Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX1179(Antioxidative peptide)

DFBP ID	DFBPANOX1179
Peptide sequence	CNW
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Cys-Asn-Trp

Single-letter amino acid

CNW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	421.48 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.33 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6333^c

Hydrophilic residue ratio

33.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Food source
Organism/Source	Protein powder (α-lactoalbumin, casein, rice bran protein, glutenin and hemp seed protein)
Precursor protein	N.D
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0934 ---- Plant proteins ---- LRR receptor-like serine/threonine-protein kinase FLS2 Source.2: DFBPPR1000 ---- Plant proteins ---- Flowering time control protein FCA Source.3: DFBPPR1161 ---- Plant proteins ---- Photosystem II protein D1 Source.4: DFBPPR1481 ---- Plant proteins ---- Leucine-rich repeat receptor protein kinase MSL1 Source.5: DFBPPR1743 ---- Plant proteins ---- Phosphatidylinositol 4-phosphate 5-kinase 1 Source.6: DFBPPR2524 ---- Plant proteins ---- Serine carboxypeptidase 3 Source.7: DFBPPR2829 ---- Plant proteins ---- CDP-diacylglycerol--serine O-phosphatidyltransferase 2 Source.8: DFBPPR2863 ---- Plant proteins ---- Serine carboxypeptidase-like Source.9: DFBPPR2949 ---- Plant proteins ---- CDP-diacylglycerol--serine O-phosphatidyltransferase 1 Source.10: DFBPPR3102 ---- Plant proteins ---- Expansin-B18 Source.11: DFBPPR3206 ---- Plant proteins ---- Expansin-B15 Source.12: DFBPPR3267 ---- Plant proteins ---- CDP-diacylglycerol--serine O-phosphatidyltransferase 3 Source.13: DFBPPR3529 ---- Plant proteins ---- Probable E3 ubiquitin-protein ligase XBOS36 Source.14: DFBPPR4324 ---- Plant proteins ---- Elongation factor Ts, mitochondrial Source.15: DFBPPR4401 ---- Plant proteins ---- Phospholipase A1-II 2 Source.16: DFBPPR4993 ---- Plant proteins ---- Photosystem II protein D1 Source.17: DFBPPR5075 ---- Plant proteins ---- DNA polymerase delta catalytic subunit Source.18: DFBPPR5141 ---- Plant proteins ---- Flavonoid 4'-O-methyltransferase Source.19: DFBPPR5394 ---- Plant proteins ---- Photosystem II protein D1 Source.20: DFBPPR6058 ---- Plant proteins ---- Elongation factor Ts, mitochondrial Source.21: DFBPPR6220 ---- Plant proteins ---- Photosystem II protein D1 Source.22: DFBPPR6493 ---- Plant proteins ---- Serine carboxypeptidase-like Source.23: DFBPPR6542 ---- Plant proteins ---- Maturase K Source.24: DFBPPR6648 ---- Plant proteins ---- Photosystem II protein D1 Source.25: DFBPPR6759 ---- Plant proteins ---- Serine carboxypeptidase 3 Source.26: DFBPPR7010 ---- Plant proteins ---- Serine carboxypeptidase 3 Source.27: DFBPPR7023 ---- Plant proteins ---- Photosystem II protein D1 Source.28: DFBPPR7401 ---- Plant proteins ---- Photosystem II protein D1 Source.29: DFBPPR8445 ---- Plant proteins ---- Maturase K Source.30: DFBPPR8452 ---- Plant proteins ---- Photosystem II protein D1 Source.31: DFBPPR8514 ---- Milk proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 3 Source.32: DFBPPR15991 ---- Animal proteins ---- Toll-like receptor 9 Source.33: DFBPPR16128 ---- Animal proteins ---- Cubilin Source.34: DFBPPR16293 ---- Animal proteins ---- Baculoviral IAP repeat-containing protein 3 Source.35: DFBPPR16436 ---- Animal proteins ---- Glycogen debranching enzyme Source.36: DFBPPR16887 ---- Animal proteins ---- Pleiotrophin Source.37: DFBPPR17003 ---- Animal proteins ---- Toll-like receptor 9 Source.38: DFBPPR17337 ---- Animal proteins ---- Polyunsaturated fatty acid lipoxygenase ALOX15 Source.39: DFBPPR17374 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 1 Source.40: DFBPPR17426 ---- Animal proteins ---- DNA polymerase delta catalytic subunit Source.41: DFBPPR17578 ---- Animal proteins ---- Acidic mammalian chitinase Source.42: DFBPPR17733 ---- Animal proteins ---- Protein phosphatase 1B Source.43: DFBPPR18260 ---- Animal proteins ---- 2-oxoglutarate dehydrogenase, mitochondrial Source.44: DFBPPR19801 ---- Animal proteins ---- Outer dense fiber protein 2 Source.45: DFBPPR19905 ---- Animal proteins ---- Complement component C6 Source.46: DFBPPR20244 ---- Animal proteins ---- Zinc finger FYVE domain-containing protein 26 Source.47: DFBPPR20272 ---- Animal proteins ---- Fatty acyl-CoA reductase 2 Source.48: DFBPPR21277 ---- Animal proteins ---- Glucose-6-phosphate exchanger SLC37A2 Source.49: DFBPPR21384 ---- Animal proteins ---- Transcription factor Sp2 Source.50: DFBPPR8544 ---- Animal proteins ---- Xaa-Pro aminopeptidase 2 Source.51: DFBPPR8556 ---- Animal proteins ---- Polyunsaturated fatty acid lipoxygenase ALOX15 Source.52: DFBPPR8616 ---- Animal proteins ---- Pleiotrophin Source.53: DFBPPR8635 ---- Animal proteins ---- Mu-type opioid receptor Source.54: DFBPPR8681 ---- Animal proteins ---- Toll-like receptor 9 Source.55: DFBPPR8923 ---- Animal proteins ---- Cubilin Source.56: DFBPPR9195 ---- Animal proteins ---- Sex-determining region Y protein Source.57: DFBPPR9742 ---- Animal proteins ---- Putative inhibitor of apoptosis Source.58: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin Source.59: DFBPPR10064 ---- Animal proteins ---- Pleiotrophin Source.60: DFBPPR10158 ---- Animal proteins ---- B-cell lymphoma 6 protein homolog Source.61: DFBPPR10173 ---- Animal proteins ---- Teneurin-1 Source.62: DFBPPR10196 ---- Animal proteins ---- Transcription factor Sp3 Source.63: DFBPPR10376 ---- Animal proteins ---- Metalloproteinase inhibitor 3 Source.64: DFBPPR10648 ---- Animal proteins ---- PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Source.65: DFBPPR10804 ---- Animal proteins ---- Integrin alpha-V Source.66: DFBPPR10833 ---- Animal proteins ---- Putative helicase MOV-10 Source.67: DFBPPR10988 ---- Animal proteins ---- Midkine Source.68: DFBPPR11083 ---- Animal proteins ---- Solute carrier family 2, facilitated glucose transporter member 2 Source.69: DFBPPR11221 ---- Animal proteins ---- Zinc finger homeobox protein 4 Source.70: DFBPPR11343 ---- Animal proteins ---- Transcription factor Sp8 Source.71: DFBPPR11356 ---- Animal proteins ---- Homeobox protein AKR Source.72: DFBPPR11469 ---- Animal proteins ---- Cotranscriptional regulator FAM172A homolog Source.73: DFBPPR12257 ---- Animal proteins ---- Polyunsaturated fatty acid lipoxygenase ALOX15 Source.74: DFBPPR12555 ---- Animal proteins ---- Glycogen debranching enzyme Source.75: DFBPPR13143 ---- Animal proteins ---- Toll-like receptor 9 Source.76: DFBPPR13268 ---- Animal proteins ---- Glycogen debranching enzyme Source.77: DFBPPR13576 ---- Animal proteins ---- Toll-like receptor 9 Source.78: DFBPPR13795 ---- Animal proteins ---- Keratin, type I microfibrillar 48 kDa, component 8C-1 Source.79: DFBPPR13823 ---- Animal proteins ---- Adrenocorticotropic hormone receptor Source.80: DFBPPR13858 ---- Animal proteins ---- Keratin, type I microfibrillar, 47.6 kDa Source.81: DFBPPR14406 ---- Marine protein ---- ATP synthase subunit a, chloroplastic Source.82: DFBPPR15169 ---- Microorganism protein ---- Protein VTS1 Source.83: DFBPPR7757 ---- Plant protein ---- Photosystem II protein D1 Source.84: DFBPPR7929 ---- Plant protein ---- Photosystem II protein D1 Source.85: DFBPPR8031 ---- Plant protein ---- Photosystem II protein D1 Source.86: DFBPPR8216 ---- Plant protein ---- Photosystem II protein D1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide CNW showed ABTS^⋅+ scavenging activity with the value of 4.18 μmol TE/μmol.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The protein powder (α-lactoalbumin, casein, rice bran protein, glutenin and hemp seed protein) was dispersed in deionized water (1:50, w/v) incubated at 37 ℃ and treated with pepsin, pepsin/protein ratio = 5% w/w at pH 2 for 120 min.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yin JY, Han YN, Liu MQ, Piao ZH, Zhang X, Xue YT, Zhang YH. Structure-guided discovery of antioxidant peptides bounded to the Keap1 receptor as hunter for potential dietary antioxidants. Food Chem. 2022 Mar 30;373(Pt A):130999. PMID: 34710694 DOI: 10.1016/j.foodchem.2021.130999
Other literature(s)	N.D
PubDate	2021