Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX1133(Antioxidative peptide)

DFBP ID	DFBPANOX1133
Peptide sequence	VLPVP
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Val-Leu-Pro-Val-Pro

Single-letter amino acid

VLPVP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
523.33 Da	523.67 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.8000^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protiens, Bovine
Precursor protein	β-Casein
Residue position	f(170-174)
Precursor protein(s) search	Source.1: DFBPPR1386 ---- Plant proteins ---- Transcription factor LATE FLOWERING Source.2: DFBPPR1641 ---- Plant proteins ---- Enolase Source.3: DFBPPR2731 ---- Plant proteins ---- Eukaryotic translation initiation factor 4G Source.4: DFBPPR3125 ---- Plant proteins ---- Putative alpha-L-fucosidase 1 Source.5: DFBPPR5650 ---- Plant proteins ---- Enolase 1 Source.6: DFBPPR5675 ---- Plant proteins ---- Enolase 2 Source.7: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.8: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.9: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.10: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.11: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.12: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.13: DFBPPR16885 ---- Animal proteins ---- cGMP-dependent protein kinase 1 Source.14: DFBPPR17263 ---- Animal proteins ---- E3 ubiquitin-protein ligase UHRF1 Source.15: DFBPPR17377 ---- Animal proteins ---- Adenylate cyclase type 1 Source.16: DFBPPR19814 ---- Animal proteins ---- Protein delta homolog 2 Source.17: DFBPPR9207 ---- Animal proteins ---- Beta-enolase Source.18: DFBPPR9738 ---- Animal proteins ---- Protein delta homolog 2 Source.19: DFBPPR12360 ---- Animal proteins ---- cGMP-dependent protein kinase 1 Source.20: DFBPPR12425 ---- Animal proteins ---- Beta-enolase Source.21: DFBPPR14187 ---- Marine protein ---- Secreted phosphoprotein 24 Source.22: DFBPPR15045 ---- Microorganism protein ---- Enolase Source.23: DFBPPR7767 ---- Plant protein ---- Adenylosuccinate synthetase 2, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity

The peptide VLPVP (pep 2) exhibited lesser antioxidative potential by chemical method and in-vitro hydrogen peroxide induced oxidative stress conditions generated in fibroblast cells compared to VLPVPQK (pep 1).

2020(2).png

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	Peptide VLPVP were custom synthesized with purity > 98%, molecular weight, 779.48 from Link Biotech, New Delhi, India. VLPVP was generated from parent antioxidative peptide, VLPVPQK.

Stability & Cytotoxicity

Peptide stability

Literature report:

Table 1. Difference in the availability of amino acid after digestion by proteases in-silico for VLPVP.
Serial No	Name of enzyme	No. of cleavages	Positions of cleavage
1	Pepsin (pH 1.3)	1	2
2	Pepsin (pH > 2)	1	2
3	Proteinase K	3	1,2,4
4	Caspase 1-10	-	-

EHP-Tool:

Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1318
[D2]	DFBPANHY0907
[D3]	DFBPMUFU0321

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Devi, S., Pal, G.K., Kapila, R., Kapila, S. C-terminal sequence deletion effect on antioxidative characteristics of VLPVPQK bioactive peptide from buffalo milk casein. Lwt. 2020, 119. DOI: 10.1016/j.lwt.2019.108816
Other literature(s)	[1] Rival, S.G., et al., Caseins and casein hydrolysates. 1. Lipoxygenase inhibitory properties. J Agric Food Chem, 2001. 49(1): p. 287-294.
PubDate	2020