Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX0972(Antioxidative peptide)

DFBP ID	DFBPANOX0972
Peptide sequence	CHI
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Cys-His-Ile

Single-letter amino acid

CHI

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	371.46 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.14 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.2667^c

Hydrophilic residue ratio

33.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine whey protein
Precursor protein	β-Lactoglobulin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0859 ---- Plant proteins ---- Glucose-1-phosphate adenylyltransferase small subunit 2, chloroplastic/amyloplastic/cytosolic Source.2: DFBPPR1245 ---- Plant proteins ---- TPR repeat-containing protein ZIP4 Source.3: DFBPPR1275 ---- Plant proteins ---- Transcription factor TIP2 Source.4: DFBPPR1366 ---- Plant proteins ---- Kinesin-like protein KIN-7F Source.5: DFBPPR1427 ---- Plant proteins ---- Probable esterase D14L Source.6: DFBPPR1633 ---- Plant proteins ---- Coronatine-insensitive protein homolog 1a Source.7: DFBPPR1721 ---- Plant proteins ---- Cyclin-dependent kinase C-1 Source.8: DFBPPR1745 ---- Plant proteins ---- Ferredoxin--NADP reductase, root isozyme, chloroplastic Source.9: DFBPPR1805 ---- Plant proteins ---- Probable polyribonucleotide nucleotidyltransferase 1, chloroplastic Source.10: DFBPPR1860 ---- Plant proteins ---- Kinesin-like protein KIN-7A Source.11: DFBPPR2013 ---- Plant proteins ---- Laccase-6 Source.12: DFBPPR2018 ---- Plant proteins ---- Ferredoxin--NADP reductase, embryo isozyme, chloroplastic Source.13: DFBPPR2063 ---- Plant proteins ---- Non-specific lipid-transfer protein C6 Source.14: DFBPPR2311 ---- Plant proteins ---- Histone acetyltransferase GCN5 Source.15: DFBPPR2429 ---- Plant proteins ---- Multicopper oxidase LPR1 homolog 5 Source.16: DFBPPR2442 ---- Plant proteins ---- Vacuolar cation/proton exchanger 1c Source.17: DFBPPR2594 ---- Plant proteins ---- Protein HAPLESS 2-A Source.18: DFBPPR2606 ---- Plant proteins ---- Multicopper oxidase LPR1 homolog 4 Source.19: DFBPPR2633 ---- Plant proteins ---- Protein ADP-ribosyltransferase PARP3 Source.20: DFBPPR2693 ---- Plant proteins ---- Parkeol synthase Source.21: DFBPPR2761 ---- Plant proteins ---- Ent-kaurene synthase-like 3 Source.22: DFBPPR2767 ---- Plant proteins ---- Diacylglycerol O-acyltransferase 1-2 Source.23: DFBPPR2839 ---- Plant proteins ---- Multicopper oxidase LPR1 homolog 2 Source.24: DFBPPR2849 ---- Plant proteins ---- Acyl-CoA-binding domain-containing protein 3 Source.25: DFBPPR2871 ---- Plant proteins ---- Protein SEMI-ROLLED LEAF 2 Source.26: DFBPPR2903 ---- Plant proteins ---- Multicopper oxidase LPR1 homolog 3 Source.27: DFBPPR3061 ---- Plant proteins ---- 50S ribosomal protein L22, chloroplastic Source.28: DFBPPR3127 ---- Plant proteins ---- Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial Source.29: DFBPPR3141 ---- Plant proteins ---- Multicopper oxidase LPR1 homolog 1 Source.30: DFBPPR3623 ---- Plant proteins ---- Kinesin-like protein KIN-14K Source.31: DFBPPR3656 ---- Plant proteins ---- Kinesin-like protein KIN-7C Source.32: DFBPPR3663 ---- Plant proteins ---- Kinesin-like protein KIN-7J Source.33: DFBPPR3805 ---- Plant proteins ---- Putative inactive kinesin-like protein KIN-7B Source.34: DFBPPR3920 ---- Plant proteins ---- Glutaredoxin-C9 Source.35: DFBPPR3940 ---- Plant proteins ---- Putative cyclin-D2-3 Source.36: DFBPPR4028 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 31 Source.37: DFBPPR4243 ---- Plant proteins ---- UDP-glucose:2-hydroxyflavanone C-glucosyltransferase Source.38: DFBPPR4315 ---- Plant proteins ---- Organelle RRM domain-containing protein 1, chloroplastic Source.39: DFBPPR4333 ---- Plant proteins ---- Putative potassium channel KAT5 Source.40: DFBPPR4390 ---- Plant proteins ---- Spermidine hydroxycinnamoyltransferase 2 Source.41: DFBPPR4444 ---- Plant proteins ---- Formin-like protein 6 Source.42: DFBPPR4609 ---- Plant proteins ---- BURP domain-containing protein 16 Source.43: DFBPPR4712 ---- Plant proteins ---- Putative UPF0496 protein 5 Source.44: DFBPPR4727 ---- Plant proteins ---- Putative ripening-related protein 4 Source.45: DFBPPR4749 ---- Plant proteins ---- BURP domain-containing protein 8 Source.46: DFBPPR4802 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 34 Source.47: DFBPPR4877 ---- Plant proteins ---- Putative B3 domain-containing protein Os08g0333500 Source.48: DFBPPR4934 ---- Plant proteins ---- U-box domain-containing protein 70 Source.49: DFBPPR4940 ---- Plant proteins ---- Protein STAY-GREEN, chloroplastic Source.50: DFBPPR5011 ---- Plant proteins ---- Diacylglycerol O-acyltransferase 1A Source.51: DFBPPR5042 ---- Plant proteins ---- Diacylglycerol O-acyltransferase 1B Source.52: DFBPPR5059 ---- Plant proteins ---- Phosphoenolpyruvate carboxylase Source.53: DFBPPR5688 ---- Plant proteins ---- Organelle RRM domain-containing protein 1, chloroplastic Source.54: DFBPPR5699 ---- Plant proteins ---- Sucrose-phosphate synthase Source.55: DFBPPR5852 ---- Plant proteins ---- Sucrose synthase 2 Source.56: DFBPPR5977 ---- Plant proteins ---- Putative AC transposase Source.57: DFBPPR6030 ---- Plant proteins ---- DNA polymerase Source.58: DFBPPR6149 ---- Plant proteins ---- 60S ribosomal protein L17 Source.59: DFBPPR6162 ---- Plant proteins ---- Putative AC9 transposase Source.60: DFBPPR6348 ---- Plant proteins ---- Ferredoxin--NADP reductase, root isozyme, chloroplastic Source.61: DFBPPR6393 ---- Plant proteins ---- Protein STAY-GREEN, chloroplastic Source.62: DFBPPR6401 ---- Plant proteins ---- 50S ribosomal protein L22, chloroplastic Source.63: DFBPPR6479 ---- Plant proteins ---- Non-functional protein STAY-GREEN, chloroplastic Source.64: DFBPPR6669 ---- Plant proteins ---- Fructose-1,6-bisphosphatase, chloroplastic Source.65: DFBPPR6863 ---- Plant proteins ---- Eukaryotic translation initiation factor 1A Source.66: DFBPPR6877 ---- Plant proteins ---- 50S ribosomal protein L22, chloroplastic Source.67: DFBPPR7270 ---- Plant proteins ---- 50S ribosomal protein L22, chloroplastic Source.68: DFBPPR7332 ---- Plant proteins ---- 60S ribosomal protein L17-1 Source.69: DFBPPR7346 ---- Plant proteins ---- 60S ribosomal protein L17-2 Source.70: DFBPPR7645 ---- Milk proteins ---- Kallikrein-6 Source.71: DFBPPR8191 ---- Plant proteins ---- L-ascorbate oxidase Source.72: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin Source.73: DFBPPR16003 ---- Animal proteins ---- Sodium/calcium exchanger 1 Source.74: DFBPPR16024 ---- Animal proteins ---- Podocalyxin Source.75: DFBPPR16211 ---- Animal proteins ---- Lysine-specific demethylase 5C Source.76: DFBPPR16213 ---- Animal proteins ---- Ciliary neurotrophic factor receptor subunit alpha Source.77: DFBPPR16232 ---- Animal proteins ---- Lysine-specific demethylase 5D Source.78: DFBPPR16488 ---- Animal proteins ---- Coagulation factor VIII Source.79: DFBPPR16510 ---- Animal proteins ---- B1 bradykinin receptor Source.80: DFBPPR16511 ---- Animal proteins ---- Neuropeptide Y receptor type 5 Source.81: DFBPPR16623 ---- Animal proteins ---- Lysozyme C, spleen isozyme Source.82: DFBPPR16833 ---- Animal proteins ---- Thiosulfate sulfurtransferase Source.83: DFBPPR16884 ---- Animal proteins ---- Sodium/calcium exchanger 1 Source.84: DFBPPR16953 ---- Animal proteins ---- Activin receptor type-2A Source.85: DFBPPR17164 ---- Animal proteins ---- Beta-2 adrenergic receptor Source.86: DFBPPR17322 ---- Animal proteins ---- Lysyl oxidase homolog 2 Source.87: DFBPPR17426 ---- Animal proteins ---- DNA polymerase delta catalytic subunit Source.88: DFBPPR17433 ---- Animal proteins ---- Caveolin-3 Source.89: DFBPPR17479 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 13 Source.90: DFBPPR17483 ---- Animal proteins ---- Speckle targeted PIP5K1A-regulated poly(A) polymerase Source.91: DFBPPR17567 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL1 Source.92: DFBPPR17802 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP4 Source.93: DFBPPR17829 ---- Animal proteins ---- Thrombospondin-1 Source.94: DFBPPR18140 ---- Animal proteins ---- Semaphorin-3C Source.95: DFBPPR18618 ---- Animal proteins ---- AP-2 complex subunit beta Source.96: DFBPPR18786 ---- Animal proteins ---- Bis(5'-adenosyl)-triphosphatase ENPP4 Source.97: DFBPPR18810 ---- Animal proteins ---- SHC-transforming protein 1 Source.98: DFBPPR18898 ---- Animal proteins ---- Complexin-3 Source.99: DFBPPR18925 ---- Animal proteins ---- Neurochondrin Source.100: DFBPPR18936 ---- Animal proteins ---- S-methyl-5'-thioadenosine phosphorylase Source.101: DFBPPR18943 ---- Animal proteins ---- C-terminal-binding protein 2 Source.102: DFBPPR19265 ---- Animal proteins ---- 28S ribosomal protein S29, mitochondrial Source.103: DFBPPR19427 ---- Animal proteins ---- Probable tRNA(His) guanylyltransferase Source.104: DFBPPR19593 ---- Animal proteins ---- DNA-directed RNA polymerase III subunit RPC1 Source.105: DFBPPR19630 ---- Animal proteins ---- Glycerol kinase Source.106: DFBPPR19686 ---- Animal proteins ---- Spindle and kinetochore-associated protein 1 Source.107: DFBPPR19727 ---- Animal proteins ---- Protein SGT1 homolog Source.108: DFBPPR19847 ---- Animal proteins ---- Eukaryotic translation initiation factor 3 subunit C Source.109: DFBPPR19943 ---- Animal proteins ---- Keratin, type II cytoskeletal 80 Source.110: DFBPPR20097 ---- Animal proteins ---- AP-1 complex subunit beta-1 Source.111: DFBPPR20375 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX56 Source.112: DFBPPR20431 ---- Animal proteins ---- Cell division cycle protein 27 homolog Source.113: DFBPPR20458 ---- Animal proteins ---- Putative transcription factor Ovo-like 1 Source.114: DFBPPR20641 ---- Animal proteins ---- Centrosomal protein of 41 kDa Source.115: DFBPPR20757 ---- Animal proteins ---- F-box only protein 9 Source.116: DFBPPR20820 ---- Animal proteins ---- D-2-hydroxyglutarate dehydrogenase, mitochondrial Source.117: DFBPPR21047 ---- Animal proteins ---- WD repeat-containing protein 48 Source.118: DFBPPR21055 ---- Animal proteins ---- 39S ribosomal protein L37, mitochondrial Source.119: DFBPPR21103 ---- Animal proteins ---- F-box only protein 32 Source.120: DFBPPR21384 ---- Animal proteins ---- Transcription factor Sp2 Source.121: DFBPPR21421 ---- Animal proteins ---- Protein SMG8 Source.122: DFBPPR21479 ---- Animal proteins ---- Pentraxin-related protein PTX3 Source.123: DFBPPR21484 ---- Animal proteins ---- Armadillo repeat-containing protein 8 Source.124: DFBPPR21668 ---- Animal proteins ---- Putative deoxyribonuclease TATDN1 Source.125: DFBPPR21788 ---- Animal proteins ---- Keratin-associated protein 3-3 Source.126: DFBPPR21819 ---- Animal proteins ---- Putative sodium-coupled neutral amino acid transporter 11 Source.127: DFBPPR21853 ---- Animal proteins ---- F-box/LRR-repeat protein 14 Source.128: DFBPPR21886 ---- Animal proteins ---- Interferon-stimulated 20 kDa exonuclease-like 2 Source.129: DFBPPR22275 ---- Animal proteins ---- 60S ribosomal protein L17 Source.130: DFBPPR22351 ---- Animal proteins ---- Transmembrane protein 168 Source.131: DFBPPR22381 ---- Animal proteins ---- F-box only protein 39 Source.132: DFBPPR22634 ---- Animal proteins ---- Testis-expressed protein 30 Source.133: DFBPPR8640 ---- Animal proteins ---- Rho-associated protein kinase 2 Source.134: DFBPPR8726 ---- Animal proteins ---- Serine-protein kinase ATM Source.135: DFBPPR8759 ---- Animal proteins ---- Caveolin-3 Source.136: DFBPPR8984 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.137: DFBPPR9077 ---- Animal proteins ---- Lysozyme C-2 Source.138: DFBPPR9099 ---- Animal proteins ---- Lysozyme C-3 Source.139: DFBPPR9105 ---- Animal proteins ---- Lysine-specific demethylase 5C Source.140: DFBPPR9168 ---- Animal proteins ---- Inhibitor of carbonic anhydrase Source.141: DFBPPR9172 ---- Animal proteins ---- Protein N-terminal asparagine amidohydrolase Source.142: DFBPPR9217 ---- Animal proteins ---- Coagulation factor VIII Source.143: DFBPPR9276 ---- Animal proteins ---- Type I iodothyronine deiodinase Source.144: DFBPPR9291 ---- Animal proteins ---- Lysozyme C-1 Source.145: DFBPPR9393 ---- Animal proteins ---- Neuropeptide Y receptor type 5 Source.146: DFBPPR9548 ---- Animal proteins ---- Membrane progestin receptor alpha Source.147: DFBPPR9785 ---- Animal proteins ---- F-box only protein 32 Source.148: DFBPPR9901 ---- Animal proteins ---- 40S ribosomal protein S14 Source.149: DFBPPR10039 ---- Animal proteins ---- Activin receptor type-2A Source.150: DFBPPR10080 ---- Animal proteins ---- High affinity nerve growth factor receptor Source.151: DFBPPR10196 ---- Animal proteins ---- Transcription factor Sp3 Source.152: DFBPPR10213 ---- Animal proteins ---- Glycerophosphodiester phosphodiesterase domain-containing protein 5 Source.153: DFBPPR10270 ---- Animal proteins ---- Agrin Source.154: DFBPPR10308 ---- Animal proteins ---- DNA (cytosine-5)-methyltransferase 1 Source.155: DFBPPR10539 ---- Animal proteins ---- Netrin receptor UNC5C Source.156: DFBPPR10649 ---- Animal proteins ---- Ciliary neurotrophic factor receptor subunit alpha Source.157: DFBPPR10686 ---- Animal proteins ---- Collectin-11 Source.158: DFBPPR10751 ---- Animal proteins ---- Thiosulfate sulfurtransferase Source.159: DFBPPR10800 ---- Animal proteins ---- DNA polymerase subunit gamma-1 Source.160: DFBPPR10802 ---- Animal proteins ---- Kinesin-like protein KIF18B Source.161: DFBPPR10872 ---- Animal proteins ---- Inactive tyrosine-protein kinase 7 Source.162: DFBPPR10930 ---- Animal proteins ---- WD repeat-containing protein 48 Source.163: DFBPPR10972 ---- Animal proteins ---- Structural maintenance of chromosomes protein 5 Source.164: DFBPPR10983 ---- Animal proteins ---- DNA replication licensing factor MCM3 Source.165: DFBPPR11097 ---- Animal proteins ---- Twinkle protein, mitochondrial Source.166: DFBPPR11201 ---- Animal proteins ---- ATP-dependent RNA helicase DHX30 Source.167: DFBPPR11343 ---- Animal proteins ---- Transcription factor Sp8 Source.168: DFBPPR11409 ---- Animal proteins ---- Transcriptional repressor CTCF Source.169: DFBPPR11571 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.170: DFBPPR11710 ---- Animal proteins ---- WAP four-disulfide core domain protein 1 Source.171: DFBPPR11720 ---- Animal proteins ---- Interleukin-17 receptor D Source.172: DFBPPR11744 ---- Animal proteins ---- Centrosomal protein of 41 kDa Source.173: DFBPPR11878 ---- Animal proteins ---- Prolyl endopeptidase-like Source.174: DFBPPR11898 ---- Animal proteins ---- Serine/threonine-protein phosphatase 6 regulatory subunit 3 Source.175: DFBPPR12118 ---- Animal proteins ---- Protein EURL Source.176: DFBPPR12160 ---- Animal proteins ---- Transmembrane protein 229B Source.177: DFBPPR12275 ---- Animal proteins ---- Ryanodine receptor 3 Source.178: DFBPPR12411 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit epsilon Source.179: DFBPPR12486 ---- Animal proteins ---- Adenylate cyclase type 10 Source.180: DFBPPR12834 ---- Animal proteins ---- B1 bradykinin receptor Source.181: DFBPPR12851 ---- Animal proteins ---- UDP-glucuronosyltransferase 1A4 Source.182: DFBPPR13069 ---- Animal proteins ---- RLA class II histocompatibility antigen, DP alpha-1 chain Source.183: DFBPPR13570 ---- Animal proteins ---- Gap junction alpha-8 protein Source.184: DFBPPR13644 ---- Animal proteins ---- Activin receptor type-2A Source.185: DFBPPR13740 ---- Animal proteins ---- Lysozyme C, kidney isozyme Source.186: DFBPPR13869 ---- Animal proteins ---- Keratin, high sulfur matrix protein, IIIB4 Source.187: DFBPPR14100 ---- Marine protein ---- Cytochrome b Source.188: DFBPPR14242 ---- Marine protein ---- Cytochrome b Source.189: DFBPPR14411 ---- Marine protein ---- 50S ribosomal protein L22, chloroplastic Source.190: DFBPPR14584 ---- Marine protein ---- N-acylneuraminate cytidylyltransferase Source.191: DFBPPR14590 ---- Marine protein ---- Cytochrome b Source.192: DFBPPR14966 ---- Microorganism protein ---- PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Source.193: DFBPPR15015 ---- Microorganism protein ---- Postreplication repair E3 ubiquitin-protein ligase RAD18 Source.194: DFBPPR15021 ---- Microorganism protein ---- Mitochondrial Rho GTPase 1 Source.195: DFBPPR15059 ---- Microorganism protein ---- Iron transport multicopper oxidase FET3 Source.196: DFBPPR15165 ---- Microorganism protein ---- Carbamoyl-phosphate synthase arginine-specific small chain Source.197: DFBPPR15214 ---- Microorganism protein ---- Endoribonuclease YSH1 Source.198: DFBPPR15314 ---- Microorganism protein ---- Probable metalloprotease ARX1 Source.199: DFBPPR15327 ---- Microorganism protein ---- Cytoplasmic tRNA 2-thiolation protein 2 Source.200: DFBPPR15341 ---- Microorganism protein ---- Cytochrome c oxidase subunit 3 Source.201: DFBPPR15856 ---- Microorganism protein ---- Laccase-2 Source.202: DFBPPR15857 ---- Microorganism protein ---- Laccase-1 Source.203: DFBPPR7964 ---- Plant protein ---- Sucrose synthase Source.204: DFBPPR8296 ---- Plant protein ---- 50S ribosomal protein L22, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity

The antioxidant activity of the synthetic tripeptide was evaluated using a FRAP assay and the activity of glutathione (273.28 ± 12.13 mmol Fe³⁺/mol peptide) was measured as a positive control. The peptide CHI showed moderate antioxidant activity with a relative antioxidant activity of 28.07 ± 1.15 mmol Fe³⁺/mol peptide (The activity have been reported as the averages of three independent experiments ± standard deviation.).

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CS)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	The peptide was synthesized using solid phase Fmoc Chemistry.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The result of the QSAR modeling indicated that the electronic and hydrogen-bonding properties of the amino acids in the tripeptide sequences, as well as the steric properties of the amino acid residues at the C- and N-termini, played an important role in the antioxidant activities of the tripeptides. The antioxidant activities of the tripeptides were generally higher with Cys and Trp amino acid residues in the sequence.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Tian, M., Fang, B., Jiang, L., Guo, H., Cui, J., Ren, F. Structure-activity relationship of a series of antioxidant tripeptides derived from β-Lactoglobulin using QSAR modeling. Dairy Science & Technology. 2015, 95, 451-63. DOI: 10.1007/s13594-015-0226-5
Other literature(s)	N.D
PubDate	2015