Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANPE0003(Antiviral peptide)

DFBP ID	DFBPANPE0003
Peptide sequence	LEYS
Type	Native peptide
Peptide/Function name	Antiviral peptide

Function-activity relationship

Main bioactivity	Antiviral activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Glu-Tyr-Ser

Single-letter amino acid

LEYS

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	510.53 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.4500^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Oyster
Precursor protein	Oyster proteins
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1122 ---- Plant proteins ---- Tryptamine 5-hydroxylase Source.2: DFBPPR1157 ---- Plant proteins ---- MADS-box transcription factor 17 Source.3: DFBPPR1183 ---- Plant proteins ---- MADS-box transcription factor 18 Source.4: DFBPPR1622 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.5: DFBPPR2351 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Source.6: DFBPPR3000 ---- Plant proteins ---- Kinesin-like protein KIN-14L Source.7: DFBPPR3461 ---- Plant proteins ---- 50S ribosomal protein L18, chloroplastic Source.8: DFBPPR3907 ---- Plant proteins ---- Cyclin-A1-4 Source.9: DFBPPR4902 ---- Plant proteins ---- Endoribonuclease Dicer homolog 1 Source.10: DFBPPR5064 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.11: DFBPPR5696 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.12: DFBPPR5708 ---- Plant proteins ---- 3-hydroxyindolin-2-one monooxygenase Source.13: DFBPPR5762 ---- Plant proteins ---- CRS2-associated factor 1, chloroplastic Source.14: DFBPPR5766 ---- Plant proteins ---- MADS-box protein ZMM17 Source.15: DFBPPR6398 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.16: DFBPPR6653 ---- Plant proteins ---- Sedoheptulose-1,7-bisphosphatase, chloroplastic Source.17: DFBPPR6809 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.18: DFBPPR7470 ---- Plant proteins ---- ATP synthase subunit alpha, mitochondrial Source.19: DFBPPR16027 ---- Animal proteins ---- Rho GTPase-activating protein 35 Source.20: DFBPPR16236 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1D Source.21: DFBPPR16274 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 37 Source.22: DFBPPR17330 ---- Animal proteins ---- Unconventional myosin-X Source.23: DFBPPR17493 ---- Animal proteins ---- Catechol O-methyltransferase Source.24: DFBPPR17957 ---- Animal proteins ---- E3 ubiquitin-protein ligase HACE1 Source.25: DFBPPR17991 ---- Animal proteins ---- Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' Source.26: DFBPPR18288 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 37 Source.27: DFBPPR19575 ---- Animal proteins ---- Acylphosphatase-2 Source.28: DFBPPR20172 ---- Animal proteins ---- NF-kappa-B inhibitor zeta Source.29: DFBPPR20200 ---- Animal proteins ---- N-lysine methyltransferase SETD6 Source.30: DFBPPR20410 ---- Animal proteins ---- Protein AF1q Source.31: DFBPPR20840 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 3 Source.32: DFBPPR21316 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit alpha Source.33: DFBPPR21334 ---- Animal proteins ---- LisH domain-containing protein ARMC9 Source.34: DFBPPR22165 ---- Animal proteins ---- Coiled-coil domain-containing protein 106 Source.35: DFBPPR22228 ---- Animal proteins ---- Rho GTPase-activating protein 36 Source.36: DFBPPR22282 ---- Animal proteins ---- Optic atrophy 3 protein homolog Source.37: DFBPPR8651 ---- Animal proteins ---- cAMP-dependent protein kinase catalytic subunit alpha Source.38: DFBPPR9187 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 37 Source.39: DFBPPR9228 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1D Source.40: DFBPPR9512 ---- Animal proteins ---- Acylphosphatase-2 Source.41: DFBPPR9820 ---- Animal proteins ---- WD repeat-containing protein 62 Source.42: DFBPPR10206 ---- Animal proteins ---- Unconventional myosin-VI Source.43: DFBPPR10363 ---- Animal proteins ---- E3 ubiquitin-protein ligase HACE1 Source.44: DFBPPR10410 ---- Animal proteins ---- Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' Source.45: DFBPPR10459 ---- Animal proteins ---- Reelin Source.46: DFBPPR10466 ---- Animal proteins ---- Steroid 17-alpha-hydroxylase/17,20 lyase Source.47: DFBPPR10493 ---- Animal proteins ---- Ubiquitin-like modifier-activating enzyme ATG7 Source.48: DFBPPR10539 ---- Animal proteins ---- Netrin receptor UNC5C Source.49: DFBPPR10750 ---- Animal proteins ---- Phosphatidylserine synthase 2 Source.50: DFBPPR11251 ---- Animal proteins ---- Transcriptional enhancer factor TEF-5 Source.51: DFBPPR11288 ---- Animal proteins ---- AKT-interacting protein Source.52: DFBPPR11663 ---- Animal proteins ---- Limbic system-associated membrane protein Source.53: DFBPPR11832 ---- Animal proteins ---- N-lysine methyltransferase SETD6 Source.54: DFBPPR12552 ---- Animal proteins ---- Acylphosphatase-2 Source.55: DFBPPR12570 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1D Source.56: DFBPPR12581 ---- Animal proteins ---- Potassium voltage-gated channel subfamily B member 2 Source.57: DFBPPR12752 ---- Animal proteins ---- Complement component C8 beta chain Source.58: DFBPPR13175 ---- Animal proteins ---- Catechol O-methyltransferase Source.59: DFBPPR13324 ---- Animal proteins ---- Acylphosphatase-2 Source.60: DFBPPR13774 ---- Animal proteins ---- Trophoblast Kunitz domain protein 1 Source.61: DFBPPR14149 ---- Marine protein ---- AKT-interacting protein Source.62: DFBPPR14343 ---- Marine protein ---- Anthranilate synthase component 2 Source.63: DFBPPR15039 ---- Microorganism protein ---- ATP-dependent RNA helicase SUB2 Source.64: DFBPPR15160 ---- Microorganism protein ---- Palmitoyltransferase PFA3 Source.65: DFBPPR15258 ---- Microorganism protein ---- Invertase Source.66: DFBPPR15334 ---- Microorganism protein ---- Probable kinetochore protein NUF2 Source.67: DFBPPR15398 ---- Microorganism protein ---- Transcription activator of gluconeogenesis ERT1 Source.68: DFBPPR15468 ---- Microorganism protein ---- Mitochondrial inner membrane magnesium transporter LPE10 Source.69: DFBPPR15763 ---- Microorganism protein ---- ATPase expression protein 3 Source.70: DFBPPR8093 ---- Plant protein ---- ATP synthase subunit alpha, mitochondrial
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antiviral activity	Chemically synthesized Leu-Glu-Tyr-Ser showed HIV-1 protease (EC 3.4.23.16) inhibitory activity with an IC₅₀ value of 4800 nM.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CO)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptides inhibiting HIV-1 protease were isolated from the hydrolysate of oyster (Crassostrea gigas) proteins prepared with thermolysin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Lee TG, Maruyama S. Isolation of HIV-1 protease-inhibiting peptides from thermolysin hydrolysate of oyster proteins. Biochem Biophys Res Commun. 1998 Dec 30;253(3):604-8. PMID: 9918775 DOI: 10.1006/bbrc.1998.9824
Other literature(s)	N.D
PubDate	1998