Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX1180(Antioxidative peptide)

DFBP ID	DFBPANOX1180
Peptide sequence	DHW
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asp-His-Trp

Single-letter amino acid

DHW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	456.46 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.96 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.5333^c

Hydrophilic residue ratio

33.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Food source
Organism/Source	Protein powder (α-lactoalbumin, casein, rice bran protein, glutenin and hemp seed protein)
Precursor protein	N.D
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1485 ---- Plant proteins ---- Pheophorbide a oxygenase, chloroplastic Source.2: DFBPPR1499 ---- Plant proteins ---- Protein kinase and PP2C-like domain-containing protein Source.3: DFBPPR1542 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-1 Source.4: DFBPPR1586 ---- Plant proteins ---- E3 ubiquitin-protein ligase GW2 Source.5: DFBPPR1671 ---- Plant proteins ---- MADS-box transcription factor 4 Source.6: DFBPPR1799 ---- Plant proteins ---- Aquaporin PIP1-1 Source.7: DFBPPR1811 ---- Plant proteins ---- Sulfhydryl oxidase 1 Source.8: DFBPPR1933 ---- Plant proteins ---- MADS-box transcription factor 2 Source.9: DFBPPR1955 ---- Plant proteins ---- Endoribonuclease Dicer homolog 3b Source.10: DFBPPR2156 ---- Plant proteins ---- UDP-glucose 6-dehydrogenase 2 Source.11: DFBPPR2293 ---- Plant proteins ---- Aquaporin PIP 1-3 Source.12: DFBPPR2477 ---- Plant proteins ---- Inorganic phosphate transporter 1-2 Source.13: DFBPPR2540 ---- Plant proteins ---- 23.2 kDa heat shock protein Source.14: DFBPPR2756 ---- Plant proteins ---- Probable aquaporin PIP1-2 Source.15: DFBPPR2794 ---- Plant proteins ---- FACT complex subunit SPT16 Source.16: DFBPPR2853 ---- Plant proteins ---- Barley B recombinant-like protein D Source.17: DFBPPR2856 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.18: DFBPPR2968 ---- Plant proteins ---- Probable aquaporin PIP2-7 Source.19: DFBPPR3140 ---- Plant proteins ---- ABC transporter G family member 51 Source.20: DFBPPR3154 ---- Plant proteins ---- Endoglucanase 7 Source.21: DFBPPR3356 ---- Plant proteins ---- Probable aquaporin PIP2-6 Source.22: DFBPPR3602 ---- Plant proteins ---- Coatomer subunit alpha-2 Source.23: DFBPPR3689 ---- Plant proteins ---- Probable inorganic phosphate transporter 1-7 Source.24: DFBPPR3736 ---- Plant proteins ---- Probable aquaporin PIP2-3 Source.25: DFBPPR3822 ---- Plant proteins ---- Coatomer subunit alpha-3 Source.26: DFBPPR3928 ---- Plant proteins ---- Coatomer subunit alpha-1 Source.27: DFBPPR4120 ---- Plant proteins ---- Probable aquaporin TIP4-3 Source.28: DFBPPR4145 ---- Plant proteins ---- Protein arginine N-methyltransferase 7 Source.29: DFBPPR4390 ---- Plant proteins ---- Spermidine hydroxycinnamoyltransferase 2 Source.30: DFBPPR4415 ---- Plant proteins ---- Putative ataxin-3 homolog Source.31: DFBPPR4435 ---- Plant proteins ---- Phospholipase A1-II 4 Source.32: DFBPPR4838 ---- Plant proteins ---- Protein DEHYDRATION-INDUCED 19 homolog 4 Source.33: DFBPPR4907 ---- Plant proteins ---- Protein GLUTELIN PRECURSOR ACCUMULATION 3 Source.34: DFBPPR5046 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 2 Source.35: DFBPPR5145 ---- Plant proteins ---- 22.0 kDa class IV heat shock protein Source.36: DFBPPR5418 ---- Plant proteins ---- Aquaporin PIP1-1 Source.37: DFBPPR5439 ---- Plant proteins ---- Aquaporin PIP1-2 Source.38: DFBPPR5468 ---- Plant proteins ---- Aquaporin PIP2-5 Source.39: DFBPPR5511 ---- Plant proteins ---- Aquaporin PIP2-1 Source.40: DFBPPR5655 ---- Plant proteins ---- Aquaporin PIP1-5 Source.41: DFBPPR5676 ---- Plant proteins ---- Aquaporin PIP1-3/PIP1-4 Source.42: DFBPPR5763 ---- Plant proteins ---- FACT complex subunit SPT16 Source.43: DFBPPR5797 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.44: DFBPPR5917 ---- Plant proteins ---- Aquaporin TIP4-4 Source.45: DFBPPR5969 ---- Plant proteins ---- Aquaporin PIP2-2 Source.46: DFBPPR6475 ---- Plant proteins ---- Probable aquaporin PIP-type 7a Source.47: DFBPPR6532 ---- Plant proteins ---- 22.7 kDa class IV heat shock protein Source.48: DFBPPR7182 ---- Plant proteins ---- Serine carboxypeptidase II-1 Source.49: DFBPPR7220 ---- Plant proteins ---- Chalcone synthase 1 Source.50: DFBPPR7675 ---- Milk proteins ---- Alpha-lactalbumin Source.51: DFBPPR7708 ---- Milk proteins ---- Late lactation protein B, LLP-B Source.52: DFBPPR15964 ---- Animal proteins ---- Aquaporin-1 Source.53: DFBPPR16133 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta Source.54: DFBPPR16207 ---- Animal proteins ---- Homeobox protein cut-like 1 Source.55: DFBPPR16211 ---- Animal proteins ---- Lysine-specific demethylase 5C Source.56: DFBPPR16232 ---- Animal proteins ---- Lysine-specific demethylase 5D Source.57: DFBPPR16242 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Source.58: DFBPPR16329 ---- Animal proteins ---- Neuropeptide Y receptor type 1 Source.59: DFBPPR16892 ---- Animal proteins ---- Acyl-coenzyme A synthetase ACSM1, mitochondrial Source.60: DFBPPR16901 ---- Animal proteins ---- Lens fiber membrane intrinsic protein Source.61: DFBPPR16979 ---- Animal proteins ---- Aquaporin-1 Source.62: DFBPPR16991 ---- Animal proteins ---- Osteocalcin Source.63: DFBPPR17016 ---- Animal proteins ---- cGMP-specific 3',5'-cyclic phosphodiesterase Source.64: DFBPPR17188 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Source.65: DFBPPR17307 ---- Animal proteins ---- Major histocompatibility complex class I-related gene protein Source.66: DFBPPR17493 ---- Animal proteins ---- Catechol O-methyltransferase Source.67: DFBPPR17516 ---- Animal proteins ---- Steroid 21-hydroxylase Source.68: DFBPPR17589 ---- Animal proteins ---- Aquaporin-2 Source.69: DFBPPR17602 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta Source.70: DFBPPR17718 ---- Animal proteins ---- Activin receptor type-2B Source.71: DFBPPR17785 ---- Animal proteins ---- MAP kinase-activated protein kinase 3 Source.72: DFBPPR17981 ---- Animal proteins ---- Retinol-binding protein 4 Source.73: DFBPPR17991 ---- Animal proteins ---- Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' Source.74: DFBPPR18056 ---- Animal proteins ---- Tyrosyl-DNA phosphodiesterase 2 Source.75: DFBPPR18218 ---- Animal proteins ---- Dual specificity protein phosphatase 6 Source.76: DFBPPR18447 ---- Animal proteins ---- DNA-(apurinic or apyrimidinic site) endonuclease 2 Source.77: DFBPPR18525 ---- Animal proteins ---- Lipoyltransferase 1, mitochondrial Source.78: DFBPPR18699 ---- Animal proteins ---- Lysyl oxidase homolog 4 Source.79: DFBPPR18813 ---- Animal proteins ---- Acyl-CoA synthetase short-chain family member 3, mitochondrial Source.80: DFBPPR18842 ---- Animal proteins ---- Protein arginine N-methyltransferase 7 Source.81: DFBPPR18866 ---- Animal proteins ---- Autophagy-related protein 9A Source.82: DFBPPR18967 ---- Animal proteins ---- Krev interaction trapped protein 1 Source.83: DFBPPR19036 ---- Animal proteins ---- Elongation factor 2 Source.84: DFBPPR19122 ---- Animal proteins ---- Carbonic anhydrase 3 Source.85: DFBPPR19350 ---- Animal proteins ---- Neuropeptide Y receptor type 1 Source.86: DFBPPR19390 ---- Animal proteins ---- E3 ubiquitin-protein ligase TM129 Source.87: DFBPPR19408 ---- Animal proteins ---- Tumor protein p63-regulated gene 1-like protein Source.88: DFBPPR19442 ---- Animal proteins ---- Eukaryotic translation initiation factor 3 subunit G Source.89: DFBPPR19449 ---- Animal proteins ---- SCO-spondin Source.90: DFBPPR19682 ---- Animal proteins ---- F-box only protein 2 Source.91: DFBPPR19710 ---- Animal proteins ---- Beta-galactosidase Source.92: DFBPPR19742 ---- Animal proteins ---- Phosphoglucomutase-1 Source.93: DFBPPR19794 ---- Animal proteins ---- Bone morphogenetic protein 15 Source.94: DFBPPR19938 ---- Animal proteins ---- Serine/threonine-protein phosphatase CPPED1 Source.95: DFBPPR20022 ---- Animal proteins ---- Voltage-dependent calcium channel gamma-1 subunit Source.96: DFBPPR20109 ---- Animal proteins ---- Ovarian cancer G-protein coupled receptor 1 Source.97: DFBPPR20359 ---- Animal proteins ---- Glutathione S-transferase theta-1 Source.98: DFBPPR20383 ---- Animal proteins ---- Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial Source.99: DFBPPR20692 ---- Animal proteins ---- ELMO domain-containing protein 3 Source.100: DFBPPR20865 ---- Animal proteins ---- Methionine adenosyltransferase 2 subunit beta Source.101: DFBPPR21562 ---- Animal proteins ---- COP9 signalosome complex subunit 6 Source.102: DFBPPR21799 ---- Animal proteins ---- Major vault protein Source.103: DFBPPR22231 ---- Animal proteins ---- DnaJ homolog subfamily C member 12 Source.104: DFBPPR22423 ---- Animal proteins ---- Transmembrane protein 45B Source.105: DFBPPR22512 ---- Animal proteins ---- IQ domain-containing protein C Source.106: DFBPPR8728 ---- Animal proteins ---- Aquaporin-1 Source.107: DFBPPR9051 ---- Animal proteins ---- Retinol-binding protein 4 Source.108: DFBPPR9105 ---- Animal proteins ---- Lysine-specific demethylase 5C Source.109: DFBPPR9289 ---- Animal proteins ---- Carbonic anhydrase 3 Source.110: DFBPPR9309 ---- Animal proteins ---- Sperm flagellar protein 2 Source.111: DFBPPR9554 ---- Animal proteins ---- Voltage-dependent calcium channel gamma-1 subunit Source.112: DFBPPR9600 ---- Animal proteins ---- P2Y purinoceptor 2 Source.113: DFBPPR9609 ---- Animal proteins ---- Neuropeptide Y receptor type 1 Source.114: DFBPPR9821 ---- Animal proteins ---- COP9 signalosome complex subunit 6 Source.115: DFBPPR9983 ---- Animal proteins ---- Fibrinogen alpha chain Source.116: DFBPPR10029 ---- Animal proteins ---- Activin receptor type-2B Source.117: DFBPPR10092 ---- Animal proteins ---- Retinol-binding protein 4 Source.118: DFBPPR10231 ---- Animal proteins ---- Basigin Source.119: DFBPPR10262 ---- Animal proteins ---- Dorsalin-1 Source.120: DFBPPR10266 ---- Animal proteins ---- Transcription factor GATA-6 Source.121: DFBPPR10397 ---- Animal proteins ---- Tyrosine-protein kinase receptor TYRO3 Source.122: DFBPPR10410 ---- Animal proteins ---- Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' Source.123: DFBPPR10423 ---- Animal proteins ---- Sortilin-related receptor Source.124: DFBPPR10435 ---- Animal proteins ---- Spectrin alpha chain, non-erythrocytic 1 Source.125: DFBPPR10579 ---- Animal proteins ---- Lysine-specific demethylase 5B Source.126: DFBPPR10750 ---- Animal proteins ---- Phosphatidylserine synthase 2 Source.127: DFBPPR10765 ---- Animal proteins ---- Tyrosyl-DNA phosphodiesterase 2 Source.128: DFBPPR10901 ---- Animal proteins ---- Protein arginine N-methyltransferase 7 Source.129: DFBPPR10934 ---- Animal proteins ---- Glutathione S-transferase theta-1 Source.130: DFBPPR10980 ---- Animal proteins ---- Elongation factor 2 Source.131: DFBPPR11109 ---- Animal proteins ---- Fibrinogen-like protein 1-like protein Source.132: DFBPPR11680 ---- Animal proteins ---- GTPase Era, mitochondrial Source.133: DFBPPR12252 ---- Animal proteins ---- Phosphoglucomutase-1 Source.134: DFBPPR12315 ---- Animal proteins ---- Voltage-dependent calcium channel gamma-1 subunit Source.135: DFBPPR12380 ---- Animal proteins ---- N-acylethanolamine-hydrolyzing acid amidase Source.136: DFBPPR12522 ---- Animal proteins ---- Alpha-lactalbumin Source.137: DFBPPR12828 ---- Animal proteins ---- Retinol-binding protein 4 Source.138: DFBPPR12848 ---- Animal proteins ---- Sarcoplasmic reticulum histidine-rich calcium-binding protein Source.139: DFBPPR13175 ---- Animal proteins ---- Catechol O-methyltransferase Source.140: DFBPPR13227 ---- Animal proteins ---- Carbonic anhydrase 3 Source.141: DFBPPR13232 ---- Animal proteins ---- Osteocalcin Source.142: DFBPPR13374 ---- Animal proteins ---- Retinol-binding protein 4 Source.143: DFBPPR13587 ---- Animal proteins ---- Aquaporin-1 Source.144: DFBPPR13662 ---- Animal proteins ---- Aquaporin-2 Source.145: DFBPPR13785 ---- Animal proteins ---- Bone morphogenetic protein 15 Source.146: DFBPPR14136 ---- Marine protein ---- Lysine-specific demethylase 8 Source.147: DFBPPR14350 ---- Marine protein ---- 3-oxoacyl-[acyl-carrier-protein] synthase 3 Source.148: DFBPPR14550 ---- Marine protein ---- Complement C3 Source.149: DFBPPR14647 ---- Marine protein ---- Retinol-binding protein 4-A Source.150: DFBPPR14648 ---- Marine protein ---- Retinol-binding protein 4-B Source.151: DFBPPR15041 ---- Microorganism protein ---- Autophagy protein 5 Source.152: DFBPPR15065 ---- Microorganism protein ---- Lactose regulatory protein LAC9 Source.153: DFBPPR15074 ---- Microorganism protein ---- tRNA-dihydrouridine(47) synthase [NAD(P)(+)] Source.154: DFBPPR15182 ---- Microorganism protein ---- Mitochondrial transcription factor 1 Source.155: DFBPPR15456 ---- Microorganism protein ---- RNA polymerase II holoenzyme cyclin-like subunit Source.156: DFBPPR15472 ---- Microorganism protein ---- Enhancer of polycomb-like protein 1 Source.157: DFBPPR15483 ---- Microorganism protein ---- Elongation factor 2 Source.158: DFBPPR15681 ---- Microorganism protein ---- Protein SWT21 Source.159: DFBPPR7752 ---- Plant protein ---- Trans-cinnamate 4-monooxygenase Source.160: DFBPPR7820 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta Source.161: DFBPPR7946 ---- Plant protein ---- Aquaporin PIP1.1 Source.162: DFBPPR8039 ---- Plant protein ---- Linoleate 9S-lipoxygenase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	The peptide DHW showed ABTS^⋅+ scavenging activity with the value of 4.23 μmol TE/μmol.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The protein powder (α-lactoalbumin, casein, rice bran protein, glutenin and hemp seed protein) was dispersed in deionized water (1:50, w/v) incubated at 37 ℃ and treated with pepsin, pepsin/protein ratio = 5% w/w at pH 2 for 120 min.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yin JY, Han YN, Liu MQ, Piao ZH, Zhang X, Xue YT, Zhang YH. Structure-guided discovery of antioxidant peptides bounded to the Keap1 receptor as hunter for potential dietary antioxidants. Food Chem. 2022 Mar 30;373(Pt A):130999. PMID: 34710694 DOI: 10.1016/j.foodchem.2021.130999
Other literature(s)	N.D
PubDate	2021