Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANOX1205(Antioxidative peptide)

DFBP ID	DFBPANOX1205
Peptide sequence	RGLSK
Type	Native peptide
Peptide/Function name	Antioxidative peptide

Function-activity relationship

Main bioactivity	Antioxidative activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Arg-Gly-Leu-Ser-Lys

Single-letter amino acid

RGLSK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	559.66 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.54 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.1600^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Food
Organism/Source	Pixian broad bean paste (PBBP)
Precursor protein	The water-soluble peptide extracts of PBBP
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2688 ---- Plant proteins ---- Regulatory-associated protein of TOR 2 Source.2: DFBPPR2765 ---- Plant proteins ---- Regulatory-associated protein of TOR 1 Source.3: DFBPPR18311 ---- Animal proteins ---- Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha Source.4: DFBPPR18806 ---- Animal proteins ---- Pseudouridylate synthase 7 homolog Source.5: DFBPPR19499 ---- Animal proteins ---- Transcription factor MafG Source.6: DFBPPR21437 ---- Animal proteins ---- Distal membrane-arm assembly complex protein 2 Source.7: DFBPPR22473 ---- Animal proteins ---- BTB/POZ domain-containing protein KCTD19 Source.8: DFBPPR8889 ---- Animal proteins ---- Hexokinase-2 Source.9: DFBPPR11133 ---- Animal proteins ---- Transcription factor MafG Source.10: DFBPPR11208 ---- Animal proteins ---- Transcription factor MafF Source.11: DFBPPR11745 ---- Animal proteins ---- Digestive organ expansion factor homolog Source.12: DFBPPR12430 ---- Animal proteins ---- Junctophilin-2 Source.13: DFBPPR13172 ---- Animal proteins ---- Hexokinase-2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antioxidative activity	Peptide RGLSK presented antioxidant capacity (ABTS^•+ assay, 12 μmol TE/g, DPPH• assay, 26.19 %).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Pixian broad bean paste employed in this study was provided by Sichuan Pixian Douban Co., Ltd. (Pixian, Sichuan, China) and held at −20 ℃.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI2252
[D2]	DFBPMUFU0650

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Li, M., Fan, W. and Xu, Y. (2021) Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste. LWT, 151, 112221. DOI: 10.1016/j.lwt.2021.112221
Other literature(s)	N.D
PubDate	2021