Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX1166(Antioxidative peptide)

DFBP ID	DFBPANOX1166
Peptide sequence	CSGD
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Cys-Ser-Gly-Asp

Single-letter amino acid

CSGD

Peptide length

Peptide mass

Experimental mass	Theoretical mass
380.10 Da	380.37 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.13 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5500^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Mung bean
Precursor protein	Mung bean meal protein hydrolysate
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1504 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 50 Source.2: DFBPPR1779 ---- Plant proteins ---- SPX domain-containing protein 3 Source.3: DFBPPR1936 ---- Plant proteins ---- tRNA wybutosine-synthesizing protein 2/3/4 Source.4: DFBPPR2024 ---- Plant proteins ---- Methylthioribose-1-phosphate isomerase Source.5: DFBPPR2280 ---- Plant proteins ---- Origin of replication complex subunit 3 Source.6: DFBPPR2703 ---- Plant proteins ---- Homeobox protein knotted-1-like 10 Source.7: DFBPPR3130 ---- Plant proteins ---- COP9 signalosome complex subunit 6 Source.8: DFBPPR3193 ---- Plant proteins ---- Potassium channel AKT3 Source.9: DFBPPR3612 ---- Plant proteins ---- Kinesin-like protein KIN-6 Source.10: DFBPPR3675 ---- Plant proteins ---- Neutral/alkaline invertase 3, chloroplastic Source.11: DFBPPR3913 ---- Plant proteins ---- Serine decarboxylase 2 Source.12: DFBPPR5105 ---- Plant proteins ---- Probable bifunctional TENA-E protein Source.13: DFBPPR6239 ---- Plant proteins ---- Vacuolar-sorting receptor 1 Source.14: DFBPPR6466 ---- Plant proteins ---- Arginine decarboxylase Source.15: DFBPPR7068 ---- Plant proteins ---- Peroxidase 2 Source.16: DFBPPR16644 ---- Animal proteins ---- Arylsulfatase H Source.17: DFBPPR17079 ---- Animal proteins ---- Long-chain fatty acid transport protein 1 Source.18: DFBPPR17893 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 3 Source.19: DFBPPR17949 ---- Animal proteins ---- Insulinoma-associated protein 1 Source.20: DFBPPR18877 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.21: DFBPPR18897 ---- Animal proteins ---- Kelch-like protein 20 Source.22: DFBPPR19855 ---- Animal proteins ---- AP-3 complex subunit mu-1 Source.23: DFBPPR19905 ---- Animal proteins ---- Complement component C6 Source.24: DFBPPR20728 ---- Animal proteins ---- Serine protease 45 Source.25: DFBPPR20806 ---- Animal proteins ---- Transcriptional adapter 1 Source.26: DFBPPR21452 ---- Animal proteins ---- Spermatogenic leucine zipper protein 1 Source.27: DFBPPR21893 ---- Animal proteins ---- Somatomedin-B and thrombospondin type-1 domain-containing protein Source.28: DFBPPR8565 ---- Animal proteins ---- Villin-1 Source.29: DFBPPR8730 ---- Animal proteins ---- Phosphoacetylglucosamine mutase Source.30: DFBPPR8887 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.31: DFBPPR9270 ---- Animal proteins ---- Complement C1s subcomponent Source.32: DFBPPR10043 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.33: DFBPPR10175 ---- Animal proteins ---- Platelet-derived growth factor receptor alpha Source.34: DFBPPR10183 ---- Animal proteins ---- Villin-1 Source.35: DFBPPR10356 ---- Animal proteins ---- RNA cytosine-C(5)-methyltransferase NSUN2 Source.36: DFBPPR10984 ---- Animal proteins ---- Kelch-like protein 20 Source.37: DFBPPR11232 ---- Animal proteins ---- AP-3 complex subunit mu-1 Source.38: DFBPPR11705 ---- Animal proteins ---- SCO-spondin Source.39: DFBPPR11736 ---- Animal proteins ---- Ig lambda chain V-1 region Source.40: DFBPPR12816 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.41: DFBPPR12845 ---- Animal proteins ---- Complement component C8 alpha chain Source.42: DFBPPR14123 ---- Marine protein ---- Albumin 1 Source.43: DFBPPR14124 ---- Marine protein ---- Albumin 2 Source.44: DFBPPR14183 ---- Marine protein ---- Pleckstrin homology-like domain family A member 2 Source.45: DFBPPR14564 ---- Marine protein ---- Piwi-like protein 2 Source.46: DFBPPR14754 ---- Marine protein ---- Clotting factor C Source.47: DFBPPR15485 ---- Microorganism protein ---- Pre-mRNA-splicing factor PRP46
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	(1) The peptide CSGD showed DPPH radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity and metal chelating activity with EC₅₀ values of 0.30, 0.78, 0.04 and 0.26 mg/mL, respectively. (2) The peptide CSGD also showed ferric reducing antioxidant power with the value of 0.16 mM Fe²⁺ reduced/mg peptide.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Defatted mung bean meal was hydrolyzed using 15% (w/w) bromelain (enzyme activity = 97,540 CDU) at pH 6.0 and temperature 50 ◦C for 12 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Sonklin C, Alashi AM, Laohakunjit N, Aluko RE. Functional Characterization of Mung Bean Meal Protein-Derived Antioxidant Peptides. Molecules. 2021 Mar 10;26(6):1515. PMID: PMC7999109 DOI: 10.3390/molecules26061515
Other literature(s)	N.D
PubDate	2021