Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX1174(Antioxidative peptide)

DFBP ID	DFBPANOX1174
Peptide sequence	MKW
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Met-Lys-Trp

Single-letter amino acid

MKW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	463.60 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9667^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Food source
Organism/Source	Protein powder (α-lactoalbumin, casein, rice bran protein, glutenin and hemp seed protein)
Precursor protein	N.D
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0839 ---- Plant proteins ---- Flavone 3'-O-methyltransferase 1 Source.2: DFBPPR0959 ---- Plant proteins ---- Probable serine/threonine-protein kinase BSK3 Source.3: DFBPPR1423 ---- Plant proteins ---- Acetyl-CoA carboxylase 2 Source.4: DFBPPR1958 ---- Plant proteins ---- Phospholipase D alpha 2 Source.5: DFBPPR1990 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 38 Source.6: DFBPPR2074 ---- Plant proteins ---- Potassium transporter 7 Source.7: DFBPPR2135 ---- Plant proteins ---- Putative DNA ligase 4 Source.8: DFBPPR2522 ---- Plant proteins ---- Puromycin-sensitive aminopeptidase Source.9: DFBPPR2550 ---- Plant proteins ---- Polyamine transporter PUT1 Source.10: DFBPPR2832 ---- Plant proteins ---- Probable histone acetyltransferase HAC-like 1 Source.11: DFBPPR3033 ---- Plant proteins ---- Putative beta-glucosidase 17 Source.12: DFBPPR3064 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL9 Source.13: DFBPPR3530 ---- Plant proteins ---- Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOGL2 Source.14: DFBPPR4045 ---- Plant proteins ---- 60S ribosomal protein L11 Source.15: DFBPPR4581 ---- Plant proteins ---- LIMR family protein Os06g0128200 Source.16: DFBPPR4591 ---- Plant proteins ---- Uncharacterized protein ycf76 Source.17: DFBPPR4624 ---- Plant proteins ---- Actin-depolymerizing factor 5 Source.18: DFBPPR4879 ---- Plant proteins ---- Putative zinc finger CCCH domain-containing protein 58 Source.19: DFBPPR4902 ---- Plant proteins ---- Endoribonuclease Dicer homolog 1 Source.20: DFBPPR5221 ---- Plant proteins ---- Protein TIC 214 Source.21: DFBPPR5386 ---- Plant proteins ---- (S)-beta-macrocarpene synthase Source.22: DFBPPR5405 ---- Plant proteins ---- Terpene synthase 2, chloroplastic Source.23: DFBPPR5481 ---- Plant proteins ---- 4-hydroxy-tetrahydrodipicolinate synthase, chloroplastic Source.24: DFBPPR5483 ---- Plant proteins ---- Caffeic acid 3-O-methyltransferase Source.25: DFBPPR5604 ---- Plant proteins ---- Poly [ADP-ribose] polymerase 1 Source.26: DFBPPR5682 ---- Plant proteins ---- Probable terpene synthase 3, chloroplastic Source.27: DFBPPR5685 ---- Plant proteins ---- Protein ABERRANT POLLEN TRANSMISSION 1 Source.28: DFBPPR6146 ---- Plant proteins ---- Uncharacterized protein ycf76 Source.29: DFBPPR6493 ---- Plant proteins ---- Serine carboxypeptidase-like Source.30: DFBPPR6632 ---- Plant proteins ---- Tricetin 3',4',5'-O-trimethyltransferase Source.31: DFBPPR6644 ---- Plant proteins ---- Flavone O-methyltransferase 1 Source.32: DFBPPR6662 ---- Plant proteins ---- 4-hydroxy-tetrahydrodipicolinate synthase 2, chloroplastic Source.33: DFBPPR6663 ---- Plant proteins ---- 4-hydroxy-tetrahydrodipicolinate synthase 1, chloroplastic Source.34: DFBPPR7641 ---- Milk proteins ---- Lactase-phlorizin hydrolase Source.35: DFBPPR16039 ---- Animal proteins ---- Breast cancer type 1 susceptibility protein homolog Source.36: DFBPPR16189 ---- Animal proteins ---- Albumin Source.37: DFBPPR16223 ---- Animal proteins ---- Actin-histidine N-methyltransferase Source.38: DFBPPR16485 ---- Animal proteins ---- Cathepsin S Source.39: DFBPPR16519 ---- Animal proteins ---- Palmitoyltransferase ZDHHC8 Source.40: DFBPPR16547 ---- Animal proteins ---- Alpha-fetoprotein Source.41: DFBPPR16561 ---- Animal proteins ---- UDP-glucuronosyltransferase 2B31 Source.42: DFBPPR16573 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.43: DFBPPR16582 ---- Animal proteins ---- Pepsin A Source.44: DFBPPR16610 ---- Animal proteins ---- Thiopurine S-methyltransferase Source.45: DFBPPR16726 ---- Animal proteins ---- Ral guanine nucleotide dissociation stimulator-like 2 Source.46: DFBPPR16797 ---- Animal proteins ---- Albumin Source.47: DFBPPR16836 ---- Animal proteins ---- Fibronectin Source.48: DFBPPR16935 ---- Animal proteins ---- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Source.49: DFBPPR16941 ---- Animal proteins ---- Sodium/potassium/calcium exchanger 1 Source.50: DFBPPR16951 ---- Animal proteins ---- Prostaglandin E synthase 2 Source.51: DFBPPR16970 ---- Animal proteins ---- Galactoside alpha-(1,2)-fucosyltransferase 1 Source.52: DFBPPR17032 ---- Animal proteins ---- Gamma-aminobutyric acid receptor-associated protein-like 2 Source.53: DFBPPR17374 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 1 Source.54: DFBPPR17831 ---- Animal proteins ---- Histone-lysine N-methyltransferase KMT5B Source.55: DFBPPR17995 ---- Animal proteins ---- Ferrochelatase, mitochondrial Source.56: DFBPPR18192 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.57: DFBPPR18202 ---- Animal proteins ---- Pregnancy-associated glycoprotein 1 Source.58: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.59: DFBPPR18807 ---- Animal proteins ---- Pregnancy-associated glycoprotein 2 Source.60: DFBPPR18844 ---- Animal proteins ---- Phosphatidylinositol 4-kinase alpha Source.61: DFBPPR18848 ---- Animal proteins ---- Ras-related protein Rab-15 Source.62: DFBPPR18902 ---- Animal proteins ---- Plasmanylethanolamine desaturase Source.63: DFBPPR19029 ---- Animal proteins ---- Homeobox protein Hox-B4 Source.64: DFBPPR19125 ---- Animal proteins ---- Alpha-fetoprotein Source.65: DFBPPR19135 ---- Animal proteins ---- Palmitoyltransferase ZDHHC5 Source.66: DFBPPR19495 ---- Animal proteins ---- 28S ribosomal protein S7, mitochondrial Source.67: DFBPPR20068 ---- Animal proteins ---- H2.0-like homeobox protein Source.68: DFBPPR20335 ---- Animal proteins ---- Ubiquitin-like domain-containing CTD phosphatase 1 Source.69: DFBPPR20435 ---- Animal proteins ---- Leucine-rich glioma-inactivated protein 1 Source.70: DFBPPR20577 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor-interacting protein Source.71: DFBPPR20694 ---- Animal proteins ---- Sodium- and chloride-dependent creatine transporter 1 Source.72: DFBPPR20846 ---- Animal proteins ---- Thiopurine S-methyltransferase Source.73: DFBPPR21178 ---- Animal proteins ---- Probable UDP-sugar transporter protein SLC35A5 Source.74: DFBPPR21255 ---- Animal proteins ---- Homeobox protein Hox-B7 Source.75: DFBPPR21333 ---- Animal proteins ---- DDB1- and CUL4-associated factor 15 Source.76: DFBPPR21492 ---- Animal proteins ---- Homeobox protein DBX1 Source.77: DFBPPR21638 ---- Animal proteins ---- 28S ribosomal protein S10, mitochondrial Source.78: DFBPPR21744 ---- Animal proteins ---- Anaphase-promoting complex subunit 13 Source.79: DFBPPR21755 ---- Animal proteins ---- ELMO domain-containing protein 2 Source.80: DFBPPR22111 ---- Animal proteins ---- Homeobox protein Hox-A5 Source.81: DFBPPR22182 ---- Animal proteins ---- Protein TOPAZ1 Source.82: DFBPPR22195 ---- Animal proteins ---- Homeobox protein DBX2 Source.83: DFBPPR22255 ---- Animal proteins ---- Rab9 effector protein with kelch motifs Source.84: DFBPPR8680 ---- Animal proteins ---- Wilms tumor protein homolog Source.85: DFBPPR8726 ---- Animal proteins ---- Serine-protein kinase ATM Source.86: DFBPPR8843 ---- Animal proteins ---- Pepsin A Source.87: DFBPPR9044 ---- Animal proteins ---- Albumin Source.88: DFBPPR9362 ---- Animal proteins ---- Alpha-fetoprotein Source.89: DFBPPR9525 ---- Animal proteins ---- Pregnancy-associated glycoprotein 1 Source.90: DFBPPR9588 ---- Animal proteins ---- ATP synthase protein 8 Source.91: DFBPPR9645 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.92: DFBPPR9676 ---- Animal proteins ---- Pregnancy-associated glycoprotein 2 Source.93: DFBPPR9706 ---- Animal proteins ---- Homeobox protein Hox-B8 Source.94: DFBPPR10045 ---- Animal proteins ---- Albumin Source.95: DFBPPR10125 ---- Animal proteins ---- Leucine-rich repeat transmembrane protein FLRT3 Source.96: DFBPPR10612 ---- Animal proteins ---- Carboxypeptidase Z Source.97: DFBPPR10636 ---- Animal proteins ---- LARGE xylosyl- and glucuronyltransferase 1 Source.98: DFBPPR10693 ---- Animal proteins ---- Ferrochelatase, mitochondrial Source.99: DFBPPR10734 ---- Animal proteins ---- Homeobox protein Hox-B4 Source.100: DFBPPR10800 ---- Animal proteins ---- DNA polymerase subunit gamma-1 Source.101: DFBPPR10918 ---- Animal proteins ---- Frizzled-2 Source.102: DFBPPR10927 ---- Animal proteins ---- Frizzled-7 Source.103: DFBPPR11079 ---- Animal proteins ---- Frizzled-1 Source.104: DFBPPR11139 ---- Animal proteins ---- Homeobox protein Hox-A7 Source.105: DFBPPR11228 ---- Animal proteins ---- CTP synthase 2 Source.106: DFBPPR11294 ---- Animal proteins ---- Frizzled-8 Source.107: DFBPPR11322 ---- Animal proteins ---- Homeobox protein Hox-D4 Source.108: DFBPPR11331 ---- Animal proteins ---- Homeobox protein Hox-A4 Source.109: DFBPPR11457 ---- Animal proteins ---- Homeobox protein DBX2 Source.110: DFBPPR11506 ---- Animal proteins ---- Homeobox protein BarH-like 1b Source.111: DFBPPR11569 ---- Animal proteins ---- Homeobox protein Hox-D8 Source.112: DFBPPR11632 ---- Animal proteins ---- Ubiquitin-like domain-containing CTD phosphatase 1 Source.113: DFBPPR11718 ---- Animal proteins ---- Homeobox protein Hox-C8 Source.114: DFBPPR11742 ---- Animal proteins ---- 28S ribosomal protein S7, mitochondrial Source.115: DFBPPR11799 ---- Animal proteins ---- Homeobox protein Hox-B5 Source.116: DFBPPR11806 ---- Animal proteins ---- Homeobox protein Hox-B6 Source.117: DFBPPR11810 ---- Animal proteins ---- Homeobox protein BarH-like 1 Source.118: DFBPPR11996 ---- Animal proteins ---- Homeobox protein Hox-A6 Source.119: DFBPPR12051 ---- Animal proteins ---- GATOR complex protein WDR24 Source.120: DFBPPR12083 ---- Animal proteins ---- Homeobox protein Hox-A5 Source.121: DFBPPR12165 ---- Animal proteins ---- Vacuolar fusion protein MON1 homolog A Source.122: DFBPPR12234 ---- Animal proteins ---- Rab9 effector protein with kelch motifs Source.123: DFBPPR12357 ---- Animal proteins ---- Galactoside alpha-(1,2)-fucosyltransferase 1 Source.124: DFBPPR12453 ---- Animal proteins ---- Lactase-phlorizin hydrolase Source.125: DFBPPR12536 ---- Animal proteins ---- Albumin Source.126: DFBPPR12748 ---- Animal proteins ---- Pepsin II-1 Source.127: DFBPPR12755 ---- Animal proteins ---- Pepsin II-2/3 Source.128: DFBPPR12757 ---- Animal proteins ---- Pepsin II-4 Source.129: DFBPPR12768 ---- Animal proteins ---- Pepsin-3 Source.130: DFBPPR12779 ---- Animal proteins ---- T-cell surface glycoprotein CD3 zeta chain Source.131: DFBPPR12821 ---- Animal proteins ---- Gastricsin Source.132: DFBPPR12823 ---- Animal proteins ---- Pepsin F Source.133: DFBPPR12949 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.134: DFBPPR12987 ---- Animal proteins ---- Sodium- and chloride-dependent creatine transporter 1 Source.135: DFBPPR13042 ---- Animal proteins ---- Thiopurine S-methyltransferase Source.136: DFBPPR13195 ---- Animal proteins ---- Albumin Source.137: DFBPPR13294 ---- Animal proteins ---- Alpha-fetoprotein Source.138: DFBPPR13365 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.139: DFBPPR13377 ---- Animal proteins ---- Pregnancy-associated glycoprotein Source.140: DFBPPR13394 ---- Animal proteins ---- Thiopurine S-methyltransferase Source.141: DFBPPR13500 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.142: DFBPPR13681 ---- Animal proteins ---- Pregnancy-associated glycoprotein 1 Source.143: DFBPPR13685 ---- Animal proteins ---- T-cell surface glycoprotein CD3 zeta chain Source.144: DFBPPR13788 ---- Animal proteins ---- Albumin Source.145: DFBPPR13798 ---- Animal proteins ---- Pregnancy-associated glycoprotein 6 Source.146: DFBPPR13801 ---- Animal proteins ---- Pregnancy-associated glycoprotein 4 Source.147: DFBPPR13846 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.148: DFBPPR13914 ---- Animal proteins ---- Homeobox protein Hox-C6 Source.149: DFBPPR13925 ---- Animal proteins ---- Homeobox protein BarH-like 2 Source.150: DFBPPR13929 ---- Animal proteins ---- Homeobox protein Hox-A4 Source.151: DFBPPR13930 ---- Animal proteins ---- Homeobox protein Hox-A7 Source.152: DFBPPR13950 ---- Animal proteins ---- Protein TOPAZ1 Source.153: DFBPPR13952 ---- Animal proteins ---- Homeobox protein Hox-A5 Source.154: DFBPPR14167 ---- Marine protein ---- Actin-related protein 8 Source.155: DFBPPR14173 ---- Marine protein ---- Homeobox protein Hox-A5 Source.156: DFBPPR14187 ---- Marine protein ---- Secreted phosphoprotein 24 Source.157: DFBPPR14192 ---- Marine protein ---- Homeobox protein Hox-A7 Source.158: DFBPPR14193 ---- Marine protein ---- ER membrane protein complex subunit 4 Source.159: DFBPPR14563 ---- Marine protein ---- Beta-2 adrenergic receptor Source.160: DFBPPR14716 ---- Marine protein ---- Secreted phosphoprotein 24 Source.161: DFBPPR14808 ---- Marine protein ---- Pseudohemocyanin-1 Source.162: DFBPPR14895 ---- Microorganism protein ---- Chromatin-remodeling ATPase INO80 Source.163: DFBPPR15049 ---- Microorganism protein ---- Ubiquitin-like modifier-activating enzyme ATG7 Source.164: DFBPPR15086 ---- Microorganism protein ---- Pheromone-processing carboxypeptidase KEX1 Source.165: DFBPPR15419 ---- Microorganism protein ---- Mitochondrial distribution and morphology protein 34 Source.166: DFBPPR15469 ---- Microorganism protein ---- SWR1-complex protein 4 Source.167: DFBPPR15753 ---- Microorganism protein ---- KNR4/SMI1 homolog Source.168: DFBPPR15774 ---- Microorganism protein ---- Protein SIA1 Source.169: DFBPPR15858 ---- Microorganism protein ---- Endo-1,4-beta-xylanase Source.170: DFBPPR8120 ---- Plant protein ---- Protein TIC 214 Source.171: DFBPPR8304 ---- Plant protein ---- Protein TIC 214
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide MKW showed ABTS^⋅+ scavenging activity with the value of 4.59 μmol TE/μmol.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The protein powder (α-lactoalbumin, casein, rice bran protein, glutenin and hemp seed protein) was dispersed in deionized water (1:50, w/v) incubated at 37 ℃ and treated with pepsin, pepsin/protein ratio = 5% w/w at pH 2 for 120 min.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yin JY, Han YN, Liu MQ, Piao ZH, Zhang X, Xue YT, Zhang YH. Structure-guided discovery of antioxidant peptides bounded to the Keap1 receptor as hunter for potential dietary antioxidants. Food Chem. 2022 Mar 30;373(Pt A):130999. PMID: 34710694 DOI: 10.1016/j.foodchem.2021.130999
Other literature(s)	N.D
PubDate	2021