- Plant proteins ---- Protein FLORAL ORGAN NUMBER2 Source.24: DFBPPR2013 ---- Plant proteins ---- Laccase-6 Source.25: DFBPPR2036 ---- Plant proteins ---- Putative laccase-16 Source.26: DFBPPR2082 ---- Plant proteins ---- Putative laccase-9 Source.27: DFBPPR2135 ---- Plant proteins ---- Putative DNA ligase 4 Source.28: DFBPPR2291 ---- Plant proteins ---- UDP-glucose 6-dehydrogenase 3 Source.29: DFBPPR2298 ---- Plant proteins ---- UDP-glucose 6-dehydrogenase 4 Source.30: DFBPPR2312 ---- Plant proteins ---- Probable GTP diphosphokinase RSH3, chloroplastic Source.31: DFBPPR2373 ---- Plant proteins ---- Adagio-like protein 3 Source.32: DFBPPR2385 ---- Plant proteins ---- Cyclin-A1-1 Source.33: DFBPPR2459 ---- Plant proteins ---- Auxin response factor 25 Source.34: DFBPPR2472 ---- Plant proteins ---- Heat stress transcription factor B-4d Source.35: DFBPPR2615 ---- Plant proteins ---- Cytochrome P450 734A5 Source.36: DFBPPR2855 ---- Plant proteins ---- Transcription factor TGAL9 Source.37: DFBPPR2945 ---- Plant proteins ---- Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic Source.38: DFBPPR3009 ---- Plant proteins ---- Probable xyloglucan glycosyltransferase 2 Source.39: DFBPPR3223 ---- Plant proteins ---- Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic Source.40: DFBPPR3229 ---- Plant proteins ---- Transcription factor TGAL7 Source.41: DFBPPR3230 ---- Plant proteins ---- Probable protein phosphatase 2C 37 Source.42: DFBPPR3389 ---- Plant proteins ---- Beta-galactosidase 7 Source.43: DFBPPR3637 ---- Plant proteins ---- Zinc-finger homeodomain protein 4 Source.44: DFBPPR3646 ---- Plant proteins ---- Cyclin-A1-3 Source.45: DFBPPR3673 ---- Plant proteins ---- Zinc-finger homeodomain protein 3 Source.46: DFBPPR3715 ---- Plant proteins ---- Auxin transporter-like protein 3 Source.47: DFBPPR3771 ---- Plant proteins ---- 24-methylenesterol C-methyltransferase 2 Source.48: DFBPPR3932 ---- Plant proteins ---- Cyclin-A1-2 Source.49: DFBPPR3942 ---- Plant proteins ---- Lysine-specific histone demethylase 1 homolog 3 Source.50: DFBPPR4105 ---- Plant proteins ---- Squamosa promoter-binding-like protein 15 Source.51: DFBPPR4460 ---- Plant proteins ---- Protein argonaute 3 Source.52: DFBPPR4517 ---- Plant proteins ---- Protein MEI2-like 3 Source.53: DFBPPR4556 ---- Plant proteins ---- Probable V-type proton ATPase subunit H Source.54: DFBPPR4585 ---- Plant proteins ---- Protein MEI2-like 4 Source.55: DFBPPR4595 ---- Plant proteins ---- Tubby-like F-box protein 9 Source.56: DFBPPR4620 ---- Plant proteins ---- Protein LOL1 Source.57: DFBPPR4692 ---- Plant proteins ---- Probable protein ABIL4 Source.58: DFBPPR4819 ---- Plant proteins ---- B3 domain-containing protein Os08g0324300 Source.59: DFBPPR4895 ---- Plant proteins ---- Carotenoid cleavage dioxygenase 7, chloroplastic Source.60: DFBPPR4902 ---- Plant proteins ---- Endoribonuclease Dicer homolog 1 Source.61: DFBPPR5019 ---- Plant proteins ---- Cytochrome c oxidase subunit 1 Source.62: DFBPPR5041 ---- Plant proteins ---- Diacylglycerol O-acyltransferase 2D Source.63: DFBPPR5580 ---- Plant proteins ---- Cis-zeatin O-glucosyltransferase 1 Source.64: DFBPPR5582 ---- Plant proteins ---- Cytochrome c oxidase subunit 1 Source.65: DFBPPR5749 ---- Plant proteins ---- Cis-zeatin O-glucosyltransferase 2 Source.66: DFBPPR6296 ---- Plant proteins ---- Cytochrome c oxidase subunit 1 Source.67: DFBPPR6471 ---- Plant proteins ---- Beta-amyrin synthase Source.68: DFBPPR6676 ---- Plant proteins ---- Cytochrome c oxidase subunit 1 Source.69: DFBPPR7469 ---- Plant proteins ---- Germin-like protein 1 Source.70: DFBPPR7687 ---- Milk proteins ---- Beta-lactoglobulin Source.71: DFBPPR7698 ---- Milk proteins ---- Beta-lactoglobulin-1/B Source.72: DFBPPR7714 ---- Milk proteins ---- Beta-lactoglobulin Source.73: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin Source.74: DFBPPR15991 ---- Animal proteins ---- Toll-like receptor 9 Source.75: DFBPPR16173 ---- Animal proteins ---- Aromatase Source.76: DFBPPR16252 ---- Animal proteins ---- Steroid hormone receptor ERR1 Source.77: DFBPPR16280 ---- Animal proteins ---- Vasopressin V2 receptor Source.78: DFBPPR16321 ---- Animal proteins ---- Aggrecan core protein Source.79: DFBPPR16656 ---- Animal proteins ---- 60S ribosomal protein L4 Source.80: DFBPPR16987 ---- Animal proteins ---- Aggrecan core protein Source.81: DFBPPR17003 ---- Animal proteins ---- Toll-like receptor 9 Source.82: DFBPPR17057 ---- Animal proteins ---- Cation-independent mannose-6-phosphate receptor Source.83: DFBPPR17310 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit alpha-1 Source.84: DFBPPR17354 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.85: DFBPPR17375 ---- Animal proteins ---- Peptidyl-glycine alpha-amidating monooxygenase Source.86: DFBPPR17396 ---- Animal proteins ---- Trans-acting T-cell-specific transcription factor GATA-3 Source.87: DFBPPR17536 ---- Animal proteins ---- Protein arginine N-methyltransferase 6 Source.88: DFBPPR17830 ---- Animal proteins ---- Vasopressin V2 receptor Source.89: DFBPPR17854 ---- Animal proteins ---- Tyrosine 3-monooxygenase Source.90: DFBPPR17941 ---- Animal proteins ---- Hepatocyte growth factor-regulated tyrosine kinase substrate Source.91: DFBPPR17996 ---- Animal proteins ---- UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Source.92: DFBPPR18086 ---- Animal proteins ---- N-lysine methyltransferase SMYD2 Source.93: DFBPPR18191 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit alpha-2 Source.94: DFBPPR18522 ---- Animal proteins ---- POU domain, class 5, transcription factor 1 Source.95: DFBPPR18534 ---- Animal proteins ---- M-phase inducer phosphatase 3 Source.96: DFBPPR18535 ---- Animal proteins ---- M-phase inducer phosphatase 1 Source.97: DFBPPR18846 ---- Animal proteins ---- Sex-determining region Y protein Source.98: DFBPPR19018 ---- Animal proteins ---- Interferon regulatory factor 5 Source.99: DFBPPR19031 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit alpha-3 Source.100: DFBPPR19092 ---- Animal proteins ---- Autophagy-related protein 13 Source.101: DFBPPR19113 ---- Animal proteins ---- Glycine amidinotransferase, mitochondrial Source.102: DFBPPR19381 ---- Animal proteins ---- BRCA2 and CDKN1A-interacting protein Source.103: DFBPPR19406 ---- Animal proteins ---- [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial Source.104: DFBPPR19430 ---- Animal proteins ---- Aryl-hydrocarbon-interacting protein-like 1 Source.105: DFBPPR19768 ---- Animal proteins ---- Peroxynitrite isomerase THAP4 Source.106: DFBPPR19792 ---- Animal proteins ---- Cleavage stimulation factor subunit 2 Source.107: DFBPPR19913 ---- Animal proteins ---- NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Source.108: DFBPPR20028 ---- Animal proteins ---- Sine oculis-binding protein homolog Source.109: DFBPPR20095 ---- Animal proteins ---- Putative histone-lysine N-methyltransferase PRDM6 Source.110: DFBPPR20686 ---- Animal proteins ---- Cytochrome c-type heme lyase Source.111: DFBPPR20692 ---- Animal proteins ---- ELMO domain-containing protein 3 Source.112: DFBPPR21108 ---- Animal proteins ---- Pituitary-specific positive transcription factor 1 Source.113: DFBPPR21234 ---- Animal proteins ---- 60S ribosomal protein L4 Source.114: DFBPPR21387 ---- Animal proteins ---- Surfeit locus protein 4 Source.115: DFBPPR21808 ---- Animal proteins ---- Solute carrier family 22 member 7 Source.116: DFBPPR21907 ---- Animal proteins ---- Molybdate-anion transporter Source.117: DFBPPR22015 ---- Animal proteins ---- Solute carrier family 35 member F5 Source.118: DFBPPR22159 ---- Animal proteins ---- Fanconi anemia core complex-associated protein 24 Source.119: DFBPPR22611 ---- Animal proteins ---- Coiled-coil domain-containing protein 105 Source.120: DFBPPR8681 ---- Animal proteins ---- Toll-like receptor 9 Source.121: DFBPPR8682 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.122: DFBPPR9008 ---- Animal proteins ---- Sterol regulatory element-binding protein cleavage-activating protein Source.123: DFBPPR9028 ---- Animal proteins ---- Glycine amidinotransferase, mitochondrial Source.124: DFBPPR9067 ---- Animal proteins ---- N-lysine methyltransferase SMYD2 Source.125: DFBPPR9070 ---- Animal proteins ---- Angiopoietin-related protein 4 Source.126: DFBPPR9169 ---- Animal proteins ---- Aggrecan core protein Source.127: DFBPPR9310 ---- Animal proteins ---- Vasopressin V2 receptor Source.128: DFBPPR9320 ---- Animal proteins ---- Aromatase 1 Source.129: DFBPPR9347 ---- Animal proteins ---- C-reactive protein Source.130: DFBPPR9451 ---- Animal proteins ---- M-phase inducer phosphatase 3 Source.131: DFBPPR9509 ---- Animal proteins ---- Unconventional myosin-VIIa Source.132: DFBPPR9873 ---- Animal proteins ---- Solute carrier family 22 member 7 Source.133: DFBPPR9977 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.134: DFBPPR10118 ---- Animal proteins ---- GATA-binding factor 3 Source.135: DFBPPR10180 ---- Animal proteins ---- Teneurin-2 Source.136: DFBPPR10301 ---- Animal proteins ---- Transcription factor SOX-2 Source.137: DFBPPR10302 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit alpha-1 Source.138: DFBPPR10411 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.139: DFBPPR10416 ---- Animal proteins ---- Ankyrin repeat domain-containing protein 1 Source.140: DFBPPR10452 ---- Animal proteins ---- Desmin Source.141: DFBPPR10503 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.142: DFBPPR10528 ---- Animal proteins ---- Far upstream element-binding protein 2 Source.143: DFBPPR10545 ---- Animal proteins ---- Transcriptional activator GLI3 Source.144: DFBPPR10625 ---- Animal proteins ---- N-lysine methyltransferase SMYD2 Source.145: DFBPPR10703 ---- Animal proteins ---- Tensin Source.146: DFBPPR10759 ---- Animal proteins ---- Phosphoethanolamine/phosphocholine phosphatase Source.147: DFBPPR11082 ---- Animal proteins ---- Protein-glucosylgalactosylhydroxylysine glucosidase Source.148: DFBPPR11093 ---- Animal proteins ---- DBIRD complex subunit ZNF326 Source.149: DFBPPR11132 ---- Animal proteins ---- Glycine amidinotransferase, mitochondrial Source.150: DFBPPR11197 ---- Animal proteins ---- Phosphatase and actin regulator 4 Source.151: DFBPPR11281 ---- Animal proteins ---- LIM domain-binding protein 2 Source.152: DFBPPR11298 ---- Animal proteins ---- Transcription elongation factor SPT5 Source.153: DFBPPR11352 ---- Animal proteins ---- Pituitary-specific positive transcription factor 1 Source.154: DFBPPR11471 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 11 Source.155: DFBPPR11706 ---- Animal proteins ---- Sine oculis-binding protein homolog Source.156: DFBPPR11709 ---- Animal proteins ---- Xin actin-binding repeat-containing protein 1 Source.157: DFBPPR11796 ---- Animal proteins ---- Surfeit locus protein 4 Source.158: DFBPPR11819 ---- Animal proteins ---- Cytochrome c-type heme lyase Source.159: DFBPPR11990 ---- Animal proteins ---- Transcription factor SOX-1 Source.160: DFBPPR12041 ---- Animal proteins ---- Paladin Source.161: DFBPPR12060 ---- Animal proteins ---- Neurobeachin Source.162: DFBPPR12091 ---- Animal proteins ---- Neurofibromin Source.163: DFBPPR12147 ---- Animal proteins ---- Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C Source.164: DFBPPR12262 ---- Animal proteins ---- Zona pellucida sperm-binding protein 4 Source.165: DFBPPR12756 ---- Animal proteins ---- Aromatase Source.166: DFBPPR13143 ---- Animal proteins ---- Toll-like receptor 9 Source.167: DFBPPR13176 ---- Animal proteins ---- Aromatase Source.168: DFBPPR13406 ---- Animal proteins ---- Cortactin-binding protein 2 Source.169: DFBPPR13576 ---- Animal proteins ---- Toll-like receptor 9 Source.170: DFBPPR13643 ---- Animal proteins ---- Transcription factor SOX-2 Source.171: DFBPPR13677 ---- Animal proteins ---- Prolactin receptor Source.172: DFBPPR13910 ---- Animal proteins ---- Pituitary-specific positive transcription factor 1 Source.173: DFBPPR14026 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.174: DFBPPR14060 ---- Animal proteins ---- Gamma-crystallin M2 Source.175: DFBPPR14150 ---- Marine protein ---- NADH-ubiquinone oxidoreductase chain 4 Source.176: DFBPPR14582 ---- Marine protein ---- Tyrosine 3-monooxygenase Source.177: DFBPPR14866 ---- Marine protein ---- Tyrosine 3-monooxygenase Source.178: DFBPPR14886 ---- Microorganism protein ---- Serine/threonine-protein kinase SSN3 Source.179: DFBPPR14937 ---- Microorganism protein ---- Sulfate adenylyltransferase Source.180: DFBPPR14946 ---- Microorganism protein ---- Cytochrome c oxidase subunit 1 Source.181: DFBPPR14982 ---- Microorganism protein ---- Cytochrome P450 monooxygenase PUL2 Source.182: DFBPPR15011 ---- Microorganism protein ---- Cytochrome b Source.183: DFBPPR15550 ---- Microorganism protein ---- COPII coat assembly protein SEC16 Source.184: DFBPPR15637 ---- Microorganism protein ---- Pre-mRNA-splicing factor SLT11 Source.185: DFBPPR15826 ---- Microorganism protein ---- Galactose-1-phosphate uridylyltransferase Source.186: DFBPPR7766 ---- Plant protein ---- Cytochrome c oxidase subunit 1 Source.187: DFBPPR7928 ---- Plant protein ---- Putative cis-zeatin O-glucosyltransferase Source.188: DFBPPR8156 ---- Plant protein ---- Nodulin-30
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity

The antioxidant activity of the synthetic tripeptide was evaluated using a FRAP assay and the activity of glutathione (273.28 ± 12.13 mmol Fe³⁺/mol peptide) was measured as a positive control. The peptide PMH showed low antioxidant activity with a relative antioxidant activity of 0.99 ± 0.03 mmol Fe³⁺/mol peptide (The activity have been reported as the averages of three independent experiments ± standard deviation.).

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	The peptide was synthesized using solid phase Fmoc Chemistry.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The result of the QSAR modeling indicated that the electronic and hydrogen-bonding properties of the amino acids in the tripeptide sequences, as well as the steric properties of the amino acid residues at the C- and N-termini, played an important role in the antioxidant activities of the tripeptides. The antioxidant activities of the tripeptides were generally higher with Cys and Trp amino acid residues in the sequence.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Tian, M., Fang, B., Jiang, L., Guo, H., Cui, J., Ren, F. Structure-activity relationship of a series of antioxidant tripeptides derived from β-Lactoglobulin using QSAR modeling. Dairy Science & Technology. 2015, 95, 451-63. DOI: 10.1007/s13594-015-0226-5
Other literature(s)	N.D
PubDate	2015